A5068803622- Chronic Kidney Disease and Diabetes
- Endoplasmic Reticulum Stress and Disease
- Renal Diseases and Glomerulopathies
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Retinal and Macular Surgery
- Glycosylation and Glycoproteins Research
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Biomedical Research and Pathophysiology
- Renal and related cancers
- Caveolin-1 and cellular processes
- Chemokine receptors and signaling
- Extracellular vesicles in disease
- Pregnancy and preeclampsia studies
- Cellular transport and secretion
- Renal and Vascular Pathologies
- Retinal Diseases and Treatments
- Salivary Gland Tumors Diagnosis and Treatment
- Reproductive Biology and Fertility
- Cellular Mechanics and Interactions
University of Pittsburgh
2022-2024
Magee-Womens Research Institute
2022
Slippery Rock University
2020
University of Wisconsin–Whitewater
2017
Reproduction is an energy-intensive process requiring systemic coordination. However, the inter-organ signaling mechanisms that relay nutrient status to modulate reproductive output are poorly understood. Here, we use Drosophila melanogaster as a model establish integrated stress response (ISR) transcription factor, Atf4, fat tissue metabolic sensor instructs oogenesis. We demonstrate Atf4 regulates lipase activity mediate yolk lipoprotein synthesis in body. Depletion of body also blunts...
Tyrosine sulfation, a post-translational modification found on many chemokine receptors, typically increases receptor affinity for the ligand. A previous bioinformatics analysis suggested that sulfotyrosine (sY)-binding site surface of CXCL12 may be conserved throughout family. However, extent to which tyrosine sulfation contributes binding has been examined in only few instances. Computational solvent mapping correctly identified sulfotyrosine-binding sites and CCL21 detected by nuclear...
Proximal tubule (PT) cells maintain a high-capacity apical endocytic pathway to recover essentially all proteins that escape the glomerular filtration barrier. The multiligand receptors megalin and cubilin play pivotal roles in uptake of normally filtered PT but also contribute nephrotoxic drugs, including aminoglycosides. We previously demonstrated opossum kidney (OK) cultured under continuous fluid shear stress (FSS) are superior static conditions recapitulating essential functional...
Kidney proximal tubule (PT) cells are specialized for effcient apical uptake of albumin and other proteins that escape the glomerular filtration barrier. The endocytic pathway is uniquely organized to maintain highly dynamic retrieval these via binding multiligand receptors megalin cubilin. Ultrastructural studies following fate internalized markers have provided a temporal model delivery receptor-bound ligands fluid phase lysosomes. In this model, internalization from membrane occurs...
Subcellular organelles communicate with one another and the plasma membrane (PM). A wide variety of contact sites (MCS) tether subcellular structures to mediate inter-organelle communication exchange. The nature, distribution function within segment-specific architecture renal epithelial cells has only just begun be elucidated. We have used advanced imaging techniques, including Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) machine learning-based segmentation, create a...
Proximal tubule (PT) cells maintain a high-capacity apical endocytic pathway to recover essentially all proteins that escape the glomerular filtration barrier. The multi ligand receptors megalin and cubilin play pivotal roles in uptake of normally filtered PT but also contribute nephrotoxic drugs, including aminoglycosides. We previously demonstrated opossum kidney (OK) cultured under continuous fluid shear stress (FSS) are superior static conditions recapitulating essential functional properties
Reproduction is an energy-intensive process requiring systemic coordination. However, the inter-organ signaling mechanisms that relay nutrient status to modulate reproductive output are poorly understood. Here, we use
Reproduction is an energy-intensive process requiring systemic coordination. However, the inter-organ signaling mechanisms that relay nutrient status to modulate reproductive output are poorly understood. Here, we use Drosophila melanogaster as a model establish Integrated Stress response (ISR) transcription factor, Atf4, fat tissue metabolic sensor which instructs oogenesis. We demonstrate Atf4 regulates lipase Brummer mediate yolk lipoprotein synthesis in body. Depletion of body also...
The proximal tubule (PT) of the kidney is comprised cells that are uniquely specialized for efficient apical uptake albumin and other proteins escape glomerular filtration barrier. These bind to multiligand receptors megalin cubilin internalized via clathrin-dependent endocytosis into early endosomes rapidly mature larger vacuoles (AVs). Dissociated ligands delivered from AVs lysosomes degradation, while collected dense tubules recycling cell surface. When grown under continuous fluid shear...
Lackner, Emily; Shipman, Katherine E.; Cowan, Isabella; Long, Kimberly R.; Weisz, Ora A. Author Information
Heat shock factor (HSF) is a transcription that activated in response to an accumulation of misfolded proteins the cytoplasm. In contrast, unfolded protein (UPR) transcriptional pathway responds endoplasmic reticulum. Both pathways, when by proteins, result helps cell survive making factors help sequester and destroy proteins. Using aseptic techniques, Saccharomyces cerevisiae with UPR or HSF Beta‐galactosidase reporter plasmids were grown tested various doses 4 different chemical stressors...