A5007689920- Enzyme Catalysis and Immobilization
- Nuclear physics research studies
- Astronomical and nuclear sciences
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Amino Acid Enzymes and Metabolism
- Acoustic Wave Resonator Technologies
- Carbohydrate Chemistry and Synthesis
- Nuclear Physics and Applications
- Enzyme Structure and Function
- Quantum Chromodynamics and Particle Interactions
- Atomic and Molecular Physics
- Ecology and Conservation Studies
- Chemical Synthesis and Analysis
- HVDC Systems and Fault Protection
- Biofuel production and bioconversion
- Allelopathy and phytotoxic interactions
- Pancreatic function and diabetes
- Plant Parasitism and Resistance
- Protein Hydrolysis and Bioactive Peptides
- Advanced Chemical Physics Studies
- Biochemical and Molecular Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- High-Voltage Power Transmission Systems
- Ferroelectric and Piezoelectric Materials
East China University of Science and Technology
2015-2025
China National Research Institute of Food and Fermentation
2024-2025
Guizhou University
2008-2024
Zhengzhou University
2011-2024
Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences
2022-2023
University of Electronic Science and Technology of China
2008-2023
Changsha University of Science and Technology
2021-2022
Czech Academy of Sciences, Institute of Biotechnology
2017-2021
State Council of the People's Republic of China
2020-2021
Xidian University
2018
Enzymatic asymmetric synthesis of l-phenylglycine by amino acid dehydrogenases has potential for industrial applications; however, this is hindered their low catalytic efficiency toward high-concentration substrates. We identified and characterized a novel leucine dehydrogenase (MsLeuDH) with high benzoylformic via directed metagenomic approaches. Further, we obtained triple-point mutant MsLeuDH-EER (D332E/G333E/L334R) improved stability through the rational design distal loop 13. A...
Marine mud is an abundant and largely unexplored source of enzymes with unique properties that may be useful for industrial biotechnological purposes. However, since most microbes cannot cultured in the laboratory, a cultivation-independent metagenomic approach would advantageous identification novel enzymes. Therefore, objective screening lipolytic enzymes, library was constructed using total genomic DNA extracted from marine mud. Based on functional heterologous expression, 34 clones...
α-Amylase plays a pivotal role in broad range of industrial processes. To meet increasing demands biocatalytic tasks, considerable efforts have been made to isolate enzymes produced by extremophiles. However, the relevant data α-amylases from cold-adapted fungi are still insufficient. In addition, bread quality presents particular interest due its high consummation. Thus developing amylases improve textural properties could combine health benefits with good sensory properties. Furthermore,...
Abstract BACKGROUND In our previous study, we successfully identified five peptides from wheat gluten: Ala‐Pro‐Ser‐Tyr (APSY), Leu‐Tyr (LY), Pro‐Tyr (PY), Arg‐Gly‐Gly‐Tyr (RGGY) and Tyr‐Gln (YQ). Molecular docking molecular dynamics simulation methods were employed to investigate the interaction between these antioxidant Kelch‐like ECH‐associated protein 1 (Keap1 protein), revealing mechanism of their non‐competitive binding. addition, total capacity was determined using...
Abstract Background A nitrilase-mediated pathway has significant advantages in the production of optically pure (R)-(−)-mandelic acid. However, unwanted byproduct, low enantioselectivity, and specific activity reduce its value practical applications. An ideal nitrilase that can efficiently hydrolyze mandelonitrile to acid without byproduct is needed. Results novel (BCJ2315) was discovered from Burkholderia cenocepacia J2315 through phylogeny-based enzymatic substrate specificity prediction...
Abstract ω‐Transaminases (ω‐TAs) show considerable potential for the synthesis of chiral amines. However, their low catalytic efficiency towards bulky substrates limits application, and complicated mechanisms prevent precise enzyme design. Herein, we address this challenge using a mechanism‐guided computational design strategy by reprograming transition ground states in key reaction steps. The common features among three high‐energy‐barrier steps responsible were revealed quantum mechanics...
(R)-(−)-Mandelic acid is an important chiral building block that widely used in pharmacy and the production of fine chemicals. A more advanced method for obtaining (R)-(−)-mandelic direct hydrolysis corresponding racemic mandelonitrile. In order to develop a cost-effective process, highly efficient enantioselective nitrilase BCJ2315 from Burkholderia cenocepacia J2315 was biotransformation mandelonitrile acid. The recombinant Escherichia coli M15/BCJ2315 showed high substrate tolerance could...
Bioreductions catalyzed by alcohol dehydrogenases (ADHs) play an important role in the synthesis of chiral alcohols. However, ethyl (S)-4-chloro-3-hydroxybutyrate [(S)-CHBE], drug intermediate, has significant challenges concerning high substrate or product inhibition toward ADHs, which complicates its production. Herein, we evaluated a novel ADH, SmADH31, obtained from Stenotrophomonas maltophilia genome, can tolerate extremely concentrations (6 M) both and product. The coexpression SmADH31...
High-accuracy mass measurements of neutron-deficient Yb isotopes have been performed at TRIUMF using TITAN's multiple-reflection time-of-flight spectrometer (MR-TOF-MS). For the first time, an MR-TOF-MS was used on line simultaneously as isobar separator and a spectrometer, extending to two further away from stability than otherwise possible. The ground state masses $^{150,153}\mathrm{Yb}$ excitation energy $^{151}{\mathrm{Yb}}^{m}$ were measured for time. As result, persistence $N=82$ shell...
Abstract BACKGROUND There are more and Alzheimer's patients. The formation of plaques A β 1‐42 in the brain is one main causes disease. Corn oligopeptides have natural antioxidant effects. It aimed to develop functional corn prevent disease through antioxidation. METHODS According previous laboratory studies, peptides were screened by biological activity score ADMET prediction, molecular docking technology was used screen that had high binding energy with . protective effects selected...
The magnetic chitosan nanocomposites have been studied intensively and used practically in various biomedical biological applications including enzyme immobilization. However, the loading capacity remained activity of immobilized based on existing approaches are not satisfied. Simpler more effective immobilization strategies needed. Here we report a simple catechol modified protocol for preparing novel catechol-chitosan (CCS) - iron oxide nanoparticles (IONPs) composites carrying adhesive...
Quadrupole and octupole stiffnesses in the ground states of even-even $^{112\ensuremath{-}150}\mathrm{Ba}$ isotopes have been systematically investigated by means potential-energy-surface calculations. The calculations are carried out both $({\ensuremath{\beta}}_{2},\ensuremath{\gamma},{\ensuremath{\beta}}_{4})$...
Elicitation is a well-known strategy for increasing the accumulation of secondary metabolites in callus cultures. In this study, induction system Saxifraga stolonifera Meerb. was developed, and elicitors were used to enhance metabolite production. Tender leaves S. as explants vitro culture. We examined characteristics its response various by assessing sterilization conditions explants, varying hormone ratios, determining optimal concentrations salicylic acid (SA), Sodium nitroprusside (SNP)...
The nitrilase-mediated pathway has significant advantages in the production of optically pure aromatic α-hydroxy carboxylic acids. However, low enantioselectivity and activity are observed on hydrolyzing o-chloromandelonitrile to produce (R)-o-chloromandelic acid. In present study, a protein engineering approach was successfully used enhance performance nitrilase obtained from Burkholderia cenocepacia strain J2315 (BCJ2315) o-chloromandelonitrile. Four hot spots (T49, I113, Y199, T310)...
The investigation of molecular mechanisms involved in polysaccharides and saponin metabolism is critical for genetic engineering Polygonatum cyrtonema Hua to raise major active ingredient content. Up now, the transcript sequences are available different tissues P. cyrtonema, a wide range scanning about temporal at ages' rhizomes was still absent cyrtonema.Transcriptome sequencing ages performed. Sixty-two thousand six hundred thirty-five unigenes were generated by assembling transcripts from...
This study aimed to gain an understanding of the possible function NACs by examining their physicochemical properties, structure, chromosomal location, and expression. Being a family plant-specific transcription factors, NAC (petunia no apical meristem Arabidopsis thaliana ATAF1, ATAF2, CUC2) is involved in plant growth development. None genes has been reported Akebia trifoliata (Thunb.) Koidz (A. trifoliata). In this study, we identified 101 proteins (AktNACs) A. genome bioinformatic...