A5103068129- Zebrafish Biomedical Research Applications
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- Nuclear Receptors and Signaling
- Pregnancy and preeclampsia studies
- CRISPR and Genetic Engineering
- Immune cells in cancer
- Remote Sensing and Land Use
- Machine Learning in Materials Science
- Land Use and Ecosystem Services
- Genomics and Chromatin Dynamics
- Perovskite Materials and Applications
- RNA Interference and Gene Delivery
- Environmental Changes in China
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Olfactory and Sensory Function Studies
- Advanced biosensing and bioanalysis techniques
- Hepatitis B Virus Studies
- Reinforcement Learning in Robotics
- Electrochemical sensors and biosensors
- Renal and related cancers
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Circular RNAs in diseases
University of Edinburgh
2006-2023
Beijing Jiaotong University
2022-2023
MRC Centre for Regenerative Medicine
2011-2023
Corteva (United States)
2020
Medical Research Council
2011-2017
Care Resource
2003-2004
Pioneer Hi-Bred
2003
Sanford Burnham Prebys Medical Discovery Institute
1996
Discovery Institute
1996
In the developing mouse embryo first definitive(transplantable-into-the-adult) haematopoietic stem cells/long-term repopulating units (HSC/RUs) emerge in AGM region and umbilical vessels on 10-11 days post coitum (d.p.c.). Here, by limiting dilution analysis, we anatomically map development of definitive HSC/RUs different embryonic tissues during early colonisation liver. We show that day 12 p.c. contains about 66 (53 liver, 13 other tissues), whereas previous total number entire conceptus...
The aorta-gonad-mesonephros region plays an important role in hematopoietic stem cell (HSC) development during mouse embryogenesis. vascular endothelial cadherin⁺ CD45⁺ (VE-cad⁺CD45⁺) population contains the major type of immature pre-HSCs capable developing into long-term repopulating definitive HSCs. In this study, we developed a new coaggregation culture system, which supports maturation novel CD45-negative (VE-cad⁺CD45⁻CD41⁺) appearance these precedes VE-cad⁺CD45⁺ (termed here I and II...
During gastrulation, epiblast cells are pluripotent and their fate is thought to be constrained principally by position. Cell progressively restricted localised signalling cues from areas including the primitive streak. However, it unknown whether this restriction accompanies, at individual cell level, a reduction in potency. Investigation of these early transition events vitro possible via use stem (EpiSCs), self-renewing lines equivalent postimplantation epiblast. Strikingly, mouse EpiSCs...
Abstract We have generated embryonic stem (ES) cells and transgenic mice with green fluorescent protein (GFP) inserted into the Pitx3 locus via homologous recombination. In central nervous system, ‐directed GFP was visualized in dopaminergic (DA) neurons substantia nigra ventral tegmental area. Live primary DA can be isolated by fluorescence‐activated cell sorting from these mouse embryos. culture, –GFP is coexpressed a proportion of ES‐derived neurons. Furthermore, ES cell‐derived...
One day prior to mass emergence of haematopoietic stem cells (HSCs) in the foetal liver at E12.5, embryo contains only a few definitive HSCs. It is thought that burst HSC activity underpinned by rapid maturation immature embryonic precursors HSCs, termed pre-HSCs. However, because pre-HSCs are not detectable direct transplantations into adult irradiated recipients, size and growth this population, which represents rudiment system, remains uncertain. Using novel quantitative assay, we...
During embryonic development, adult haematopoietic stem cells (HSCs) emerge preferentially in the ventral domain of aorta aorta-gonad-mesonephros (AGM) region. Several signalling pathways such as Notch, Wnt, Shh and RA are implicated this process, yet how these interact to regulate emergence HSCs has not previously been described mammals. Using a combination ex vivo approaches, we report here that stage-specific reciprocal dorso-ventral inductive interactions lateral input from urogenital...
During development, hematopoietic stem cells (HSCs) emerge in the aorta-gonad-mesonephros (AGM) region through a process of multi-step maturation and expansion. While proliferation adult HSCs is implicated balance between self-renewal differentiation, very little known about status nascent AGM region. Using Fucci reporter mice that enable vivo visualization cell-cycle status, we detect increased during pre-HSC expansion followed by slowing down cycling once start to acquire definitive HSC...
Haematopoiesis in adult animals is maintained by haematopoietic stem cells (HSCs), which self-renew and can give rise to all blood cell lineages. The AGM region an important intra-embryonic site of HSC development a wealth evidence indicates that HSCs emerge from the endothelium embryonic dorsal aorta extra-embryonic large arteries. This, however, stepwise process occurs through sequential upregulation CD41 CD45 followed emergence fully functional definitive HSCs. Although largely...
Abstract Induced pluripotent stem cell (iPSC) reprogramming is inefficient and understanding the molecular mechanisms underlying this inefficiency holds key to successfully control cellular identity. Here, we report 24 roadblock genes identified by CRISPR/Cas9-mediated genome-wide knockout (KO) screening. Of these, depletion of predicted KRAB zinc finger protein (KRAB-ZFP) Zfp266 strongly consistently enhances murine iPSC generation in several settings, emerging as most robust roadblock. We...
The integration of human and artificial intelligence represents a scientific opportunity to advance our understanding information processing, as each system offers unique computational insights that can enhance inform the other. synthesis cognitive principles with has potential produce more interpretable functionally aligned models, while simultaneously providing formal framework for investigating neural mechanisms underlying perception, learning, decision-making through systematic model...
Hematopoietic stem cells (HSCs) develop in the embryonic aorta-gonad-mesonephros (AGM) region and subsequently relocate to fetal liver. Runx1 transcription factor is essential for HSC development, but largely dispensable adult HSCs. Here, we studied tamoxifen-inducible inactivation vivo. Induction at pre-liver stages (up day 10.5) reduced erythromyeloid progenitor numbers, surprisingly did not block appearance of Runx1-null HSCs By contrast, ex vivo analysis showed an absolute dependency...
Abstract The midbrain dopaminergic system, which consists of neurons the substantia nigra and ventral tegmental area, is a subject intense interest, since loss from results in motor disorders characteristic Parkinson's disease. We have generated knock‐in reporter mouse line with tau‐lacZ fusion gene inserted into Pitx3 locus via homologous recombination. This approach permitted visualisation specific axonal tracts both area phenotypically normal heterozygous Pitx3‐taulacZ brain tissues,...
Abstract Among the various abiotic stresses, water and nitrogen are major stress factors that limit crop productivity worldwide. Since leaf nutrients remobilization during senescence might impact response to in crops, we undertook a forward screen of Mutator ‐active approach identify premature loci maize. A mutant line isolated from cross between Pioneer Brand elite public material, designated senescence2 ( pre2 ), expressed flower initiation. The Pre2 gene encodes PHYTOCHROME‐DEPENDENT...
This study was conducted to develop an appropriate assessment technique define impact of mountain-desert-oasis ecosystem on net primary productivity (NPP) in northern foothills Tianshan Mountains. Geographic Information System (GIS) used estimate land use/land cover the mountain, desert and oasis zones. An ecological process model NPP by using data entirely derived from satellite. The results show that landscape heterogeneity important factor affect values ecosystem. Simulated indicated a...
Heat, precipitation and humidity affect vegetation types their distribution. However, degree of effects is highly spatial temporal dependent. When we study the major factors which cover, need define a specific region time period. In order to land cover change in Xinjiang probe its driving force from 1992 2000, analyzed sensitivity land-cover climate using Remote Sensing (RS) Geographic Information System (GIS) with multi-temporal NOAA/AVHRR NDVI images. Major considered this were...
Microarray technology has become increasingly useful in measuring expression levels of a large number genes and part repertoire functional genomic tools. We describe the methods cDNA microarray preparation, use, data collection, initial processing. The fragments are first prepared by polymerase chain reaction (PCR), then attached to solid substrate, such as chemically treated glass slide. Robotic machines spot cloned samples miniaturized gridded pattern, so that nanoliter amounts tens...