A5031061515- Mitochondrial Function and Pathology
- HIV-related health complications and treatments
- Metabolism and Genetic Disorders
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Adipose Tissue and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Innovations in Medical Education
- Parkinson's Disease Mechanisms and Treatments
- Health and Medical Education
- Healthcare Systems and Technology
- Diet and metabolism studies
- Alcohol Consumption and Health Effects
- Inflammatory Myopathies and Dermatomyositis
- Birth, Development, and Health
- Genetic Neurodegenerative Diseases
- Meta-analysis and systematic reviews
- Neonatal Health and Biochemistry
- COVID-19 Clinical Research Studies
- HIV/AIDS Research and Interventions
- Bone and Joint Diseases
- Cytomegalovirus and herpesvirus research
- Genetics and Neurodevelopmental Disorders
- Systemic Lupus Erythematosus Research
- Infectious Encephalopathies and Encephalitis
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2014-2023
Centre for Biomedical Network Research on Rare Diseases
2014-2023
Universitat de Barcelona
2014-2023
Hospital Clínic de Barcelona
2011-2023
Instituto de Investigación de Enfermedades Raras
2008-2022
Instituto de Salud Carlos III
2018-2022
Hospital Sant Joan de Déu Barcelona
2010-2022
Centro de Investigación Biomédica en Red
2012-2021
Departament de Salut
2006-2020
Federación Española de Enfermedades Raras
2015
<b>Objective</b> To investigate the effect of an additional review based on reporting guidelines such as STROBE and CONSORT quality manuscripts. <b>Design</b> Masked randomised trial. <b>Population</b> Original research manuscripts submitted to <i>Medicina Clínica</i> journal from May 2008 April 2009 considered suitable for publication. <b>Intervention</b> Control group: conventional peer reviews alone. Intervention plus looking missing items guidelines. <b>Outcomes</b> Manuscript quality,...
Abstract: Carbon monoxide (CO) toxicity is the result of a combination tissue hypoxia and direct CO‐mediated damage at cellular level, since not all signs symptoms presented can be explained only by formation carboxyhaemoglobin. Mitochondria, specially electron transport chain, seem to target for CO subcellular level. However, effect in individual complexes human mitochondrial respiratory chain has been completely elucidated. We here studied vitro on mitochondria. obtained muscle from 10...
Background. Sepsis is associated with mitochondrial dysfunction and impaired oxygen consumption, which may condition clinical outcome independent of tissue oxygenation. However, role in sepsis severity remains unknown. We aimed to characterize function sepsis, establish its origin cellular consequences, determine correlation symptoms outcome. Methods. Different markers activity, nitrosative oxidative stress, apoptosis, inflammation were measured peripheral blood mononuclear cells (PBMCs)...
Different abnormalities in mitochondrial electron transport chain activity have been demonstrated muscle and other tissues of patients with idiopathic Parkinson's disease (PD). We studied eight Spanish PD to evaluate the functional mitochondria from this country. found lower complex I (nmol.min-1.mg-1) (245.8 +/- 42.8) than controls (331.6 60.1) (p = 0.004) IV (46.1 9) (144.1 42.3) 0.00001). Complex V was also decreased two II III activities were normal all them. Although these results...
The objective of the present study was to determine mitochondrial toxicity mechanisms linezolid-related hyperlactatemia. Five patients on a long-term schedule linezolid treatment were studied during acute phase hyperlactatemia and after clinical recovery lactate normalization following withdrawal. Mitochondrial studies performed with peripheral blood mononuclear cells consisted measurement mass, protein synthesis homeostasis (cytochrome c oxidase [COX] activity, COX-II subunit expression,...
Abstract Background The use of remdesivir has demonstrated a significant reduction in the time to recovery patients with COVID-19. However, impact on mortality is still controversial. Therefore, it necessary evaluate whether there specific subgroup whom an active antiviral therapy also reduces mortality. Methods Patients admitted for &gt;48 h our hospital SARS-CoV-2 confirmed or suspected infection from February 2020 2021 were retrospectively analysed. primary outcome study was at 30...
Occasionally, a temporal artery biopsy reveals small-vessel vasculitis (SVV) surrounding spared artery, the significance of which is unclear. We analyzed final diagnosis in series patients with this condition and tried to identify histopathologic features potential usefulness predicting ultimate diagnosis.We performed clinical review 28 whom SVV was first histologic finding that led vasculitis. For comparison purposes, we pattern small vessel involvement 30 biopsy-proven giant cell arteritis...
Wolfram syndrome is a progressive neurodegenerative disorder transmitted in an autosomal recessive mode. We report two families harboring multiple deletions of mitochondrial DNA. The reached percentages as high 85-90% affected tissues such the central nervous system one patient, while other from same patient and members family, deleted DNA genomes were only 1-10%. Recently, gene has been linked to markers on 4p16. In both linkage between disease locus 4p16 gave maximum multipoint lod score...
BackgroundAlthough peer review is widely considered to be the most credible way of selecting manuscripts and improving quality accepted papers in scientific journals, there little evidence support its use. Our aim was estimate effects on manuscript either adding a statistical reviewer or suggesting use checklists such as CONSORT STARD clinical reviewers both.Methodology Principal FindingsInterventions were defined 1) addition process, 2) reporting guidelines reviewers; with “no expert”...
Objectives The management of HIV infection has greatly improved during recent years essentially because the appearance new antiretroviral drugs. Highly active therapy (HAART) achieved important reductions viraemia and significant recoveries CD4+ cell counts in HIV-infected patients. Nonetheless, cases individuals experiencing lipodystrophy (LD) are being increasingly reported. purpose this work was to analyse whether presence mitochondrial abnormalities is a frequent feature LD, since we...
It is now widely acknowledged that a large number of human diseases originate from respiratory-chain dysfunctions. Because the molecular bases these are still poorly known, biochemical approach has to be used in screening procedures for diagnoses conditions. Assessment function samples faces several problems: (i) small size available samples, (ii) determination discriminating parameters, and (iii) interfering factors, such as age physical activity. The present study focuses on isolated...
A decrease in the mitochondrial (mt) DNA to nuclear ratio has gained acceptance as a marker of toxicity treated HIV-infected patients, but functional meaning this alteration is unclear.We assessed mtDNA content, content and function peripheral blood mononuclear cells (PBMCs) consecutive asymptomatic patients. Patients selected had been receiving first-line highly active antiretroviral therapy (HAART) regimen for at least 6 months, consisting zidovudine plus lamivudine or stavudine didanosine...
A limitation in the use of classic neuroleptic drugs is eventual appearance extrapyramidal symptoms. Some studies, mainly based on experimental situations, have related these symptoms with a defect mitochondrial electron transport chain (ETC), especially complex I (CI). One advantages "atypical" neuroleptics lower incidence movement disorders. We studied peripheral blood mononuclear cells from naive schizophrenic patients (n = 25) and under chronic treatment 1 "typical" (haloperidol, n 15)...
Objective: Antiretrovirals, especially thymidine-analogue nucleoside reverse transcriptase inhibitors (tNRTIs), may cause the mitochondrial damage in adipose tissue that has been associated with lipodystrophy development. HIV itself blood cell mitochondria. However, viral capacity to induce lesion is still a matter of doubt. We aimed assess whether untreated infection was abnormalities. Design: Single-site, cross-sectional, controlled observational and exploratory study without intervention....
The antibiotic linezolid is a ribosomal inhibitor with excellent efficacy. Although the administration period has been reduced to 28 days, side effects, usually of hematologic or neuropathic origin, are still reported due secondary inhibition mitochondrial protein synthesis. Susceptibility toxicity remains unknown. Therefore, objective this study was gain an understanding clinical heterogeneity in response identical exposures through exhaustive examination molecular basis tissue-dependent...
In utero exposure of fetuses to tobacco is associated with reduced birth weight. We hypothesized that this may be due the toxic effect carbon monoxide (CO) from tobacco, which has previously been described damage mitochondria in non-pregnant adult smokers. Maternal peripheral blood mononuclear cells (PBMCs), newborn cord (CBMCs) and placenta were collected 30 smoking pregnant women their newborns classified as moderate severe groups, compared a cohort 21 non-smoking controls. A biomarker for...
Sporadic inclusion body myositis (sIBM) is one of the most common myopathies in elderly people. Mitochondrial abnormalities at histological level are present these patients. We hypothesize that mitochondrial dysfunction may play a role disease aetiology. took following measurements muscle and peripheral blood mononuclear cells (PBMCs) from 30 sIBM patients 38 age- gender-paired controls: DNA (mtDNA) deletions, amount mtDNA mtRNA, protein synthesis, respiratory chain (MRC) complex I IV...
From 2005 to 2010, we conducted 2 randomized studies on a journal (Medicina Clínica), where took manuscripts received for publication and randomly assigned them either the standard editorial process or additional processes. Both were based use of methodological reviewers reporting guidelines (RG). Those interventions slightly improved items reported Manuscript Quality Assessment Instrument (MQAI), which assesses quality research report. However, masked evaluators able guess allocated group...