A5089754631- Chemical Reactions and Isotopes
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Autoimmune Bullous Skin Diseases
- Medical Imaging and Pathology Studies
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Urticaria and Related Conditions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Drug-Induced Adverse Reactions
- Lung Cancer Diagnosis and Treatment
- Biosensors and Analytical Detection
- Antibiotic Resistance in Bacteria
- Nail Diseases and Treatments
- Cutaneous lymphoproliferative disorders research
- Cancer-related molecular mechanisms research
- 3D Printing in Biomedical Research
- Bacterial Identification and Susceptibility Testing
- Circular RNAs in diseases
- Mechanisms of cancer metastasis
- Streptococcal Infections and Treatments
- Autoimmune and Inflammatory Disorders
- Cancer Diagnosis and Treatment
- Medicine and Dermatology Studies History
- RNA modifications and cancer
Temple University
2020-2024
Thomas Jefferson University
2023-2024
Baylor College of Medicine
2017-2023
Thomas Jefferson University Hospital
2023
Jefferson University Hospitals
2023
Temple University Hospital
2021-2023
Chinese Center For Disease Control and Prevention
2017-2022
American Academy of Dermatology
2022
Beijing Center for Disease Prevention and Control
2019
Case Western Reserve University
2014-2018
Abstract Circulating tumor cells (CTC) seed cancer metastases; however, the underlying cellular and molecular mechanisms remain unclear. CTC clusters were less frequently detected but more metastatic than single CTCs of patients with triple-negative breast representative patient-derived xenograft models. Using intravital multiphoton microscopic imaging, we found that clustered in migration circulation resulted from aggregation individual rather collective cohesive shedding. Aggregated...
Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due their stability, accessibility representation of molecules from source cells. However, this potential has been stymied by lack approaches for molecular profiling individual exosomes, which have diameter 30-150 nm. Here we report rapid analysis evaluate heterogeneous surface protein expression single circulating blood. Our studies show differential CD47 blood-derived that is...
Purpose: Effective targeting of cancer stem cells is necessary and important for eradicating reducing metastasis-related mortality. Understanding stemness-related signaling pathways at the molecular level will help control stop metastasis in clinic.Experimental Design: By analyzing miRNA profiles functions development, we aimed to identify regulators breast tumor stemness human xenograft models vivo examined their effects on self-renewal invasion vitro To discover direct targets essential...
Abstract This study established a constant-temperature fluorescence quantitative detection method, combining loop-mediated isothermal amplification (LAMP) with molecular beacons. The advantages of LAMP are its convenience and efficiency, as it does not require thermocycler results easily visualized by the naked eye. However, major disadvantage current techniques is use indirect evaluation methods (e.g., electrophoresis, SYBR Green I dye, precipitation, hydroxynaphthol blue turbidimetric...
Klebsiella pneumoniae is a wide-spread nosocomial pathogen. A rapid and sensitive molecular method for the detection of K. in clinical samples needed to guide therapeutic treatment. In this study, we first described loop-mediated isothermal amplification (LAMP) capsular polysaccharide synthesis regulating gene rcsA from pneumoniaein by using two methods including real-time turbidity monitoring fluorescence assess reaction. Then dissemination strains was investigated ICU patients three top...
A novel New Delhi metallo-β-lactamase (NDM) variant, NDM-14, was identified in clinical isolate Acinetobacter lwoffii JN49-1, which recovered from an intensive care unit patient at a local hospital China. differs other existing enzymes by amino acid substitution position 130 (Asp130Gly), possesses enzymatic activity toward carbapenems that is greater than of NDM-1. Kinetic data indicate NDM-14 has higher affinity for imipenem and meropenem.
Pseudomonas aeruginosa is a major opportunistic pathogen in hospital-acquired infections and exhibits increasing antibiotic resistance. A rapid sensitive molecular method for its detection clinical samples needed to guide therapeutic treatment control P. outbreaks. In this study, we established polymerase spiral reaction (PSR) of by targeting the toxA gene, which regulates exotoxin synthesis. Real-time turbidity monitoring chromogenic visualization using hydroxynaphthol blue were used assess...
Candida albicans is the most common human yeast pathogen which causes mucosal infections and invasive fungal diseases. Early detection of this needed to guide preventative therapeutic treatment. The aim study was establish a polymerase spiral reaction (PSR) assay that rapidly accurately detects C. assess clinical applicability PSR-based diagnostic testing. Internal transcribed spacer 2 (ITS2), region between 5.8S 28S ribosomal DNA, used as target sequence. Four primers were designed for...
Abstract Patient-derived human-in-mouse xenograft models of breast cancer (PDX models) that exhibit spontaneous lung metastases offer a potentially powerful model metastasis. In this study, we evaluated the malignant character micrometastases emerge in such after orthotopic implantation human tumor cells into mouse mammary fat pad. Interestingly, relative to parental primary tumors, metastasis (met)–derived tumors exhibited slower growth rate and reduced metastatic potential with more...
The emergence of the plasmid-encoded colistin-resistance gene mcr-1 in Enterobacteriaceae represents a new threat to treatment infection clinical setting. A sensitive and rapid molecular method for detection isolates is needed control spread this gene. In study, we established loop-mediated isothermal amplification (LAMP) assay This was applied cultured bacteria spiked human stools. Real-time monitoring turbidity chromogenic visualization were used assess reaction results. specificity...
Abstract Two novel New Delhi metallo-β-lactamase-1 (NDM-1)-positive plasmids containing a complete composite transposon, Tn 125 , from two respective Acinetobacter towneri isolates were characterized. Plasmid pNDM-GJ01 (30,293 bp) isolated A . G165 did not show homology to any known plasmid structure, except for the transposon bla NDM-1 repB gene and XRE-type transcriptional regulators found in pNDM-GJ01. pNDM-GJ02 (62,011 G295 showed highest pBJAB0715 (41% coverage, 99% nucleotide...
Mycobacterium tuberculosis is an age-old bacterium that difficult to eliminate. A simple and rapid diagnostic method of great importance prevent the spread M. tuberculosis. Here, we developed a low-cost nucleic acid detection technique, named GenePop, which enabled storage transport reagent at ambient temperatures, without need for professional operations or expensive instrumentation. Using vitrification method, vitrified heat-unstable components onto cap reaction tube, placed heat-stable...
Citrobacter freundii, a Gram-negative bacterium, causes many opportunistic infections. Bacteriophage phiCFP-1 was isolated and characterized by its ability to lyse the multidrug-resistant clinical C. freundii strain P10159. Transmission electron microscopy showed that phage has an icosahedral head short tail, making it Podoviridae family member. In single-step growth experiment, exhibited eclipse period of 20 min burst size 100 particles per cell. Its genome assembled as circular molecule...