A5007933005- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- Developmental Biology and Gene Regulation
- RNA Interference and Gene Delivery
- Cancer, Lipids, and Metabolism
- Prostate Cancer Treatment and Research
- Lipid metabolism and biosynthesis
- Biomedical Ethics and Regulation
- Evolution and Genetic Dynamics
- DNA Repair Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Evolutionary Game Theory and Cooperation
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Congenital heart defects research
- Birth, Development, and Health
- Mechanisms of cancer metastasis
- Angiogenesis and VEGF in Cancer
Kindai University
2018-2024
Universidad Católica San Antonio de Murcia
2017-2018
Salk Institute for Biological Studies
2012-2016
Tohoku University
2005-2013
Osaka Medical Center for Cancer and Cardiovascular Diseases
2003-2008
Japan Science and Technology Agency
2003-2008
Osaka University
2003-2004
Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, derivation stable ESCs domestic ungulates is important for genomic testing selection, genome engineering, modeling human diseases. Cattle one most that commonly used food bioreactors. To date, however, it remains a challenge to produce pluripotent bovine ESC lines. Employing culture system containing fibroblast growth factor 2 an inhibitor canonical...
Abstract Meiosis is a unique process that allows the generation of reproductive cells. It remains largely unknown how meiosis initiated in germ cells and why non-germline do not undergo meiosis. We previously demonstrated knockdown Max expression, gene encoding partner MYC family proteins, strongly activates expression cell-related genes ESCs. Here we find complete ablation ESCs results profound cytological changes reminiscent undergoing meiotic cell division. Furthermore, our analyses...
Abstract Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report disabling pancreatogenesis in pig embryos via zygotic co-delivery Cas9 mRNA dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent pluriopotent stem cells may serve as a platform xeno-generation tissues organs pigs.
The germ cell lineage segregates from the somatic lineages in early embryos. Germ determination mice is not regulated by maternally inherited germplasm, but initiated within embryo during gastrulation. However, mechanisms of specification remain unknown. We located precursors to primordial cells (PGCs) embryos, and show here that cell-cell interaction among these required for specification. found expression a calcium-dependent adhesion molecule, E-cadherin, restricted proximal region...
Vasculogenesis and hematopoiesis are thought to arise in hemangioblasts,the common progenitors of cells vessels blood. This scheme was challenged by kinetic analysis vascular endothelial hematopoietic early gastrulating mouse embryos. The OP-9 co-culture system with a combination cytokines permitted the detection progenitors, as well stroma-dependent progenitors. Endothelial were detected embryonic day (E) 5.50,after which time their numbers increased. Stroma-dependent at E6.75, point when...
Abstract Mouse primordial germ cells (PGCs) are initially identified as a cluster of alkaline phosphatase (AP)‐positive within the extraembryonic mesoderm near posterior part primitive streak at embryonic day (E) 7.25. Clonal analysis epiblast has revealed that putative precursors PGCs localized in proximal epiblast, and we demonstrated conditions required for PGC formation induced region epiblasts by ectoderm. Bone morphogenetic protein (BMP) 4 BMP8b, which belong to transforming growth...
Transcription elongation is stimulated by positive transcription factor b (P-TEFb), for which activity repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of snRNP growth control primordial germ cells (PGCs). The expression p15 INK4b , cyclin-dependent kinase inhibitor (CDKI) gene, PGCs selectively activated P-TEFb and its recruiting molecule, Brd4, when amount active increased due to reduction snRNP, consequently undergo arrest. These...
Primordial germ cells (PGCs) are the founder of germline. Via gametogenesis and fertilisation this lineage generates a new embryo in next generation. PGCs also cell origin multilineage teratocarcinomas. In vitro, mouse can give rise to embryonic (EG) – pluripotent stem that contribute primary chimaeras when introduced into pre-implantation embryos. Thus, course developmental cycle, during teratocarcinogenesis by vitro culture. However, there is no evidence differentiate directly somatic...
Serum-containing medium is widely used to support cell attachment, stable growth and serial passaging of various cancer lines. However, the presence cholesterols lipids in serum greatly hinders analysis effects cholesterol depletion on cells culture. In this study, we developed a defined serum-free culture condition accessible variety different types adherent cells. We tested factors that are considered essential for extracellular matrix plate coating, found cultured Dulbecco’s Modified...
To produce muscle fibers for cultured meat on a large scale, it is important to expand myoblasts in serum-reduced or serum-free medium avoid cost, ethical, and environmental issues. Myoblasts such as C2C12 cells differentiate quickly into myotubes lose their ability proliferate when the serum-rich replaced with medium. This study demonstrates that Methyl-β-cyclodextrin (MβCD), starch-derived agent depletes cholesterol, can inhibit further differentiation of at MyoD-positive stage by reducing...