A5022513303- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Sports Performance and Training
- Caveolin-1 and cellular processes
- Sports injuries and prevention
- Lipoproteins and Cardiovascular Health
- Cardiovascular and exercise physiology
- Exercise and Physiological Responses
- Adipose Tissue and Metabolism
- Hemodynamic Monitoring and Therapy
- Sulfur Compounds in Biology
- Receptor Mechanisms and Signaling
- Cardiovascular Effects of Exercise
- Cardiac Imaging and Diagnostics
- Thermoregulation and physiological responses
- Venous Thromboembolism Diagnosis and Management
- Cardiomyopathy and Myosin Studies
- Cardiac, Anesthesia and Surgical Outcomes
- Neurobiology and Insect Physiology Research
- Signaling Pathways in Disease
- Ion Channels and Receptors
- Atrial Fibrillation Management and Outcomes
- Frailty in Older Adults
- Muscle and Compartmental Disorders
- Amoebic Infections and Treatments
Hairmyres Hospital
2025
University of Huddersfield
2019
University of Leeds
2010-2017
University of Cambridge
2008-2009
Maxim Integrated (United Kingdom)
2009
McMaster University
1980-2003
Kent State University
1990
Deep-vein thrombosis (DVT) and pulmonary embolism (PE) are associated with major morbidity mortality, their burden often extending to longer-term complications such as event recurrence post-thrombotic syndrome (PTS). Few data exist on the overall economic of DVT PE sequelae. A retrospective observational cohort study was conducted determine direct medical costs a or patient across entire continuum care.Administrative claims for patients diagnosis (ICD-9-CM code) possible evidence PTS between...
Inotropy and lusitropy in the ventricular myocyte can be efficiently induced by activation of β1-, but not β2-, adrenoceptors (ARs). Compartmentation β2-AR-derived cAMP-dependent signalling underlies this functional discrepancy. Here we investigate mechanism which caveolae (specialised sarcolemmal invaginations rich cholesterol caveolin-3) contribute to compartmentation adult rat myocyte. Selective β2-ARs (with zinterol/CGP20712A) produced little contractile response control cells pronounced...
β1-Adrenergic receptors (β1ARs) and E-type prostaglandin (EPRs) both produce compartmentalized cAMP responses in cardiac myocytes. The role of cholesterol-dependent lipid rafts producing these was investigated adult rat ventricular β1ARs were found raft non-lipid containing membrane fractions, while EPRs only fractions. Furthermore, β1AR activation enhanced the L-type Ca2+ current, intracellular transient, myocyte shortening, EPR had no effect, consistent with idea that functional are...
The importance of H2S as a physiological signaling molecule continues to develop, and ion channels are emerging major family target proteins through which exerts many actions. purpose the present study was investigate its effects on T-type Ca(2+) channels. Using patch-clamp electrophysiology, we demonstrate that donor, NaHS (10 μM-1 mM) selectively inhibits Cav3.2 heterologously expressed in HEK293 cells, whereas Cav3.1 Cav3.3 were unaffected. sensitivity required presence redox-sensitive...
Intravenous adenosine induces stable myocardial hyperemia for coronary microvascular function testing. Iodinated radiographic contrast media induce transient, submaximal hyperemia. We assessed the feasibility, diagnostic value, and potential cost-effectiveness of contrast-derived indices function. Coronary flow reserve, index resistance, resistance reserve were using a guidewire. Intracoronary bolus thermodilution injections performed at rest, immediately after an 8-mL iohexol, repeated...
Nine membrane-bound mammalian adenylyl cyclases (ACs) have been identified. Type 1 and 8 ACs (AC1 AC8), which are both expressed in the brain stimulated by Ca(2+)/calmodulin (CaM), discrete neuronal functions. Although Ca(2+) sensitivity of AC1 is higher than that AC8, precisely how these two regulated Ca(2+)/CaM remains elusive, basis for their diverse physiological roles quite unknown. Distinct localization CaM binding domains within enzymes may be essential to differential regulation...
Calcium released from the Golgi apparatus may promote receptor trafficking to surface of cardiomyocytes.
The number of people taking statins is increasing across the globe, highlighting importance fully understanding statins' effects on cardiovascular system. beneficial impact extends well beyond regression atherosclerosis to include direct tissues system ('pleiotropic effects'). Pleiotropic cardiac myocyte are often overlooked. Here we consider contribution caveolin protein, whose expression and cellular distribution dependent cholesterol, statin myocyte. Caveolin a structural regulatory...
The ubiquitous Ca(2+)-sensing protein calmodulin (CaM) fulfills its numerous signaling functions through a wide range of modular binding and activation mechanisms. By activating adenylyl cyclases (ACs) 1 8, Ca(2+) acting via impacts on the other major cellular second messenger cAMP. In possessing two CaM-binding domains, 1-5-8-14 motif at N terminus an IQ-like (IQlm) C terminus, AC8 offers particularly sophisticated regulatory possibilities. IQlm has remained unexplored beyond suggestion...
The number of people taking statins is set to increase across the globe due recent changes in prescription guidelines. For example, half US population over 40 now eligible for these drugs, whether they have high serum cholesterol or not. With such development policy comes a stronger need understanding statins' myriad effects. Surprisingly little known about possible direct actions on cardiac myocytes, although claims myocardial toxicity been made. Here we determine impact simvastatin...
New Findings What is the central question of this study? The isolation cardiomyocytes and homogenization myocardium are commonly performed on separate rodent hearts. We asked whether it might be viable to derive cells as well whole muscle (homogenized or cryopreserved) from one organ. main finding its importance? provide examples six diverse experiments that can using tissue samples generated by a modified Langendorff technique. volume power resultant data increased, method may especially...
MacDougall, D.; Tuxon, Sale, Sexton, A.; Moroz, J.; Sutton, J. Author Information
VOLUME 284 (2009) PAGES 4451–4463 In Fig. 1E, the EC50 of AC8 in vitro should be 0.56 ± 0.06, not 0.58.
Shepley, B.; MacDougall, D.; Ciprlano, N.; Sutton, J.; Coates, G.; Tarnopoksky, M. Author Information