A5070988495- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- Solar and Space Plasma Dynamics
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Advanced Biosensing Techniques and Applications
- SARS-CoV-2 detection and testing
- Microfluidic and Capillary Electrophoresis Applications
- Membrane Separation Technologies
- Nanopore and Nanochannel Transport Studies
- Single-cell and spatial transcriptomics
- Enhanced Oil Recovery Techniques
- Respiratory viral infections research
- Analytical Chemistry and Chromatography
- Advancements in Transdermal Drug Delivery
- Molecular Biology Techniques and Applications
- Quantum Dots Synthesis And Properties
- Bacterial Infections and Vaccines
- 3D Printing in Biomedical Research
- Lipid Membrane Structure and Behavior
- Calcium Carbonate Crystallization and Inhibition
- Polymer Surface Interaction Studies
- Minerals Flotation and Separation Techniques
Liaoning Technical University
2025
East China University of Science and Technology
2024
Environmental Molecular Sciences Laboratory
2017-2023
The University of Texas at El Paso
2014-2022
Pacific Northwest National Laboratory
2017-2021
Division of Chemistry
2019
Richland College
2018
Battelle
2018
John Wiley & Sons (United States)
2018
Ocean University of China
2011-2013
Bacterial meningitis is a serious health concern worldwide. Given that can be fatal and many cases occurred in high-poverty areas, simple, low-cost, highly sensitive method great need for immediate early diagnosis of meningitis. Herein, we report versatile cost-effective polydimethylsiloxane (PDMS)/paper hybrid microfluidic device integrated with loop-mediated isothermal amplification (LAMP) the rapid, sensitive, instrument-free detection main meningitis-causing bacteria, Neisseria...
Effective extension of mass spectrometry-based proteomics to single cells remains challenging. Herein we combined microfluidic nanodroplet technology with tandem tag (TMT) isobaric labeling significantly improve analysis throughput and proteome coverage for mammalian cells. Isobaric facilitated multiplex cell-sized protein quantities a depth ∼1 600 proteins median CV 10.9% correlation coefficient 0.98. To demonstrate in-depth high cell analysis, the platform was applied measure expression in...
Based on the nanoparticle-mediated photothermal effect, a new biomolecular quantitation method using common thermometer as signal reader was developed.
Current mass spectrometry (MS)-based proteomics approaches are ineffective for mapping protein expression in tissue sections with high spatial resolution because of the limited overall sensitivity conventional workflows. Here we report an integrated and automated method to advance spatially resolved by seamlessly coupling laser capture microdissection (LCM) a recently developed nanoliter-scale sample preparation system termed nanoPOTS (Nanodroplet Processing One pot Trace Samples). The...
Enzyme linked immunosorbent assay (ELISA) is one of the most widely used laboratory disease diagnosis methods. However, performing ELISA in low-resource settings limited by long incubation time, large volumes precious reagents, and well-equipped laboratories. Herein, we developed a simple, miniaturized paper/PMMA (poly(methyl methacrylate)) hybrid microfluidic microplate for low-cost, high throughput, point-of-care (POC) infectious diagnosis. The novel use porous paper flow-through...
Abstract Large numbers of cells are generally required for quantitative global proteome profiling due to surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic impossible rare cell populations (e.g., circulating tumor (CTCs)). Here we report a surfactant-assisted one-pot preparation coupled mass spectrometry (MS) method termed SOP-MS label-free single-cell proteomics. capitalizes...
A polymer/paper hybrid microfluidic SpinChip integrated with DNA probe-functionalized GO nanosensors was developed for simple and effective multiplex quantitative LAMP (μ-mqLAMP) detection.
Pertussis is a highly contagious respiratory disease caused by the bacterium Bordetella pertussis (B. pertussis). The infection difficult to diagnose especially in underserved or resource-limited areas. We developed low-cost and instrument-free diagnostic method for rapid accurate detection of B. on point-of-care (POC) testing device.We paper/polymer hybrid microfluidic biochip integrated with loop-mediated isothermal amplification (LAMP) pertussis. This approach was validated 100...
To address insolubility issues in detection of organic chemicals, we developed an innovative interfacial nanosensing strategy microfluidic droplets for high-sensitivity 17β-estradiol and other low-solubility molecules.
Two-dimensional reversed-phase capillary liquid chromatography (2D RPLC) separations have enabled comprehensive proteome profiling of biological systems. However, milligram sample quantities proteins are typically required due to significant losses during offline fractionation. Such a large requirement generally precludes the application samples in nanogram low-microgram range. To achieve in-depth proteomic analysis such small-sized samples, we developed nanoFAC (nanoflow Fractionation and...
This study presents a novel methodology for the separation of quartz and feldspar under neutral weakly alkaline conditions. Ethyl-bis(dodecyl dimethyl ammonium bromide) (Gemini) is introduced as new collector in flotation process, combined with sodium oleate (NaOL) to enhance efficiency. The investigation focuses on adsorption mechanism these collectors mineral surfaces. While individual collectors, Gemini NaOL, were ineffective differentiating between feldspar, their combination resulted...
Miniaturized multidimensional peptide separations can greatly increase the coverage in proteome profiling for trace samples.
Due to sensitivity limitations, global proteome measurements generally require large amounts of biological starting material, which masks heterogeneity within the samples and differential protein expression among constituent cell types. Methods for spatially resolved proteomics are being developed resolve distinct types highly heterogeneous tissues, but have primarily been applied mammalian systems. Here we evaluate performance cell-type-specific analysis tomato fruit pericarp tissues by a...
Bacterial meningitis, an infection of the membranes (meninges) and cerebrospinal fluid (CSF) surrounding brain spinal cord, is one major causes death disability worldwide. Higher case-fatality rates short survival times have been reported in developing countries. Hence, a quick, straightforward, low-cost approach great demand for diagnosis meningitis. In this research, microfluidic fully paper-based analytical device (μFPAD) integrated with loop-mediated isothermal amplification (LAMP)...
A low-cost b̲a̲ttery-powered s̲pectrophotometric s̲ystem (BASS) was developed for high-sensitivity point-of-care analysis in low-resource settings on a microfluidic chip without relying external power supplies.