A5062121701- Mycorrhizal Fungi and Plant Interactions
- Legume Nitrogen Fixing Symbiosis
- Advanced Proteomics Techniques and Applications
- Soil Carbon and Nitrogen Dynamics
- Mass Spectrometry Techniques and Applications
- Microbial Community Ecology and Physiology
- Environmental DNA in Biodiversity Studies
- Peatlands and Wetlands Ecology
- Forest Ecology and Biodiversity Studies
- Diverse Educational Innovations Studies
- Biosensors and Analytical Detection
- Ecology and Vegetation Dynamics Studies
- Lichen and fungal ecology
- Plant Pathogens and Fungal Diseases
- Plant and fungal interactions
- Gender and Technology in Education
- Glycosylation and Glycoproteins Research
- Agronomic Practices and Intercropping Systems
- Plant Pathogens and Resistance
- Seedling growth and survival studies
- Pesticide and Herbicide Environmental Studies
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- RNA modifications and cancer
Pacific Northwest National Laboratory
2020-2024
Environmental Molecular Sciences Laboratory
2024
University of Tennessee at Knoxville
2023
William Paterson University
2006-2022
Washington State University
2021
University of West Florida
2005-2006
Environmental Protection Agency
2006
Ithaca College
2006
Cornell University
2006
Oregon State University
1991-2003
Abstract Background The Internal Transcribed Spacer (ITS) regions of fungal ribosomal DNA (rDNA) are highly variable sequences great importance in distinguishing species by PCR analysis. Previously published primers available for amplifying these from environmental samples provide varying degrees success at discriminating against plant while maintaining a broad range compatibility. Typically, it has been necessary to use multiple primer sets accommodate the fungi under study, potentially...
Abstract Large numbers of cells are generally required for quantitative global proteome profiling due to surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic impossible rare cell populations (e.g., circulating tumor (CTCs)). Here we report a surfactant-assisted one-pot preparation coupled mass spectrometry (MS) method termed SOP-MS label-free single-cell proteomics. capitalizes...
To better understand temporal variability in soil denitrification, denitrifying enzyme activity (DEA) and denitrifier populations (as determined by most-probable-number [MPN] counts) were measured field laboratory experiments. Measurements of DEA MPN provided highly contradictory indications dynamics. In incubations, under conditions favoring active the synthesis new enzymes actual amount denitrification closely related. other experiments, however, both counts poor indicators...
Abstract Intestinal stem cells are non-quiescent, dividing epithelial that rapidly differentiate into progenitor of the absorptive and secretory cell lineages. The kinetics this process is rapid such epithelium replaced weekly. To determine how transcriptome proteome keep pace with differentiation, we developed a new sorting method to purify mouse colon cells. Here show alternative mRNA splicing polyadenylation dominate changes in as progenitors. In contrast, progenitors mature types, levels...
Soil bacterial predators that use the biomass of hosts for growth (multiplication), energy, or replication have potential to reduce populations in wide variety terrestrial ecosystems which they are found. Bacterial predators, including bacteria-feeding nematodes, protists, bacteria ( Bdellovibrio and like organisms, Lysobacter, myxobacteria), bacteriophages responsible turnover soils lead many ecosystem services. The demonstrated breadth specificity host ranges these make them interesting...
Recent advances in sample preparation enable label-free mass spectrometry (MS)-based proteome profiling of small numbers mammalian cells. However, specific devices are often required to downscale processing volume from the standard 50–200 μL sub-μL for effective nanoproteomics, which greatly impedes implementation current nanoproteomics methods by proteomics research community. Herein, we report a facile one-pot method termed SOPs-MS (surfactant-assisted at coupled with MS) convenient robust...
A number of methods are available for those researchers considering the addition molecular analyses ectomycorrhizal (EcM) fungi to their research projects and weighing various approaches they might take. Analyzing natural EcM fungal communities has traditionally been a highly skilled, time‐consuming process relying heavily on exacting morphological characterization root tips. Increasingly powerful analyzing make this area much wider range researchers. Ecologists can gain from body work...
We examined the relationship between relative abundance of ectomycorrhizas in soil cores determined using morphotype tip counts and terminal restriction fragment length polymorphism (TRFLP) analysis. Root tips were harvested from a total 120 collected six family plots loblolly pine ( Pinus taeda L.) genetics plantation. Tips each core morphotyped based on physical characteristics, identified through TRFLP sequence analysis, then pooled to reconstruct ectomycorrhizal community within that...
Actinorhizal nodules do not usually evolve H 2 due to the action of an uptake hydrogenase. We have found that several Frankia symbioses evolved large amounts gas when returned air following exposure 10 kPa C HT during acetylene reduction assay. Increased evolution in persisted for days intact root systems Alnus incana (L.) Moench (inoculated with UGL 011101) were treated C.H 1 h. Full recovery hydrogenase activity required 4 8 days. Studies crude homogenates same plants showed (measured...
We compared three methods for estimating fungal species diversity in soil samples. A rapid screening method based on gross colony morphological features and color reference standards was with traditional taxonomic PCR-RFLP estimation of ecological indices microfungal community composition. Normalized counts morphotypes dichloran rose bengal medium were used to estimate richness (S) evenness ( J) calculate Shannon's (H) Simpson's (SI) dominance indices. Isolates obtained by dilution plating...
Protein analysis of small numbers human cells is primarily achieved by targeted proteomics with antibody-based immunoassays, whereas they have inherent limitations (e.g., low multiplex and unavailability antibodies for new proteins). Mass spectrometry (MS)-based has emerged as an alternative in terms being antibody-free, high multiplex, specificity, quantitation accuracy. Recent advances MS instrumentation make MS-based possible multiplexed quantification highly abundant proteins single...
Abstract A nested polymerase chain reaction (PCR) protocol using unique primers was developed to detect and quantify Myxococcus species from environmental samples. The amplified most of when 10 pg DNA representing 1000 cells present, although over half were with as little 0.1 (10 cells). did not amplify other myxobacterial species, members the δ‐proteobacteria or unrelated organisms tested at significantly higher concentrations DNA. also used in quantitative PCRs, which accurately estimated...
Protein analysis of small numbers human cells is primarily achieved by targeted proteomics with antibody-based immunoassays, which have inherent limitations (e.g., low multiplex and unavailability antibodies for new proteins). Mass spectrometry (MS)-based has emerged as an alternative because it antibody-free, high multiplex, specificity quantitation accuracy. Recent advances in MS instrumentation make MS-based possible multiplexed quantification highly abundant proteins single cells....