A5038915641- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Respiratory viral infections research
- SARS-CoV-2 detection and testing
- Influenza Virus Research Studies
- Eicosanoids and Hypertension Pharmacology
- Fire effects on ecosystems
- Long-Term Effects of COVID-19
- HVDC Systems and Fault Protection
- Venous Thromboembolism Diagnosis and Management
- Immune cells in cancer
- Pancreatic and Hepatic Oncology Research
- Respiratory Support and Mechanisms
- Dermatological and COVID-19 studies
- Radiomics and Machine Learning in Medical Imaging
- Glioma Diagnosis and Treatment
- COVID-19 and healthcare impacts
Erasmus MC
2019-2025
Erasmus University Rotterdam
2019-2023
Codarts Rotterdam
2023
University Medical Center Utrecht
2022
Icahn School of Medicine at Mount Sinai
2020
Halting transmission The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) glycoprotein binds to host cells and initiates membrane fusion cell infection. This stage in the virus life history is currently a target for drug inhibition. De Vries et al. designed highly stable lipoprotein inhibitors complementary conserved repeat C terminus of S that integrate into membranes inhibit conformational changes necessary fusion. authors tested performance lipoproteins as...
Abstract Effective clinical intervention strategies for coronavirus disease 2019 (COVID-19) are urgently needed. Although several trials have evaluated use of convalescent plasma containing virus-neutralizing antibodies, levels neutralizing antibodies usually not assessed and the effectiveness has been proven. We show that hamsters treated prophylactically with a 1:2560 titer human or 1:5260 monoclonal antibody were protected against weight loss, had significant reduction virus replication...
Infections with New World hantaviruses are associated high case fatality rates, and no specific vaccine or treatment options exist. Furthermore, the biology of hantaviral GnGc complex, its antigenicity, fusion machinery poorly understood. Protective monoclonal antibodies against have potential to be developed into therapeutics disease also great tools elucidate glycoprotein complex.
Morbidity and mortality from influenza are associated with high levels of systemic inflammation. Endothelial cells play a key role in inflammatory responses during severe A virus (IAV) infections, despite being rarely infected humans. How endothelial contribute to is unclear. Here, we developed transwell system which airway organoid–derived differentiated human lung epithelial were co-cultured primary microvascular (LMECs). We compared the susceptibility LMECs pandemic H1N1 recent seasonal...
SARS-CoV-2 attaches to angiotensin-converting enzyme 2 (ACE2) gain entry into cells after which the spike protein is cleaved by transmembrane serine protease (TMPRSS2) facilitate viral-host membrane fusion. ACE2 and TMPRSS2 expression profiles have been analyzed at genomic, transcriptomic, single-cell RNAseq levels. However, transcriptomic data actual validation convey conflicting information regarding distribution of biologically relevant receptor in whole tissues. To describe organ-level...
Orthohantaviruses are emerging zoonotic viruses that can infect humans via the respiratory tract. There is an unmet need for in vivo model to study infection of different orthohantaviruses physiologically relevant tissue and assess efficacy novel pan-orthohantavirus countermeasures. Here, we describe use a human lung xenograft mouse permissiveness orthohantavirus species its utility preclinical testing therapeutics. Following xenografted tissues, distinct differentially replicated...
Abstract Containment of the COVID-19 pandemic requires reducing viral transmission. SARS-CoV-2 infection is initiated by membrane fusion between and host cell membranes, mediated spike protein. We have designed a dimeric lipopeptide inhibitor that blocks this critical first step for emerging coronaviruses document it completely prevents in ferrets. Daily intranasal administration to ferrets prevented direct-contact transmission during 24-hour co-housing with infected animals, under stringent...
Seoul orthohantavirus (SEOV) is a rat-borne zoonotic virus that transmitted via inhalation of aerosolized infectious excreta, and can cause hemorrhagic fever with renal syndrome (HFRS) in humans worldwide. In rats, SEOV predominantly exists as persistent infection the absence overt clinical signs. Lack disease rats attributed to downregulation pro-inflammatory upregulation regulatory host responses. As lung microvascular endothelial cells (LMECs) represent primary target both human...
ABSTRACT Effective clinical intervention strategies for COVID-19 are urgently needed. Although several trials have evaluated the use of convalescent plasma containing virus-neutralizing antibodies, effectiveness has not been proven. We show that hamsters treated with a high dose human or monoclonal antibody were protected against weight loss showing reduced pneumonia and pulmonary virus replication compared to control animals. However, ten-fold lower showed no protective effect. Thus,...
Thrombosis is a frequent and severe complication in patients with coronavirus disease 2019 (COVID-19) admitted to the intensive care unit (ICU). Lupus anticoagulant (LA) strong acquired risk factor for thrombosis various diseases frequently observed COVID-19. Whether LA associated COVID-19 currently unclear.To investigate if critically ill COVID-19.The presence of other antiphospholipid antibodies was assessed ICU. determined dilute Russell's viper venom time (dRVVT) LA-sensitive activated...
The Omicron BA.1 (B.1.1.529) SARS-CoV-2 variant is characterized by a high number of mutations in the viral genome, associated with immune escape and increased spread. It remains unclear whether milder COVID-19 disease progression observed after infection humans due to reduced pathogenicity virus or pre-existing immunity from vaccination previous infection. Here, we inoculated hamsters evaluate kinetics shedding, compared Delta (B.1.617.2) animals re-challenged 614G infected showed clinical...
Puumala orthohantavirus (PUUV) is endemic in Europe and can cause hemorrhagic fever with renal syndrome (nephropathia epidemica). Disease features include fever, thrombocytopenia, acute kidney injury (AKI). This retrospective cohort study of forty PUUV patients aims to characterize associations serum immunological, hemostatic or markers disease severity. While interleukin-18 (IL-18) was significantly increased severely thrombocytopenic (<100 × 109 platelets/L) compared higher platelet...
Abstract Orthohantaviruses are emerging zoonotic viruses that can infect humans via the respiratory tract. There is a clear unmet need for an in vivo model to study orthohantavirus infection physiologically relevant tissue and determine broad reactivity of novel countermeasures. Here, we describe use human lung xenograft mouse permissiveness different species assess its utility preclinical testing pan-orthohantavirus therapeutics. Following xenografted tissues, distinct differentially...
We studied PUUV infection in high-throughput 3D endothelial vessels-on-chip, that more closely resemble the vivo situation than static 2D cultures. In these vessels, increased monocyte adhesion, but not vascular permeability.
Abstract SARS-CoV-2 attaches to angiotensin-converting enzyme 2 (ACE2) gain entry into cells after which the spike protein is cleaved by transmembrane serine protease (TMPRRS2) facilitate viral-host membrane fusion. ACE2 and TMPRRS2 expression profiles have been analyzed at genomic, transcriptomic, single-cell RNAseq level, however, biologically relevant receptor organization in whole tissues still poorly understood. To describe organ-level architecture of expression, related ability mediate...
Abstract The Omicron BA.1 (B.1.1.529) SARS-CoV-2 variant is characterized by a high number of mutations in the viral genome, associated with immune-escape and increased spread. It remains unclear whether milder COVID-19 disease progression observed after infection humans due to reduced pathogenicity virus or pre-existing immunity from vaccination previous infection. Here, we inoculated hamsters evaluate kinetics shedding, compared Delta (B.1.617.2) animals re-challenged 614G infected showed...
Abstract Morbidity and mortality from influenza are associated with high levels of systemic inflammation. Endothelial cells have been shown to play a key role in this inflammatory response during severe A virus (IAV) infections, despite the fact that these rarely infected humans. However, how endothelial contribute responses is unclear. To investigate this, we developed transwell-system which airway organoid-derived differentiated human lung epithelial at apical side were co-cultured primary...