Xanthine oxidase (XO) is an important enzyme catalyzing the hydroxylation of hypoxanthine to xanthine and uric acid which excreted by kidneys. Excessive production and/or inadequate excretion results in hyperuricemia. This paper presents a detailed review methods isolation, determination activity, effect plant extracts their constituents on it. Determining content activities XO can be used for diagnostic purposes. Testing inhibition detection potentially effective compounds or that treat...

Purpose: The aim of this work was to determine the content total phenols, flavonoids, anthocyanins, as well antioxidant and antimicrobial activities hawthorn (Crataegus oxyacantha L.) alcohol, hydroalcohol aqueous extracts.Methods: flavonoids anthocyanins extracts were determined using spectrophotometric methods. Antioxidant assay based on measurement DPPH absorbance at 517 nm caused by reaction with test sample. Antimicrobial activity evaluated measuring zone inhibition against selected...

Blackthorn (Prunus spinosa L.) is commonly used in food industry and phytotherapy. The contents of phenols, flavonoids, anthocyanins antioxidative activity extracts blackthorn fruit were determined using spectrophotometric methods. content total phenol compounds varies from 15.33 to 20.94 mg GAE g-1 fresh fruit. flavonoids very low, ranges 0.419 1.31 QE fruits. Anthocyanins lies between 0.112 cyanidin 3-glucoside/g sample ethanol extract 0.265 3-glucoside methanol-water 50/50 (v/v) extract....

Purpose: To develop and validate an accurate, sensitive reproducible high performance liquid chromatographic (HPLC) method for the quantitation of vitamin C in pharmaceutical samples.Method: The drug standard were eluted from Superspher RP-18 (250 mm x 4.6 mm, 10ìm particle size) at 20 0C. mobile phase was prepared by carefully adding acetic acid (500 ml) to 1.5g 1-hexanesulfonic sodium salt mixing well (pH 2.6). flow rate 0.7 mL min-1. UV detector, set 280 nm, used monitor effluent.Results:...

The simplest approach to enhance alginate hydrogel characteristics and functional properties is replace the calcium in process of gelation with other metallic ions which are essential for living systems. Gelling using modern encapsulation techniques can provide new delivery systems required properties. Hence, this study Cu-alginate hydrogels form microbeads were produced by electrostatic extrusion gelling solutions Cu(II) concentrations range 13.5-270 mM comprehensively characterized vitro....

The heavy metals (Fe, Cu, Zn and Mn) contents of selected plant species, grown in Southeast region Serbia, that are traditionally used alternative medicine were determined. Among the considered metals, iron content was highest one varied from 137.53 up to 423.32 mg/kg, while Mn remarkably lower, ranged 8.91 62.20 mg/kg. In addition, an analysis plants extracts showed a significant transfer during extraction procedure; therefore, corresponding coefficients reached values 88.8%. Those...

Peach (Prunus persica L.) is a fruit of high nutritional and economic value. Carbohydrates, dietary fibers, minerals organic acids are among the major constituents peach fruit, which contribute to quality both fresh fruits juice. Polyphenolic compounds found in may play an important role physiological functions related human health. Different polyphenolics have varied biological activities including antioxidant activity. In this study characteristics between peel pulp different cultivars...

An accurate, sensitive and reproducible high performance liquid chromatographic (HPLC) method for the quantification of doxycycline hyclate in pharmaceutical samples has been developed validated. The drug standard were eluted from a Lichrosorb RP-8 (250 mm x 4.6 mm, 10 particle size) at 20?C with mobile phase consisting methanol, acetonitrile 0.010 M aqueous solution oxalic acid (2:3:5, v/v/v). flow rate was 1.25 ml min-1. A UV detector set 350 nm used to monitor effluent. Each analysis...

Abstract The methanol extracts of four Parmeliaceae lichens (Hypogymnia physodes, Evernia prunastri, Flavoparmelia caperata and Parmelia sulcata) were screened for antioxidant properties total phenol content. H. physodes extract was the most effective at reducing iron(III) scavenging 1,1-diphenyl-2-picrylhydrazyl radicals, while P. sulcata in molybdenum(VI) an acidic medium. E. prunastri contained more Folin-Ciocalteu reagent reactive substances than F. extracts. Significant activity DPPH...

The influence of the solvent concentration, pH, temperature, sampler solid ratio and extraction time on extractability total phenolic compounds from barley was investigated. most effective (30% aqueous ethanol solution with 5% HCl v/v) used for modeling solid-liquid polyphenolic at 30 mL/g (the volume per gram raw material). Total in extracts were determined spectrophotometrically using Folin-Ciocalteu method 760 nm. content depends conditions applied. mechanism is confirmed to occur...

Introduction.Among different fruit species, aronia have attracted a great attention because of its wide range protective effects with potential benefits for human health.Furthermore, the consumption products (juice, tea, berries) is becoming part healthy lifestyle.Materials and Methods.The multi-element characterization has been carried out using inductively coupled plasma atomic emission spectrometry (ICP-AES).Results Discussion.Among major elements, most abundant are K, Ca P, followed by...

Total phenolic and flavonoid contents of different extracts of Papaver rhoeas L. were examined. High total compounds (9.73 -19.91 mg GAE/g fresh petals) flavonoids (7.904 - 11.45 QE/g determined. Red pigment present in the flowers of P. originates from anthocyanins, which may act as natural antioxidants. Anthocyanins content investigated is very unified ranged 4.72 to 5.193 cyanidin-3-O-glukoside/g petals. The antioxidant activity was tested using spectrophotometric method by means ability...

The aim of this study is to evaluate the content phenolics: total phenols (TP), flavonoids (TF), anthocyanins (TA), as well antioxidant capacity (TAC) in three sour cherry cultivars (Prunus cerasus L.) introduced southeast Serbia climate conditions. Among researched cherries, ?Oblacinska? cultivar contained highest amounts all groups phenolics, followed by ?Cigancica? > ?Marela?. A significant difference were observed phenolic among different and growing seasons (p<0.05), compounds...

Introduction . Mulberry is the most medicinally important plant of genus Morus The mulberry fruit used for many medical purposes. species origin and adaptation, description, foliage, flowers fruit, location, soil, pests diseases are summarized. Composition studies from different countries show that rich in phenolic compounds, macro-elements (K, Ca, Mg, Na) micro-elements (Fe, Zn, Ni). Phenolic compounds found all parts plant. Uses can be making jam, jelly, pulp, drinks, sauce cake. fruits...

The absorption and fluorescence emission spectra of cyanidin 3-O-?-glucopyranoside (Cy3Glc) at pH 5.5 in aqueous solution have been studied. most effective excitations were the UV maxima 220 230 nm higher wavelengths 270 280 nm. Cyanidin exhibits 310 visible range 615 For Cy3Glc excitation was 300 has with maximum 380 does not show range. If compare it can be seen that intensity for is significantly than Cy3Glc. These results reveals impact 3-glucosidic substitution C-3 aglycone (to form...

Fruits and vegetables constitute the cheapest source of essential trace elements for majority people living in developing countries. The Cu, Fe Zn contents twenty selected wine samples produced South-East region Serbia were determined by flame atomic absorption spectrometry. Cu concentrations varied from 0.07 to 0.57 ppm wines, fluctuated 2.93 36.2 ppm, while levels range 0.21 0.67 ppm. established showed that wines this part world could serve as good dietary sources metals, values within...

The content of phenolics: total phenols (TP), flavonoids (TF), anthocyanins (TA) and hydroxicinnamic acid as well the antioxidant capacity (TAC) in nine commercial red fruit juices (sour cherry, black currant, grape) produced Serbia were evaluated. compounds was measured by spectrophotometric methods, TAC determined using DPPH assays, individual hydroxycinnamic acids HPLC-DAD methods. Among examined juices, currant contained highest amounts all groups phenolics exhibited strong capacity....

The content of total phenols, flavonoids and antioxidant activities different plant extracts Callendula Officinalis L., used in Serbian traditional medicine were determined. phenol was determined by using UV-Vis measurements at 765 nm the tested obtained standard extraction Calendula officinalis L. extracts, examined DPPH method showed a high activity that correlated significantly with phenols flavonoids. All exhibited strong scavenging against radicals (more than 96,8%). contents metals...