A5103147633- Nanocomposite Films for Food Packaging
- Advanced Cellulose Research Studies
- Axon Guidance and Neuronal Signaling
- Meat and Animal Product Quality
- Calpain Protease Function and Regulation
- Identification and Quantification in Food
- Protein Hydrolysis and Bioactive Peptides
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Spectroscopy and Chemometric Analyses
- Nerve injury and regeneration
- Electrospun Nanofibers in Biomedical Applications
- Adsorption and biosorption for pollutant removal
- Thermochemical Biomass Conversion Processes
- Neuroinflammation and Neurodegeneration Mechanisms
- Subcritical and Supercritical Water Processes
- Isotope Analysis in Ecology
- Chromium effects and bioremediation
- Insect Utilization and Effects
- Coal and Its By-products
- Curcumin's Biomedical Applications
- Hippo pathway signaling and YAP/TAZ
- Dye analysis and toxicity
- Autophagy in Disease and Therapy
- Lysosomal Storage Disorders Research
China Agricultural University
2022-2025
Tsinghua University
2025
National University of Singapore
2023-2024
Northeast Agricultural University
2018
Western University of Health Sciences
2009-2010
Kobe University
2003-2006
Calpain is a calcium-dependent protease that plays significant role in synaptic plasticity, cell motility, and neurodegeneration. Two major calpain isoforms are present brain, with μ-calpain (calpain1) requiring micromolar calcium concentrations for activation m-calpain (calpain2) needing millimolar concentrations. Recent studies fibroblasts indicate epidermal growth factor (EGF) can activate independently of via mitogen-activated protein kinase (MAPK)-mediated phosphorylation. In neurons,...
17-Beta-estradiol (E2) is a steroid hormone involved in numerous brain functions. E2 regulates synaptic plasticity part by enhancing NMDA receptor function and spine density the hippocampus, resulting increased long-term potentiation facilitation of learning memory. As calcium-dependent neutral protease, calpain, also these processes, we tested whether could activate calpain examined functional consequences E2-mediated activation hippocampus. Calpain activity was analyzed fluorescence...
Microwave power as directly controlled parameter determines the absorption of microwave energy inside extraction vial and yield objective component in microwave-assisted (MAE). The aim this study was to elucidate effects powers on anthocyanin from blueberry ( Vaccinium spp.) powder based extracts under MAE. Rising have little effect distribution vial, but increase its temperature. simulation results indicated that strength electrical field tends decay trend along with irradiation; however,...
Abstract BACKGROUND In our previous study, we successfully identified five peptides from wheat gluten: Ala‐Pro‐Ser‐Tyr (APSY), Leu‐Tyr (LY), Pro‐Tyr (PY), Arg‐Gly‐Gly‐Tyr (RGGY) and Tyr‐Gln (YQ). Molecular docking molecular dynamics simulation methods were employed to investigate the interaction between these antioxidant Kelch‐like ECH‐associated protein 1 (Keap1 protein), revealing mechanism of their non‐competitive binding. addition, total capacity was determined using...
The transcription factor p53 suppresses tumorgenesis by regulating cell proliferation and migration. We investigated whether could also control motility in postmitotic neurons. isoforms recognized phospho-p53-specific (at Ser-15) or “mutant” conformation-specific antibodies were highly specifically expressed axons axonal growth cones primary hippocampal Inhibition of function inhibitors, small interfering RNAs, dominant-negative forms, induced cone collapse, whereas overexpression led to...
Polyglutamine diseases are inherited neurodegenerative caused by the expanded polyglutamine proteins (polyQs). We have identified a novel guanosine triphosphatase (GTPase) named CRAG that contains nuclear localization signal (NLS) sequence and forms inclusions in response to stress. After ultraviolet irradiation, interacted with induced an enlarged ring-like structure of promyelocytic leukemia protein (PML) body GTPase-dependent manner. Reactive oxygen species (ROS) generated polyQ...
Collapsin response mediator proteins (CRMPs) have been implicated in signaling of axonal guidance, including semaphorins. We previously identified a unique member this gene family, CRMP-associated molecule CRAM (CRMP-5), which is phylogenetically divergent from the other four CRMPs. In study, we examined distribution and function developing neurons. Immunohistochemical analysis showed accumulation filopodia growth cones. Experiments using cytochalasin D indicated that filopodial localization...
Neurite outgrowth represents a critical stage in the correct development of neuronal circuitries, and is dependent on complex regulation actin filament microtubule dynamics by intrinsic as well extrinsic signals. Previous studies have implicated tumor suppressor factor, p53, axonal through nontranscriptional effect involving local Rho kinase signaling pathway that controls these dynamics. In present study, we first showed semaphorin 3A-induced growth cone collapse cultured hippocampal...
We have previously demonstrated that Fes/Fps (Fes) tyrosine kinase is involved in Semaphorin3A-mediated signaling. Here we report a role for Fes microtubule dynamics. A fibrous formation of was observed kinase-dependent manner, which associated with microtubules and functionally correlated bundling. Microtubule regeneration assays revealed aggregates colocalized gamma-tubulin at nucleation sites Fes/CIP4 homology (FCH) domain-dependent manner expression FCH domain-deleted mutants blocked...
The annual discharge (6–7 million tons per year) of Chinese medicine industry waste (CMIW) is large and harmful. CMIW with a high moisture content can be effectively treated by hydrothermal carbonization (HTC) technology. Compared CMIW, the volume number pores prepared hydrochar increased significantly after alkali etching (AE), they had abundant oxygen-containing functional groups. These properties provide physical chemical adsorption sites, improving adsorbent activity alkaline (AE-CMIW...
Perturbation of lipid metabolism, especially cholesterol homeostasis, can be catastrophic to mammalian brain, as it has the highest level in body. This notion is best illustrated by severe progressive neurodegeneration Niemann-Pick Type C (NPC) disease, one lysosomal storage diseases, caused mutations NPC1 or NPC2 gene. In this study, we found that growth cone collapse induced genetic pharmacological disruption egress from late endosomes/lysosomes was directly related a decrease axonal and...