A5069926062- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion Transport and Channel Regulation
- Protein Kinase Regulation and GTPase Signaling
- Neurogenesis and neuroplasticity mechanisms
- Memory and Neural Mechanisms
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Nerve injury and regeneration
- Adenosine and Purinergic Signaling
- Sleep and Wakefulness Research
- Neural dynamics and brain function
- Stress Responses and Cortisol
- Pain Mechanisms and Treatments
- Photochromic and Fluorescence Chemistry
- Neuroscience of respiration and sleep
- Anesthesia and Neurotoxicity Research
- Nicotinic Acetylcholine Receptors Study
- Pharmacological Receptor Mechanisms and Effects
- Pain Management and Placebo Effect
- Nitric Oxide and Endothelin Effects
University of Missouri–Kansas City
2016-2025
Capital Medical University
2015-2017
Capital University
2017
Saint Luke's Hospital
2004-2011
Division of Undergraduate Education
2005-2006
Tongji Hospital
2005
Czech Academy of Sciences, Institute of Physiology
2002
East Carolina University
1994-1998
Howard University
1998
Group I metabotropic glutamate receptors (mGluRs) increase cellular levels of inositol-1,4,5-triphosphate (IP3) and thereby trigger intracellular Ca2+ release. Also, group mGluRs are organized with members Homer scaffold proteins into multiprotein complexes involved in postreceptor signaling. In this study, we investigated the relative importance IP3/Ca2+ signaling novel mGluR-mediated activation extracellular signal-regulated protein kinases 1 2 (ERK1/2) cultured rat striatal neurons. We...
The specification and organization of glutamatergic synaptic transmission require the coordinated interaction among glutamate receptors their adaptor proteins closely assembled in postsynaptic density (PSD). Here we investigated between NMDA metabotropic receptor 5 (mGluR5) integral regulation extracellular signal-regulated protein kinase (ERK) gene expression cultured rat striatal neurons. We found that coapplication mGluR5 agonist ( S )-3,5-dihydroxyphenylglycine synergistically increased...
Abstract The role of N‐methyl‐D‐aspartate (NMDA) excitatory amino acid receptors in D‐amphetamine (AMPH)‐induced behavioral changes and increased expression the nuclear transcription factors, c‐ fos zif / 268 , preprodynorphin (PPD) mRNA various regions rat forebrain was investigated with quantitative situ hybridization histochemistry. Three hours after a single injection AMPH (5 mg/kg, i.p.), but not dorsal striatum (caudate nucleus) cerebral cortex (sensorimotor cortex), PPD striatum,...
Anesthetics may interact with ionotropic glutamate receptors to produce some of their biologic actions. Cellular studies reveal that the receptors, N-methyl-D-aspartate (NMDARs), can be phosphorylated on NR1 subunits at C-terminal serine residues, which is a major mechanism for regulation NMDAR functions. It currently unknown whether anesthetics have any modulatory effects subunit phosphorylation.The possible effect general anesthetic propofol phosphorylation 897 (pNR1S897) and 896...
The regulation of protein phosphorylation requires coordinated interaction between kinases and phosphatases (PPs). Recent evidence has shown that the Galphaq-protein-coupled metabotropic glutamate receptor (mGluR) 5 up-regulates MAPK/ERK1/2. However, signaling mechanisms linking mGluR5 to ERK are poorly understood. In this study, roles a major serine/threonine PP, PP2A, in event were evaluated cultured neurons. We found PP1/2A inhibitors okadaic acid calyculin A mimicked effect agonists...
Chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein-1, plays a critical role in leukocyte recruitment and activation. In the present study, we identify an additional for CCL2 that of neuroprotection against HIV-1 transactivator protein (Tat) toxicity rat primary midbrain neurons. Furthermore, report involvement transient receptor potential canonical (TRPC) channels CCL2-mediated neuroprotection. TRPC are Ca 2+ -permeable, nonselective cation with variety...
In the present study, changes of amino acids release in spinal cord after application angiotensin II (ANG II) rostral ventrolateral medulla (RVLM) and distribution ANG receptors on neurons RVLM were investigated. A microdialysis experiment showed that microinjection into significantly ( P < 0.01) increased aspartate glutamate intermediolateral column cord. Immunofluorescence technique combined with confocal microscopy demonstrated most glutamatergic GABAergic both Wistar spontaneously...
Our previous work has demonstrated a dose-dependent induction of striatal preprodynorphin (PPD) in response to single injection the psychostimulants amphetamine (AMPH) or methamphetamine (METH). In present study, dose-response effects acute administration these stimulants on preproenkephalin (PPE) mRNA expression rat striatum were investigated with quantitative situ hybridization histochemistry 3 h after injection. Acute AMPH METH at equimolar doses (3.75, 7.5, 15, and 30 μmol/kg)...
Abstract Extracellular signals may regulate mitogen‐activated protein kinase (MAPK) cascades through a receptor‐mediated mechanism. As signaling superhighway to the nucleus, active Ras‐MAPK phosphorylate transcription factors and facilitate gene expression. In cultured rat striatal neurons, present work systemically examined linkage between glutamate receptors extracellular signal‐regulated 1/2 (ERK1/2) subclass of MAPK. We found that induced rapid transient phosphorylation ERK1/2. Similar...
The glutamate receptor adaptor protein Homer is concentrated in the postsynaptic density of excitatory synapses and critical for normal operation synaptic transmission. In this study, we investigated responsiveness family proteins to dopamine stimulation with psychostimulant cocaine rat striatal neurons both vivo vitro. We found that a single dose specifically induced rapid transient increase levels Homer1a, but not Homer1b/c Homer2a/b, isoforms striatum. This selective Homer1a induction was...
Post-translational covalent modifications of glutamate receptors remain a hot topic. Early studies have established that this family receptors, including almost all ionotropic and metabotropic receptor subtypes, undergoes active phosphorylation at serine, threonine, or tyrosine residues in their intracellular domains. Recent evidence identifies several subtypes to be direct substrates for palmitoylation cysteine residues. Other such as ubiquitination sumoylation lysine also occur certain...