A5043302366- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrochemical Analysis and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Mass Spectrometry Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Protein Structure and Dynamics
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Analytical chemistry methods development
- Nanopore and Nanochannel Transport Studies
- Photosynthetic Processes and Mechanisms
- Electrochemical sensors and biosensors
- ZnO doping and properties
- Antibiotics Pharmacokinetics and Efficacy
- Enzyme Structure and Function
- Protein purification and stability
- Nonlinear Optical Materials Research
- CAR-T cell therapy research
- Viral Infections and Immunology Research
- Chalcogenide Semiconductor Thin Films
- Analytical Chemistry and Chromatography
- Virus-based gene therapy research
IGM Biosciences (United States)
2022-2023
Merck & Co., Inc., Rahway, NJ, USA (United States)
2021-2022
University of Michigan
2014-2020
Ann Arbor Center for Independent Living
2017-2020
United States Military Academy
2020
Mustansiriyah University
2018
Al-Azhar University
2010-2014
Seoul National University
2011-2013
Prince Sattam Bin Abdulaziz University
2012-2013
University of Tasmania
2008-2011
Abstract Capillary electrophoresis has been alive for over two decades now; yet, its sensitivity is still regarded as being inferior to that of more traditional methods separation such HPLC. As such, it unsurprising overcoming this issue generates much scientific interest. This review continues update series reviews, first published in Electrophoresis 2007, with an 2009 and covers material through June 2010. It includes developments the fields stacking, covering all from field‐amplified...
Abstract Poor sensitivity is still considered to be one of the major limitations electrophoresis, which surprising given power, flexibility and versatility many approaches on‐line concentration that have developed over last 20 years. This a very active area interest this review will cover developments in field two years since ( Electrophoresis 2007, 28, 254–281) through June 2008. It includes fields stacking, covering all methods from field‐amplified sample stacking large volume ITP, dynamic...
Western blotting is a commonly used protein assay that combines the selectivity of electrophoretic separation and immunoassay. The technique limited by long time, manual operation with mediocre reproducibility, large sample consumption, typically 10-20 μg per assay. blots are also usually to measure only one an additional housekeeping for normalization. Measurement multiple proteins possible; however, it requires stripping membranes antibody then reprobing second antibody. Miniaturized...
Capillary electrophoresis (CE) has been identified as a useful platform for detecting, quantifying, and screening modulators of protein–protein interactions (PPIs). In this method, one protein binding partner is labeled with fluorophore, the partners are mixed, then, complex separated from free to allow direct determination bound ratios. Although it possesses many advantages PPI studies, method limited by need have separation conditions that both prevent adsorption capillary maintain during...
Abstract Electrokinetic supercharging, a powerful on‐line preconcentration technique in CE, was for the first time hyphenated with ESI‐MS concentration and separation of five hypolipidaemic drugs. The electrophoretic performed co‐EOF mode using EOF reversal agent, hexadimethrine bromide, ammonium bicarbonate electrolyte, pH 9.00. ionic strength amount methanol buffer were optimised multivariate manner artificial neural networks, optimal conditions being 60 mM containing 60% methanol,...
Here protein cross-linking capillary electrophoresis is demonstrated for a wide range of protein–protein interactions including weak and multimeric oligomers.
Electrokinetic supercharging is one of the most powerful sample-stacking methods that combines field amplified sample injection and transient ITP. In counter-flow electrokinetic supercharging, a constant counter pressure applied during in order to counterbalance movement injected zone. As result, there will be pronounced increase amount portion capillary available for electrophoresis. this report, optimization factors such as electric application voltage current modes, magnitude pressure,...
Electrokinetic supercharging (EKS) is known as one of the most effective online electrophoretic preconcentration techniques, though pairing with it mass spectrometry has presented challenges. Here, EKS successfully paired ESI-MS/MS to provide a sensitive and robust method for analysis biogenic amines in biological samples. Injection parameters including electric field strength buffer compositions used separation focusing were investigated achieve suitable resolution, high sensitivity,...
Western blotting is a widely used protein assay platform, but the technique requires long analysis times and multiple manual steps. Microfluidic systems are currently being explored for increased automation reduction of times, sample volumes, reagent consumption western blots. Previous work has demonstrated that proteins separated by microchip electrophoresis can be captured on membranes dragging outlet across membrane. This process reduces separation transfer time blot to few minutes. To...
A prototype strain of Coxsackievirus A21 (CVA21) is being evaluated as an oncolytic virus immunotherapy. CVA21 preferentially lyses cells that upregulate the expression intercellular adhesion molecule 1, which includes some types tumor cells. has icosahedral capsid structure made up 60 protein subunits encapsidating a viral RNA genome with particle diameter size 30 nm. Rapid and robust analytical methods to quantify total, empty, full particles are important support process development, meet...
The capacity of SARS-CoV-2 to evolve poses challenges conventional prevention and treatment options such as vaccination monoclonal antibodies, they rely on viral receptor binding domain (RBD) sequences from previous strains. Additionally, animal CoVs, especially those the SARS family, are now appreciated a constant pandemic threat. We present here new antiviral approach featuring inhalation delivery recombinant trap composed ten copies angiotensin-converting enzyme 2 (ACE2) fused IgM Fc....
Abstract The capacity of SARS-CoV-2 to evolve poses challenges conventional prevention and treatment options such as vaccination monoclonal antibodies, they rely on viral receptor binding domain (RBD) sequences from previous strains. Additionally, animal CoVs, especially those the SARS family, are now appreciated a constant pandemic threat. We present here new antiviral approach featuring inhalation delivery recombinant trap composed ten copies angiotensin-converting enzyme 2 (ACE2) fused...