A5045888605- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Intraocular Surgery and Lenses
- Corneal surgery and disorders
- BRCA gene mutations in cancer
- Ocular Infections and Treatments
- Retinal Imaging and Analysis
- CRISPR and Genetic Engineering
- Ocular Oncology and Treatments
- Blood groups and transfusion
- Retinal and Optic Conditions
- Blood donation and transfusion practices
- Genomics and Chromatin Dynamics
- Corneal Surgery and Treatments
- Retinal Development and Disorders
- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Chronic Kidney Disease and Diabetes
- Ophthalmology and Eye Disorders
- Cancer and Skin Lesions
- Ureteral procedures and complications
- Employee Performance and Management
- Retinopathy of Prematurity Studies
- Ocular Surface and Contact Lens
- Cutaneous lymphoproliferative disorders research
College of Medicine & Sagore Dutta Hospital
2025
Midnapore Medical College and Hospital
2018-2024
Hindu Rao Hospital
2023-2024
All India Institute of Medical Sciences Rishikesh
2023
Ministry of Health and Population
2023
Veer Kunwar Singh University
2023
Career Point University
2023
Medical College and Hospital, Kolkata
2005-2021
Bir Hospital
2019
Institute of Medical Sciences
2019
Inherited mutations in BRCA1 confer susceptibility to breast and ovarian neoplasms. However, the function of role noninherited cancer remain unknown. Characterization alternately spliced forms may identify functional regions; thus, we constructed expression vectors a splice variant lacking exon 11, designated delta 672-4095. Immunofluorescence studies indicate nuclear localization but cytoplasmic Two putative signals (designated NLS1 NLS2) were identified 11; immunofluorescence that only is...
AbstractA drastic reduction in BRCA1 gene expression is a characteristic feature of aggressive sporadic breast carcinoma. However, the mechanisms underlying downregulation cancer are not well understood. Here we report that both vitro and vivo HMGA1b protein binds to inhibits activity human mouse promoters. Consistently, murine embryonic stem (ES) cells with Hmga1 deleted display higher Brca1 mRNA levels than do wild-type ES cells. Stable transfection MCF-7 cDNA results decrease lack...
Cytokines such as interleukin-1β (Il-1) are produced in the brain during development and inflammatory processes that result from lesions or disease. One function of Il-1 appears to be stimulation astrocytes proliferate produce a variety cytokines trophic factors, including nerve growth factor. The mechanisms by which exerts its actions on remain poorly defined. We present evidence this cytokine elicits activation NFκB transcription factor mediates effects mRNA expression. Elucidation...
Objective: The objective of this study was to assess the role inflammation in pathogenesis diabetic retinopathy (DR) using neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR), and mean platelet volume (MPV) compare these parameter levels between DR non-DR (NDR) subjects. Materials Methods: A cross-sectional conducted among 60 patients with a definite diagnosis type 2 diabetes mellitus attending ophthalmology outpatient department tertiary care hospital. We obtained blood...
Introduction: Glaucoma represents a group of disease defined by consistent remodeling the connective tissue elements optic nerve head and with loss neural retinal fiber layer associated eventual development distinctive patterns visual function.1 OCT is used commonly to evaluate thinning peripapillary (RNFL) objectively quantitatively, which hallmark for diagnosis glaucoma.2 As OCT, normal database commercially not available Nepalese eyes. These measurements will provide reference comparison...
Since the majority of high-grade breast cancers express reduced levels BRCA1 mRNA, we investigated factors regulating transcription. Factors with specific affinity for previously identified positive regulatory region (PRR) in promoter were purified from whole-cell extracts. Identified proteins included replication protein A and a series related sense strand PRR. subset activated promoter. Identification these families represents an important step comprehension mechanisms responsible cancer...
A bstract Background: The purpose of the present study was to determine modes presentation retinoblastoma and association between proptosis with orbital extension histopathologically detectable optic nerve infiltration by tumour. Methods: Clinical records histopathological reports patients were studied retrospectively. work undertaken at a tertiary care hospital in eastern Nepal during period from September 1995 August 2002. Results: total 43 studied. Among them unilateral involvement found...
Inherited mutations in the BRCA1gene, presumably leading to loss of function, confer susceptibility breast and ovarian neoplasms are thought be responsible for approximately 2.5–5% all cancers. It has been suggested that alternative mechanisms, such as disruption transcription, may also involved suppression BRCA1 gene expression/function Therefore, we initiated studies on transcriptional promoter. Utilizing systematic promoter deletions transient transfection assays, a 36-base pair region...
The corneal endothelium plays an important role in maintaining the dehydrated state and transparency of cornea. Some degree endothelial cell loss invariably occurs all types cataract surgery but amount varies with surgical technique.To evaluate density central cells before after small incision (SICS) posterior chamber intraocular lens (PC IOL) implantation.A total 100 eyes patients undergoing SICS PC IOL were included study. Endothelial was measured non-contact specular microscope...
To study the epidemiology of chronic dacryocystitis and success rate external dacryocystorhinostomy (DCR) in Nepal.This is a retrospective descriptive interventional case series. A total 662 records patients with due to nasolacrimal duct obstruction, who were treated by means DCR without bicanalicular silastic tube intubation, studied.Mean for age standard deviation confidence intervals calculated. The significance difference was calculated using unpaired t-test.Of patients, 67.6% female....
Forecasting consumption of blood products can reduce their order frequency by 60% and inventory level 40%. This also prevents shortage balancing demand supply. The study aimed to establish a "Simple Average with Mean Annual Increment" (SAMAI) method time series forecasting compare its results those ARIMA, ratio trend, simple average forecast products. Monthly data component from January 2017 December 2022 (data set I) was used for creating model. To avoid the effect COVID19 pandemic instead...
Abstract Background We investigated the molecular basis of primary open-angle glaucoma (POAG) using Opticin ( OPTC ) as a candidate gene on its expression in trabecular meshwork cells involved disease pathogenesis. Two hundred POAG patients and 100 controls were enrolled this study. The coding sequence was amplified by PCR from genomic DNA patients, followed SSCP, DHPLC sequencing. Subsequent bioinformatic analysis, site-directed mutagenesis, quantitative RT-PCR western blot experiments...
Background: Diabetic nephropathy is one of the dreaded complications diabetes leading to chronic kidney disease and end stage renal failure globally. Microalbuminuria most sensitive marker early recognition diabetic nephropathy. This study was carried out find prevalence potential risk factors microalbuminuria which among patients in Nepal. Methods: A cross-sectional conducted on a random sample 227 T2DM private clinics Bir hospital Kathmandu. Data were collected using standard questionnaire...
An integrated approach involving physical mapping, identification of transcribed sequences, and computational analysis genomic sequence was used to generate a detailed transcription map the 1.0-Mb region containing breast cancer susceptibility locusBRCA2on chromosome 13q12–q13. This is included in genetic interval bounded byD13S1444andD13S310.Retrieved sequences from exon amplification or hybrid selection procedures were grouped into intervals subsequently units by clone overlap. Overlap...
Transfusion Transmissible Infections (TTIs) such as human immune-deficiency virus (HIV-I/II), hepatitis B (HBV), Hepatitis C (HCV), Malaria parasite (MP) and syphilis can spread through contaminated blood or products. The present study was designed to analyze the prevalence of TTIs their association with group, among donors Delhi. Blood group determined by hem-agglutination using Gel card. HIV, HBV, HCV test performed ELISA, RPR MP rapid card method. A total 345(2.038%) were positive for...
The purpose of the present paper was to report spectrum primary malignant tumours eye and adnexa at BP Koirala Institute Health Sciences, Nepal, from 1995 2000.A retrospective study medical records with histopathological confirmation done for years 1995-2000. A total 116 consecutive Department Pathology Sciences were retrieved. All those patients ophthalmic malignancies included non-malignant cases excluded.There 80 which 39 (48.8%) male 41 (51.2%) female. Four had bilateral involvement....
Diabetic nephropathy is one of the dreaded complications diabetes leading to chronic kidney disease and end stage renal failure globally. Microalbuminuria most sensitive marker early recognition diabetic nephropathy. This study was carried out find prevalence potential risk factors microalbuminuria which among patients in Nepal.A cross-sectional conducted on a random sample 227 T2DM private clinics Bir hospital Kathmandu. Data were collected using standard questionnaire format collect...
Aim: To compare the statistical supremacy of patients undergoing cataract surgeries at a tertiary eye care center in pre-COVID versus post-COVID period. Methods: A complete audit attending our ophthalmology department and surgery was compared between August 2019 to March 2020 (Group A) 2021 B). All pertinent ophthalmological parameters were recorded detail for comparison. Results: Average daily OPD attendance Groups B 146.7 111.2 ( P = 0.004) respectively. However, average monthly done these...