A5076690824- Immune Cell Function and Interaction
- Hepatitis Viruses Studies and Epidemiology
- Liver Diseases and Immunity
- Monoclonal and Polyclonal Antibodies Research
- Macrophage Migration Inhibitory Factor
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Acute Lymphoblastic Leukemia research
- Epigenetics and DNA Methylation
- CAR-T cell therapy research
- Immune cells in cancer
- COVID-19 epidemiological studies
- Diabetes and associated disorders
- Nanoparticle-Based Drug Delivery
- Folate and B Vitamins Research
- Mast cells and histamine
- Food Allergy and Anaphylaxis Research
- Cancer, Hypoxia, and Metabolism
- Hepatitis B Virus Studies
- Biochemical and Molecular Research
- Eosinophilic Disorders and Syndromes
- Biosensors and Analytical Detection
- Pancreatic function and diabetes
- Protist diversity and phylogeny
- Infective Endocarditis Diagnosis and Management
University of Pittsburgh
2017-2025
B.J. Medical College
2019-2021
PharmacoGenetics (China)
2019
University of Agricultural Sciences, Dharwad
2017
ICMR-National Institute of Virology
2011-2016
Aurigene Discovery Technologies (India)
2016
Duke University
2012
EPIC1-EZH2 axis activation emerges as a targetable mechanism for tumor immune evasion and immunotherapy resistance.
Objectives To study the percentage seropositivity for SARS-CoV-2 to understand pandemic status and predict future situations in Ahmedabad. Study design Cross-sectional study. Settings Field area of Ahmedabad Municipal Corporation. Participants More than 30 000 individuals irrespective their age, sex, acute/past COVID-19 infection participated serosurvey which covered all 75 Urban Primary Health Centres (UPHCs) across 48 wards 7 zones city. also involved healthcare workers (HCWs) from...
Type 1 diabetes (T1D) is an autoimmune disease characterized by the destruction of insulin-producing pancreatic β-cells their own immune system, resulting in lifelong insulin deficiency. Continuous exogenous replacement therapy current standard care for T1D. Transplantation primary islets or entire pancreas a viable remedy managing patients with However, this strategy not feasible due to several obstacles, including scarcity donors, islet cells, and poor vascular engraftment...
TNFα inhibitor (TNFi) immunogenicity in rheumatoid arthritis (RA) is a major obstacle to its therapeutic effectiveness. Although methotrexate (MTX) can mitigate TNFi immunogenicity, adverse effects necessitate alternative strategies. Targeting nuclear factor of activated T cells (NFAT) transcription factors may protect against biologic immunogenicity. Therefore, developing potent NFAT suppress this offer an MTX. We performed structure-based virtual screen the NFATC2 crystal structure...
Aim:The present study was done to determine the activity of licorice root extract on Streptococcus mutans (S. mutans) in comparison chlorhexidine and fluoride mouthwash. Materials methods:In current study, different concentrations aqueous ethanolic were subjected microbiological assay zone inhibition determined against S. by agar ditch method.Minimum inhibitory concentration (MIC) solution obtained using broth dilution method method.Chlorhexidine mouthwash kept as a positive control...
Background Hepatitis E is a major public health problem in the developing countries. Pathogenesis of hepatitis virus (HEV) infection poorly understood. Methods This case-control study included 124 patients (46 acute and 78 recovered), 9 with prior exposure to HEV 71 anti-HEV negative healthy controls. induced CTL response by Elispot, cytokines/chemokines quantitation Milliplex assay peripheral CD4+ & CD8+ T cell frequencies flow cytometry were assessed. Results Among patient categories,...
Asparaginase is an important drug for the treatment of leukemias. However, anti-asparaginase antibodies often develop, which can decrease asparaginase levels and increase risk relapse. The aim this study to identify immunoglobulin isotypes receptors responsible hypersensitivities. Mice immunized with developed IgG1 IgE antibodies, challenging sensitized mice induced severe hypersensitivity reactions. Flow cytometry analysis indicated that macrophages/monocytes, neutrophils, basophils bind ex...
Excessive complement activation has been implicated in the pathogenesis of coronavirus disease 2019 (COVID-19), but mechanisms leading to this response remain unclear.We measured plasma levels key markers, severe acute respiratory syndrome 2 (SARS-CoV-2) RNA and antibodies against SARS-CoV-2 seasonal human common cold coronaviruses (CCCs) hospitalized patients with COVID-19 moderate (n = 18) critical severity 37) healthy controls 10).We confirmed that is systemically increased associated a...
Summary Hepatitis E virus ( HEV ) is a major cause of self‐limiting acute viral hepatitis in several developing countries. Elevated levels peripheral CD 4 + 25 F oxp3 , − Foxp3 and rise IL ‐10 have been associated with the involvement regulatory T cells reg). The functional role same yet elusive. In current study, we assessed (i) expression by real‐time PCR flow cytometry, (ii) antigen‐specific TGF ‐β ELISA (iii) analysis reg (iv) reg‐associated conventional phenotypes cytometry 54 patients,...
Abstract The family of nuclear factor activated T cells (NFAT) transcription factors plays a critical role in mediating immune responses. Our previous clinical pharmacogenetic studies suggested that NFATC2 is associated with the risk hypersensitivity reactions to chemotherapeutic agent L-asparaginase (ASNase) worsen outcomes during treatment pediatric acute lymphoblastic leukemia. We therefore hypothesized genetic inhibition would protect against development anti-ASNase antibodies and ASNase...
To improve the current knowledge on involvement of peripheral lymphocytes in hepatitis E virus (HEV) associated pathogenesis, we analyzed alterations (1) immunophenotypic expressions (by flow cytometry) and (2) gene expression patterns TaqMan Low Density Array) activatory, inhibitory, integrin, homing, ectonucleotidase machinery, costimulatory, inflammatory markers, T regulatory cells (Treg) cytokines HEV rORF2p stimulated unstimulated PBMCs 43 acute patients, 30 recovered individuals,...
PEGylated-l-asparaginase (PEG-ASNase) is a chemotherapeutic agent used to treat pediatric acute lymphoblastic leukemia (ALL). Its use avoided in adults due its high risk of liver injury including hepatic steatosis, with obesity and older age considered factors the injury. Our study aims elucidate mechanism PEG-ASNase-induced Mice received 1500 U/kg PEG-ASNase were sacrificed 1, 3, 5, 7 days after drug administration. Liver triglycerides quantified, plasma bilirubin, ALT, AST, non-esterified...
Elevated levels of transforming growth factor-β1 (TGF-β1) and its positive correlation with Foxp3 expression in hepatitis E patients have indicated involvement TGF-β1 pathogenesis. The current study determined polymorphisms gene, plasma T effector (Teff) cell proliferation explored their association a case control study. Polymorphisms three selected sites (- 509C > T, + 869T C 915G C) gene by PCR & restriction fragment length polymorphism methods, quantitation ELISA Teff (CD4 CD25 -) CFSE...
Abstract: Food allergy is one of the world's most serious health problems, which needs new and safer approaches to resolve it. The rapid progress nanotechnology tackle problem food allergen has generated a hope. Nanoparticles can be used as unique system detect allergens. Furthermore, offers allergen-delivery systems based on nanoparticles potential adjuvants for allergen-specific immunotherapy. In addition, use novel using provides harmless potentially more effective way treatment allergic...
Asparaginase (ASNase) is an important drug for the treatment of leukemias. However, hypersensitivity to ASNase can increase risk leukemia relapse. Two mechanisms have been identified in mice. The existence a pathway involving anti-ASNase IgG and Fc-γ receptor III (Fc-γRIII) implies that immune complexes (ICs) could directly induce hypersensitivity. aim this study was detect ICs mice after reactions determine their role Protein G beads were used plasma by flow cytometry. Anti-ASNase purified...
Patients with rheumatoid arthritis (RA) are frequently treated anti–tumor necrosis factor-<i>α</i> immunoglobulin therapy but develop neutralizing antibodies against these drugs, necessitating therapeutic monitoring of drug concentrations and anti-drug antibodies. RA have multiple factors related to their autoimmune disposition that interfere conventionally used methods detect Currently deployed analytical significant limitations hinder clinical interpretation and/or routine use, no method...