A5023950859- Probiotics and Fermented Foods
- Gut microbiota and health
- Microbial Metabolites in Food Biotechnology
- Digestive system and related health
- Infant Nutrition and Health
- Food composition and properties
- Escherichia coli research studies
- Aquaculture disease management and microbiota
- Antimicrobial Peptides and Activities
- Animal Nutrition and Physiology
- Intestinal Malrotation and Obstruction Disorders
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Gastrointestinal motility and disorders
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Biochemical Analysis and Sensing Techniques
- Diet and metabolism studies
- Biochemical and Structural Characterization
- Vibrio bacteria research studies
- Clinical Nutrition and Gastroenterology
- Mycobacterium research and diagnosis
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Advanced Drug Delivery Systems
UNSW Sydney
2016-2025
Singapore Centre for Environmental Life Sciences Engineering
2022-2025
Nanyang Technological University
2018-2025
The University of Western Australia
2023
Baptist Memorial Hospital for Women
2023
King Edward Memorial Hospital
2023
Environmental Earth Sciences
2021-2023
Essentia Health
2022
University of Minnesota, Duluth
2022
University of Minnesota
2022
To optimize a spray coating process for the production of encapsulated microspheres containing viable Bifidobacterium cells and to determine whether readily gelatinized modified starch used in this study improved bacterial survival foods or under acid conditions.An air inlet temperature 100 degrees C was demonstrated be optimal drying process, as it afforded good drying, low outlet temperatures (45 C) resulted less than 1 log reduction bifidobacteria numbers during drying. Maximum recovery...
More than 400 isolates from the intestine and external surface of farmed Scophtalmus maximus as well fish food hatchery water were screened for inhibitory effects against pathogen Vibrio anguillarum HI 11345 seven other pathogens. The bacteria with then characterized regard to their sites colonization, especially intestinal regions within each region. Of total number bacterial intestine, 28% V. 11345. A marine biochemical assay was used order strains into different phena. Most found in rinse...
Of more than 400 bacteria isolated from turbot (Scophthalmus maximus), 89 have previously been shown to inhibit the in vitro growth of fish pathogen Vibrio anguillarum. The aim present study was investigate potential seven these strains, as well intestinal isolates (four strains) a closely related fish, dab (Limanda limanda), for colonizing farmed means protecting host infection by V. In addition, inhibitory effect strains on further studied. Colonization measured capacity adhere and grow...
ABSTRACT An adhesion-promoting protein involved in the binding of Lactobacillus fermentum strain 104R to small intestinal mucus from piglets and partially purified gastric mucin was isolated characterized. Spent culture supernatant fluid bacterial cell wall extracts were fractionated by ammonium sulfate precipitation gel filtration. The active fraction affinity chromatography. detected fractions adhesion inhibition dot blot assays visualized polyacrylamide electrophoresis (PAGE), sodium...
A non‐virulent Carnobacterium sp., designated strain K1, isolated from the gastrointestinal tract of Atlantic salmon, Salmo salar L., which produced inhibitory substances against bacterial fish pathogens, was examined in vitro for characteristics important colonization and vivo persistence after oral dosing. In growth experiments showed that cells this were metabolically active both intestinal mucus faeces salmonids. The production inhibitors two common pathogens Vibrio anguillarum Aeromonas...
Background Probiotic supplementation significantly reduces the risk of necrotising enterocolitis (NEC) and all cause mortality in preterm neonates. Independent quality assessment is important before introducing routine probiotic this cohort. Aim To assess product quality, confirm that Bifidobacterium breve (B. breve) M-16V will increase fecal B. counts without adverse effects. Methods Participants Strain identity (16S rRNA gene sequencing), viability over 2 year shelf-life were confirmed,...
Abstract Extracellular polymeric substances (EPS) are core biofilm components, yet how they mediate interactions within and contribute to the structuring of biofilms is largely unknown, particularly for non-culturable microbial communities that predominate in environmental habitats. To address this knowledge gap, we explored role EPS an anaerobic ammonium oxidation (anammox) biofilm. An extracellular glycoprotein, BROSI_A1236, from anammox bacterium, formed envelopes around cells, supporting...
Enteropathogenic Escherichia coli K88 colonizing the piglet ileum adhere to mucosa by fimbrial appendages. A recent study in our laboratory has implicated indigenous lactobacilli suppression of colonization potential enteropathogenic E. as measured adhesion ileal mucus. The aim this was investigate effect Lactobacillus spp. porcine origin on fimbriae coli. With an vitro assay, K88ab strain G1108E and K88ac 1107 35-day-old mucus studied presence spent culture fluid Detailed studies focused...
Abstract The role of the intestinal tract in Vibrio anguillarum infection turbot, Scophthalmus maximus (L.), fingerlings was investigated two vivo models and possible mechanisms involved were studied vitro. Viable V. cells detected spleens from more than 50% fish administered pathogen orally or rectally, suggesting that is a portal entry for V anguillarum. In transmission electron micrographs, ‐ like seen close to rectal epithelium, penetration mucus layer, but no epithelial cell endocytosis...
The mechanism of adhesion Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium was investigated. Adhesion inhibition tests involving chelators, monosaccharides, periodate and concanavalin A the use bacteria grown in presence tunicamycin failed clarify adhesive mechanism. Washed bacterial cells had reduced capacity, except spent broth culture supernatant fraction or cell washings. Spent fractions erythrosine-supplemented did not enhance washed cells. adhesion-promoting...
The adhesion of Lactobacillus fermentum 104-R and the variant strain 104-S to porcine gastric squamous epithelium was investigated. An epithelium-specific detected for 104-S; however, expressed enhanced capacity control surfaces polystyrene bovine serum albumin. To characterize adhesive determinants, bacterial cells were exposed various treatments. pattern in buffers pH values ranging from 2 7 determined. greater a buffer with higher value. On other hand, rather unaffected by change pH. or...
The possibility of using high amylose maize starch granules as a delivery system for probiotic bacteria has been investigated Bifidobacterium spp. LaftiTM 8B and 13B which were isolated from healthy human. cells able to adhere the amylomaize also hydrolyse during growth. Initially, in vitro studies carried out by studying survival strains when exposed pH 2·3, 3·5 6·5 well 0·03 0·05% w/v bile acids. Both grown either absence or presence granules, then mixed with acidic buffers acid solutions....
ABSTRACT It has been well established that a certain amount of ingested starch can escape digestion in the human small intestine and consequently enters large intestine, where it may serve as carbon source for bacterial fermentation. Thirty-eight types colonic bacteria were screened their capacity to utilize soluble starch, gelatinized amylopectin maize high-amylose granules by measuring clear zones on agar plates. The six cultures which produced starch- containing plates selected further...
Aims: To study the effects of amylomaize starch and modified (carboxymethylated acetylated) starches on composition colonic bacteria production volatile fatty acids, in mice. Methods Results: Balb/c mice were fed with experimental diets containing various amount starches. Colonic bacterial populations short‐chain acids monitored. Results showed that increases indigenous bifidobacteria detected all tested; however, highest numbers observed group 40% unmodified starch. The type influenced...
Objective Evidence indicates that multistrain probiotics benefit preterm infants more than single-strain (SS) probiotics. We assessed the effects of SS versus triple-strain (TS) probiotic supplementation (PS) in extremely (EP) infants. Design EP (gestational age (GA) <28 weeks) were randomly allocated to TS or probiotic, assuring blinding. Reference (REF) group was placebo arm our previous trial. PS commenced with feeds and continued until 37 weeks’ corrected GA. Primary outcome time full...
Ileal mucus and epithelial cells were isolated from newborn piglets that had never been fed 35-day-old unweaned piglets. Both piglet preparations supported growth of Escherichia coli Bd 1107/75 08, a K88-fimbriated porcine enterotoxigenic strain, equally well (i.e., generation times 28 min observed in both cases). Adhesion E. 08 to ileal was, at most, 2 the same strain cells; however, adhesion was 16 mucus. The receptor K88 specific, since it could be removed by purified K88ab fimbriae....
The aim of this study was to characterize the Escherichia coli K88-specific receptors in mucus from small intestines 35-day-old piglets with isogenic strains E. K-12(pMK005) (K88+) and K-12(pMK002) (K88-). These differed only that latter one cannot produce intact K88 fimbriae because a deletion gene coding for major fimbrial subunit. Adhesion studied by incubating 3H-labeled bacteria crude mucus, pronase-treated whole fractionated gel filtration, delipidated or extracted lipids immobilized...