A5100742368- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Lung Cancer Diagnosis and Treatment
- Chemical Reactions and Isotopes
- Tracheal and airway disorders
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Medical Imaging and Pathology Studies
- Pleural and Pulmonary Diseases
- Remote Sensing and Land Use
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Land Use and Ecosystem Services
- Cardiac Ischemia and Reperfusion
- Microtubule and mitosis dynamics
- Cancer Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Trauma Management and Diagnosis
- 3D Printing in Biomedical Research
Northwestern University
2017-2025
Peking University People's Hospital
2020-2025
Peking University
2020-2025
Shanxi Medical University
2013-2025
Sichuan University
2025
Xi'an Jiaotong University
2010-2025
First Affiliated Hospital of Xi'an Jiaotong University
2025
Midwestern University
2018-2025
Anhui Medical University
2021-2025
Fujian Agriculture and Forestry University
2025
To examine the role of breast cancer stem cells (BCSCs) in metastasis, we generated human-in-mouse orthotopic models using patient tumor specimens, labeled with optical reporter fusion genes. These recapitulate human features not captured previous models, including spontaneous metastasis particular, and provide a useful platform for studies initiation progression. With noninvasive imaging approaches, as few 10 stably BCSCs could be tracked vivo, enabling early growth metastasis. advances...
Abstract Circulating tumor cells (CTC) seed cancer metastases; however, the underlying cellular and molecular mechanisms remain unclear. CTC clusters were less frequently detected but more metastatic than single CTCs of patients with triple-negative breast representative patient-derived xenograft models. Using intravital multiphoton microscopic imaging, we found that clustered in migration circulation resulted from aggregation individual rather collective cohesive shedding. Aggregated...
Exosomes play a pivotal role in mediating intercellular communications and package delivery. They have recently been discovered to serve as diagnostic biomarkers well possible drug delivery vehicle based on their nanometer size range capability transfer biological materials recipient cells. Their unique biocompatibility, high stability, preferred tumor homing, adjustable targeting efficiency can make exosomes an attractive potentially effective tool of cancer therapy. While possess...
Abstract Circulating tumor cell (CTC) clusters mediate metastasis at a higher efficiency and are associated with lower overall survival in breast cancer compared to single cells. Combining single-cell RNA sequencing protein analyses, here we report the profiles of primary cells lung metastases triple-negative (TNBC). ICAM1 expression increases by 200-fold three TNBC patient-derived xenografts (PDXs). Depletion abrogates colonization inhibiting homotypic cell-tumor cluster formation. Machine...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the pandemic of induced disease 2019 (COVID-19) with evolving variants concern. It remains urgent to identify novel approaches against broad strains SARS-CoV-2, which infect host cells via entry receptor angiotensin-converting enzyme (ACE2). Herein, we report an increase in circulating extracellular vesicles (EVs) that express ACE2 (evACE2) plasma COVID-19 patients, levels are associated pathogenesis....
Abstract Traumatic brain injuries (TBI) and stroke are major causes of morbidity mortality in both developing developed countries. The complex heterogeneous pathophysiology TBI cerebral ischemia‐reperfusion injury (CIRI), addition to the blood‐brain barrier (BBB) resistance, is a advancement diagnostics therapeutics. Clinical data showed that severity positively correlated with number neutrophils peripheral blood sites. Furthermore, neutrophil extracellular traps (NETs) released by correlate...
Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due their stability, accessibility representation of molecules from source cells. However, this potential has been stymied by lack approaches for molecular profiling individual exosomes, which have diameter 30-150 nm. Here we report rapid analysis evaluate heterogeneous surface protein expression single circulating blood. Our studies show differential CD47 blood-derived that is...
A luminescent lanthanide MOF-based thin film was fabricated by electrodeposition in an anhydride system and this can be used as a highly selective sensor for CO<sub>3</sub><sup>2−</sup> aqueous solution.
Purpose: Effective targeting of cancer stem cells is necessary and important for eradicating reducing metastasis-related mortality. Understanding stemness-related signaling pathways at the molecular level will help control stop metastasis in clinic.Experimental Design: By analyzing miRNA profiles functions development, we aimed to identify regulators breast tumor stemness human xenograft models vivo examined their effects on self-renewal invasion vitro To discover direct targets essential...
// Abhineet Uppal 1 , Sean C. Wightman Stephen Mallon 2, 3 Go Oshima P. Pitroda Qingbei Zhang 4 Xiaona Huang Thomas E. Darga Lei 5 Jorge Andrade Huiping Liu 6 Mark K. Ferguson 1, Geoffrey L. Greene 3, 7 Mitchell Posner Samuel Hellman Nikolai N. Khodarev * Ralph R. Weichselbaum Department of Surgery, The University Chicago, IL 60637, USA 2 Radiation and Cellular Oncology, Ludwig Center for Metastasis Research, Pathology, Committee on Cancer Biology, Research Informatics, Case Comprehensive...
Highlights•Stimulation of CD95 induces cancer stemness through induction type I IFNs•Cancer stem cells express a STAT1-regulated gene signature•Radiation-resistant SCC and CD95-stimulated breast an IFN signature•Type IFNs stimulate cellsSummaryStimulation CD95/Fas drives maintains (CSCs). We now report that this involves activation signal transducer activator transcription 1 (STAT1) genes process is inhibited by active caspases. STAT1 enriched in CSCs cell lines, patient-derived human...
Abstract Large numbers of cells are generally required for quantitative global proteome profiling due to surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic impossible rare cell populations (e.g., circulating tumor (CTCs)). Here we report a surfactant-assisted one-pot preparation coupled mass spectrometry (MS) method termed SOP-MS label-free single-cell proteomics. capitalizes...
Triple-negative breast cancer (TNBC) is one of the most aggressive and metastatic subtypes lacking targeted therapy. Our recent work demonstrated that circulating tumor cell (CTC) clusters polyclonal metastasis TNBC are driven by aggregation CD44+ stem cells (CSC) associated with an unfavorable prognosis, such as low overall survival. However, there no existing therapeutic can specifically block CTC or CSC cluster formation. Methods: Using patient-derived xenograft (PDX) models, we...
Despite advances in breast cancer therapy, effective targeting of stem cells (CSCs) remains a challenge. CSCs, which have self-renewal, tumorigenic and metastatic properties, are often quiescent located hypoxic regions tumors [1], making them resistant to conventional chemo- radiotherapies [2]. These characteristics allow CSCs survive, leading relapse metastasis. Studying under conditions similar their niche is essential for evaluating therapies that target these cells. We show can be...