A5081399822- Parasites and Host Interactions
- Helminth infection and control
- Parasitic infections in humans and animals
- Research on Leishmaniasis Studies
- Parasitic Infections and Diagnostics
- Parasite Biology and Host Interactions
- Brucella: diagnosis, epidemiology, treatment
- Diagnosis and treatment of tuberculosis
- Toxoplasma gondii Research Studies
- Iron Metabolism and Disorders
- Polyomavirus and related diseases
- Trace Elements in Health
- Congenital Anomalies and Fetal Surgery
- Viral Infections and Vectors
- Prion Diseases and Protein Misfolding
- Vector-borne infectious diseases
- Health Education and Validation
- Tuberculosis Research and Epidemiology
- Health, Nursing, Elderly Care
- Pancreatitis Pathology and Treatment
- Bacteriophages and microbial interactions
- Science and Education Research
- Sex work and related issues
- Invertebrate Immune Response Mechanisms
- Studies on Chitinases and Chitosanases
Universidade Federal de Uberlândia
2014-2025
IgG avidity assays have been developed for several parasitic diseases although there are no researches focused in strongyloidiasis diagnosis. Definitive diagnosis of is based on the presence Strongyloides larvae stool, but majority cases involve low and irregular larval output. While limitations serological well known, characteristics persons who misdiagnosed negative coproparasitological tests little explored. The aim present study was to evaluate use detect patients with active...
Glycosylated components from Strongyloides have an important role in parasite establishment and host recognition of these substances. Considering the sugar-binding capacity lectins, such as concanavalin-A (Con-A), IgG IgA detection serum samples strongyloidiasis patients was tested using different antigenic preparations. The total saline extract (SE) venezuelensis filariform larvae fractionated Con-A column to obtain unbound (Con-A UF) bound BF) fractions. Sensitivity (Se), specificity (Sp),...
Background Strongyloidiasis, a human intestinal infection caused by the nematode Strongyloides stercoralis, is frequently underdiagnosed and although its high prevalence still neglected parasitic disease because conventional diagnostic tests based on parasitological examination (presence of larvae in stool) are not sufficiently sensitive due to low load irregular larval output. There an urgent need improve assays, especially for immunocompromised patients with as consequence self-infection...
The aim of this study was to detect levels IgG and IgA by enzyme-linked immunosorbent assay (ELISA) using alkaline extracts larvae, adult female worms, eggs Strongyloides venezuelensis as antigen. One hundred twenty serum samples divided into 3 groups were analysed: group I (40 strongyloidiasis patients), II patients with other parasitic infections), III healthy subjects). Statistical variations analyzed analysis variance. There a significant statistical difference (P < 0.001) in the...
Strongyloidiasis is one of the major intestinal infections in humans, and a neglected tropical disease whose diagnosis still poses challenge. We hypothesized that diagnostic tests based on short peptides containing epitopes may represent promising strategy to improve strongyloidiasis detection due reduced cross-reactivity higher sensitivity. Our aim was evaluate two synthetic selected by phage display (C10 D3) as potential tools for serodiagnosis strongyloidiasis, predict their putative...
Strongyloides stercoralis causes chronic asymptomatic infections in immunocompetent human hosts and systemic invasion immunocompromised patients, developing into a fatal hyperinfection syndrome. IgA IgG detection saliva serum paired samples were tested using total saline extract from venezuelensis (SE(*)) its detergent phase (D) extracted with Triton X-114. Saliva obtained from: 25 patients confirmed strongyloidiasis; other parasitoses 20 apparently healthy individuals. Sensitivity,...
SUMMARY The aim of this study was to fractionate and partially characterize the antigenic extract filariform larvae Strongyloides venezuelensis in ion-exchange resin diethylaminoethyl sepharose (DEAE), obtain fractions potentially applicable immunoassays. Somatic antigen (SA) its DEAE S1 S2 - which interacted with were evaluated by 1-dimensional electrophoresis protein profiles. SA tested serum samples for IgG detection ELISA. Serum ( n = 155) analysed: 50 from strongyloidiasis patients...
We have shown previously that detection of circulating antibodies against mimotopes selected by phage display were useful in neurocysticercosis diagnosis. However, antigens may also be patients' clinical follow-up. Therefore, we aimed to select novel combinatorial antibodies, single-chain variable fragment (scFv), which can used for specific with pre-defined affinity and specificity without prior immunization. A scFv antibody library was Taenia solium displayed on phages coupled beads total...
SUMMARY There is an increasing interest in improving neurocysticercosis (NCC) diagnosis through the search of new and alternative antigenic sources, as those obtained from heterologous antigens. The aim this study was to obtain potential biomarkers for NCC after gel filtration chromatography [gel fraction (GFF)] total saline extract (SE) Taenia saginata metacestodes, followed by protein identification application immunodiagnostic. SE GFF proteic profiles were characterized electrophoresis,...
Objective. To understand how congenital toxoplasmosis (CT) diagnosis has evolved over the years, we performed a systematic review and meta-analysis to summarize kind of analysis that been employed for CT diagnosis. Methods. PubMed Lilacs databases were used in order access several samples. Our search combined following combining terms: “congenital toxoplasmosis” or “gestational “diagnosis” “blood,” “serum,” “amniotic fluid,” “placenta,” “colostrum.” We extracted data on true positive,...
The aim of this study was to fractionate and partially characterize fractions obtained from the total saline extract (SE) Taenia saginata metacestodes after ion-exchange procedure in carboxymethyl sepharose (CM) diethylaminoethyl (DEAE) resins, as a source antigenic markers applicable immunodiagnosis neurocysticercosis (NCC). For IgG detection by enzyme-linked immunosorbent assay (ELISA) immunoblotting, 140 serum samples were analyzed: 45 patients with NCC (G1), 50 other parasitic infections...
This study evaluates the inclusion of IgG avidity index in ELISA to detect anti-Strongyloides stercoralis IgG. The revealed 70% specificity. With screening AI, specificity increased 80%. complemented traditional by eliminating some suspected or false positive cases.
Abstract Aims To describe an anti‐ Strongyloides IgA, IgG and immune complex antibody response profile in patients with pulmonary tuberculosis. Methods Results Saliva serum samples were collected from 100 individuals: group I, 50 apparently healthy individuals; II, tuberculosis patients. The detection carried out via ELISA immunoenzymatic test. Optical density medians saliva of IgA (median 7.21) IgG‐IC 4.95) significantly higher compared to control individuals 3.93 2.38). Conclusion This...
A estrongiloidiase humana, causada pelo geohelminto Strongyloides stercoralis , e descrita como doenca negligenciada. Milhoes de pessoas estao infectadas em todo o mundo, porem ha uma subestimativa dos acometidos, devido a baixa sensibilidade metodos diagnostico da liberacao irregular das larvas nas fezes. pode ser assintomatica, mas pacientes imunocomprometidos levar ao obito. Considerando cronicidade, potencial mortalidade as dificuldades diagnostico, varios esforcos tem sido feitos para...
Strongyloides stercoralis , nematodeo parasito intestinal negligenciado, infecta milhoes de pessoas. Os testes sorodiagnosticos apresentam os parâmetros reduzidos devido a complexidade dos preparados antigenicos. Este estudo avaliou fracoes antigenicas larvas filarioides S. venezuelensis . O extrato salino (ES) heterologo foi fracionado por cromatografia em resina gel filtracao (Sephacryl S-100). perfil proteico caracterizado eletroforese 1D e para definicao das (F1, F2, F3 F4). ES foram...
A neurocisticercose (NC), causada pelas formas metacestodeas de Taenia solium , e listada pela Organizacao Mundial da Saude como uma zoonose endemica negligenciada, alem ser das principais causas convulsoes epilepsia. O diagnostico clinico doenca dificil variedade sintomas o imunodiagnostico limitado reatividade cruzada. Pesquisas tem sido realizadas buscando antigenos altamente sensiveis especificos com aplicabilidade em plataformas diagnosticas baixo custo ELISA ( enzyme linked...