A5101842885- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Anesthesia and Sedative Agents
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience of respiration and sleep
- Animal Genetics and Reproduction
- 3D Printing in Biomedical Research
- Angiogenesis and VEGF in Cancer
- Cardiac, Anesthesia and Surgical Outcomes
- Telomeres, Telomerase, and Senescence
- Airway Management and Intubation Techniques
- Obstructive Sleep Apnea Research
- Reproductive Biology and Fertility
- Neonatal Respiratory Health Research
- RNA Interference and Gene Delivery
- Cytokine Signaling Pathways and Interactions
- Selenium in Biological Systems
- Light effects on plants
- Viral Infectious Diseases and Gene Expression in Insects
- Redox biology and oxidative stress
- Developmental Biology and Gene Regulation
- Gene expression and cancer classification
- Hippo pathway signaling and YAP/TAZ
Osaka University
2002-2024
Kumamoto University
1993-2022
Osaka Dental University
2000-2022
RIKEN Center for Computational Science
2003-2017
Japan Science and Technology Agency
2012-2015
Kobe University
2010-2015
Organogenesis (United States)
2014
Tohoku University
2003
National Institute for Basic Biology
2002
Nagoya University
2002
The propagation of embryonic stem (ES) cells in an undifferentiated pluripotent state is dependent on leukemia inhibitory factor (LIF) or related cytokines. These factors act through receptor complexes containing the signal transducer gp130. downstream mechanisms that lead to ES cell self-renewal have not been delineated, however. In this study, chimeric receptors were introduced into cells. Biochemical and functional studies transfected demonstrated a requirement for engagement activation...
Cryptochromes regulate the circadian clock in animals and plants. Humans mice have two cryptochrome ( Cry ) genes. A previous study showed that lacking Cry2 gene had reduced sensitivity to acute light induction of mPer1 suprachiasmatic nucleus (SCN) an intrinsic period 1 hr longer than normal. In this study, Cry1 −/− were generated their clocks analyzed at behavioral molecular levels. Behaviorally, a shorter wild type arrhythmic constant darkness (DD). Biochemically, mRNA SCN was blunted...
Inhibition of glycogen synthase kinase-3 (Gsk3) supports mouse embryonic stem cells (ESCs) by modulating Tcf3, but the critical targets downstream Tcf3 are unclear. We analyzed intersection between genome localization and transcriptome data sets to identify genes repressed Tcf3. Among these, manipulations Esrrb gave distinctive phenotypes in functional assays. Knockdown knockout eliminated response Gsk3 inhibition, causing extinction pluripotency markers loss colony forming capability....
Neuropilins (NP1 and NP2) are vascular endothelial growth factor (VEGF) receptors that mediate developmental tumor angiogenesis. Transgenic mice, in which both NP1 NP2 were targeted −/− ) died utero at E8.5. Their yolk sacs totally avascular. Mice deficient for but heterozygous +/− or also embryonic lethal survived to E10–E10.5. The E10 embryos easier analyze phenotype than the fragile poorly formed 8.5 embryos. phenotypes of these mice very abnormal. sacs, although normal size, lacked...
Abstract Background: Extracellular signal‐regulated kinase 2 (ERK2) has been implicated in cell proliferation, differentiation, and survival. However, its role vivo remains to be determined. Results: Here we show that the targeted disruption of mouse ERK2 gene results embryonic lethality by E11.5 severe abnormality placenta. In these animals, labyrinthine layer placenta is very thin few foetal blood vessels are observed. mutants can rescued transgenic expression ERK2, demonstrating...
Embryonic stem (ES) cells are immortal and pluripotent derived from early mammalian embryos. Transcription factor Oct3/4 is essential for self-renewal of ES mouse development. However, only a few target genes have been identified. In this study, we found that F-box-containing protein Fbx15 was expressed predominantly in undifferentiated cells. Inactivation led to rapid extinction expression. Reporter gene analyses demonstrated cell-specific expression required an 18-bp enhancer element...
The cytotoxicity of reactive oxygen intermediates (ROIs) has been implicated in the destruction pancreatic β cells insulin-dependent diabetes mellitus (IDDM). Thioredoxin (TRX), a redox (reduction/oxidation)-active protein, recently shown to protect from oxidative stress and apoptosis. To elucidate roles development autoimmune vivo, we produced nonobese diabetic transgenic mice that overexpress TRX their cells. In these mice, incidence was markedly reduced, whereas insulitis not prevented....
Somatic development initiates from the epiblast in post-implantation mammalian embryos. Recent establishment of stem cell (EpiSC) lines has opened up new avenues investigation mechanisms that regulate state and initiate lineage-specific somatic development. Here, we investigated role cell-intrinsic core transcriptional regulation during derivation anterior neural plate (ANP) using a mouse EpiSC model. Cells developed EpiSCs one day absence extrinsic signals were found to represent ANP ~E7.5...
Journal Article An Efficient Gene-Trap Method Using Poly A Trap Vectors and Characterization of Events Get access Hitoshi Niwa, Niwa Search for other works by this author on: Oxford Academic PubMed Google Scholar Kimi Araki, Araki 2 2Department Pathology, Centre Medical Universitaire, 1 Rue Michel Servet, CH1211, Geneve 4, Switzerland; 3 Pirat Department Internal medicine, Tottori UniversitySchool Medicine, Yonago, 683 Shigemi Kimura, Kimura Shin-ichi Taniguchi, Taniguchi Shoji Wakasugi,...
Mouse epiblast stem cells (mEpiSCs) are pluripotent derived from epiblasts of postimplantation mouse embryos. Their pluripotency is distinct that embryonic (mESCs) in several cell biological criteria. One the distinctions mEpiSCs contribute either not at all or much lower efficiency to chimeric embryos after blastocyst injection compared mESCs. However, here we showed can be incorporated into normal development by forced expression E-cadherin transgene for 2 days culture. Using this...
Abstract Hemopoietic cells develop in a complex milieu that is made up of diverse components, including stromal cells. Wnt genes, which are known to regulate the fate variety tissues, expressed hemopoietic organs. However, their roles hemopoiesis not well characterized. In this study, we examined proteins using conditioned medium containing Wnt-3a. This dramatically reduced production B lineage and myeloid cells, except for macrophages long-term bone marrow cultures grown on although...
Nitration of tyrosine residues in proteins has been observed many inflammatory tissues arthritis, ulcerative colitis, septic shock and ischemia-reperfusion injury. Although several studies have carried out, it is still unclear what type protein nitrated whether nitration interferes with function. Peroxisome proliferator-activated receptor gamma (PPARgamma) a nuclear whose activation linked to physiological pathways including regulation insulin sensitivity control inflammation. PPARgamma...