A5084135451- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Pituitary Gland Disorders and Treatments
- Glioma Diagnosis and Treatment
- Cancer, Stress, Anesthesia, and Immune Response
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Hedgehog Signaling Pathway Studies
- Cancer therapeutics and mechanisms
- Endoplasmic Reticulum Stress and Disease
- Protein Structure and Dynamics
- Adrenal Hormones and Disorders
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Business Strategies and Management Research
- Heat shock proteins research
- Cancer Genomics and Diagnostics
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Colorectal and Anal Carcinomas
- Bacterial biofilms and quorum sensing
St. Luke's University Health Network
2023-2024
Ariel University
2021-2023
Columbia University
2019-2022
The University of Texas MD Anderson Cancer Center
2022
Drexel University
2018-2021
University of Michigan
2006-2020
A. Alfred Taubman Health Care Center
2020
Public Health Foundation of India
2018
Indian Council of Medical Research
2018
National Institute of Cancer Prevention and Research
2018
Abstract The epithelial components of the mammary gland are thought to arise from stem cells with a capacity for self-renewal and multilineage differentiation. Furthermore, these and/or their immediate progeny may be targets transformation. We have used both in vitro cultivation xenograft mouse model examine role hedgehog signaling Bmi-1 regulating normal malignant human cells. show that PTCH1, Gli1, Gli2 highly expressed stem/progenitor cultured as mammospheres genes down-regulated when...
Abstract We have used in vitro and mouse xenograft models to examine the interaction between breast cancer stem cells (CSC) bone marrow–derived mesenchymal (MSC). show that both of these cell populations are organized a cellular hierarchy which primitive aldehyde dehydrogenase expressing regulate CSCs through cytokine loops involving IL6 CXCL7. In NOD/SCID mice, labeled MSCs introduced into tibia traffic sites growing tumor xenografts where they accelerated growth by increasing CSC...
Abstract Circulating tumor cells (CTC) seed cancer metastases; however, the underlying cellular and molecular mechanisms remain unclear. CTC clusters were less frequently detected but more metastatic than single CTCs of patients with triple-negative breast representative patient-derived xenograft models. Using intravital multiphoton microscopic imaging, we found that clustered in migration circulation resulted from aggregation individual rather collective cohesive shedding. Aggregated...
MicroRNAs (miRNAs) play important roles in normal cellular differentiation and oncogenesis. microRNA93 (mir-93), a member of the mir106b-25 cluster, located intron 13 MCM7 gene, although frequently overexpressed human malignancies may also function as tumor suppressor gene. Using series breast cancer cell lines representing different stages mouse xenograft models, we demonstrate that mir-93 modulates fate stem cells (BCSCs) by regulating their proliferation states. In "claudinlow" SUM159...
Pancreatic ductal adenocarcinoma (PDAC) remains one of the leading causes cancer-related deaths in world. For patients with PDAC who are not eligible for surgery, radiation therapy improves local disease control, yet safely delivering therapeutic doses challenging due to off-target toxicities surrounding normal tissues. NBTXR3, a novel radioenhancer composed functionalized hafnium oxide crystalline nanoparticles, has recently shown clinical activity soft tissue sarcoma, hepatocellular...
Abstract Strategies that interfere with the binding of receptor programmed cell death protein-1 (PD-1) to ligand-1 (PD-L1) have shown marked efficacy against many advanced cancers, including those are negative for PD-L1. Precisely why patients PD-L1 tumors respond PD-1/PD-L1 checkpoint inhibition remains unclear. Here, we show platelet-derived regulates growth and interference platelet cancer cells promotes T cell-induced cytotoxicity. These results suggest successful outcomes based...
Aberrant activation of the receptor tyrosine kinase-mediated RAS signaling cascade is primary driver embryonal rhabdomyosarcoma (ERMS), a pediatric cancer characterized by block in myogenic differentiation. To investigate cellular function activated regulating growth and differentiation ERMS cells, we genetically ablated oncogenes with high-efficiency genome-editing technology. Knockout NRAS CRISPR-inducible xenograft models resulted near-complete tumor regression through combination cell...
Staphylococcus aureus is a major human pathogen of the skin. The global burden diabetes high, with S. being complication diabetic wound infections. We investigated how environment influences skin infection and observed an increased susceptibility to in mouse models both type I II diabetes. A dual gene expression approach was taken investigate transcriptional alterations host bacterium after infection. While analysis response revealed only minor changes between infected control mice, we that...
Resistance to chemotherapy is a leading cause of treatment failure. Drug resistance mechanisms involve mutations in specific proteins or changes their expression levels. It commonly understood that happen randomly prior and are selected during the treatment. However, selection drug-resistant mutants culture could be achieved by multiple drug exposures cloned genetically identical cells thus cannot result from pre-existent mutations. Accordingly, adaptation must generation de novo upon Here...
Acute adrenal insufficiency in the trauma patient is underrecognized and impact poorly understood. Our hypothesis was that identification treatment of acute reduces mortality patients. Institutional Review Board approval for retrospective review a prospective database from Level 1 center 2002 to 2004 obtained. The study population included patients receiving cosyntropin stimulation test (250 microg) and/or random cortisol level based on our practice management guideline an intensive care...
Signet cell carcinomas of the colon are well documented in adult population, but this cancer incidence is very low adolescent population. A 17 year old male child presented with one month progressive abdominal pain. CT scan abdomen showed significant abnormality involving ascending characterized as marked mural thickening. Biopsy results indicated signet ring carcinoma. carcinoma presumed to be caused by genetic mutations just like other colorectal cancers. Treatment for same cancer. Surgery...
Human fibroblasts can switch between lamellipodia-dependent and -independent migration mechanisms on two-dimensional surfaces in three-dimensional (3D) matrices. RhoA GTPase activity governs the from low-pressure lamellipodia to high-pressure lobopodia response physical structure of 3D matrix. Inhibiting actomyosin contractility these cells reduces intracellular pressure reverts lamellipodial protrusions via an unknown mechanism. To test hypothesis that high physically prevents formation, we...
The major heat shock protein Hsp70 forms a complex with scaffold Bag3 that links it to components of signaling pathways. Via these interactions, the Hsp70-Bag3 module functions as proteotoxicity sensor controls cell signaling. Here, search for pathways regulated by complex, we utilized JG-98, an allosteric inhibitor blocks its interaction Bag3. RNAseq followed pathway analysis indicated several including UPR were activated JG-98. Surprisingly, only eIF2α-associated branch was activated,...
Affinity diagramming is an effective and efficient method for forming nuanced interpretations of wide-ranging, unstructured qualitative data; however, this does not scale well to large data sets. We propose a novel affinity system, called Qualitative Diagrammer (QuAD) that leverages computer-generated suggestions using deep learning address the scalability process. QuAD features automatic grouping jump-start process provides throughout reduce sifting through notes. In paper, we present...
Cyanobacteria from habitats within Gujarat have been poorly studied with regard to their diversity. In the present investigation eight morphologically distinct cyanobacterial isolates were obtained and characterized fresh water marine habitats. Identification was performed based on morphological features 16S rDNA sequences analysis. A phylogenetic tree sequence of prepared. Phylogenetic analysis clustered into two groups thus highlighting importance both genetic methods in studying
Finding synergistic drug combinations is an important area of cancer research. Here, we sought to rationally design with inhibitor BTK kinase, ibrutinib, which used for the treatment several types leukemia. We (a) a pooled shRNA screen identify genes that protect cells from drug, (b) identified protective pathways via bioinformatics analysis these gene sets, and (c) drugs inhibit pathways. Based on this analysis, established inhibitors proteasome mTORC1 could synergize ibrutinib both in...
Abstract There is increasing evidence that the growth and metastasis of many tumors, including breast cancer, are driven by a cellular population displaying stem cell properties. Like their normal counterparts, these cancer cells may be regulated MicroRNAs (miRNAs). We have previously demonstrated lines contain subpopulations with properties can isolated virtue expression Aldehyde dehydrogenase (ALDH) as assessed Aldefluor assay. compared miRNA in Aldefluor-positive Aldefluor-negative...
Abstract Disclosure: O.A. Aluko: None. S. Patel: A. Graul: M. Deshmukh: Introduction.Sex cord stromal cell tumors are rare of the ovary that usually occur inthe first twotothreedecades life. Specifically, steroid account for 0.1% all ovariantumors.Testosterone productionis typical, with hirsutism and virilizationbeing most commonsymptoms. Amarkedly elevated testosterone level >200ng/dl is animportant diagnostic threshold discriminatingandrogen- secreting from non-neoplastic lesions.We...
Abstract Disclosure: S. Patel: None. O.A. Aluko: E. Thwe: T. Gallagher: M. Deshmukh: Introduction: Immune checkpoint inhibitors have shown significant improvement in treatment of various cancers. Thyroiditis and hypophysitis are well-known reported adverse effects immune inhibitors. However, the co-existence thyroiditis patients treated with is rare. We hereby report a case patient secondary adrenal insufficiency likely to who was atezolizumab for hepatocellular carcinoma. Case Report: A 65...
Abstract Disclosure: O.A. Aluko: None. S. Patel: S.V. Silparshetty: T. Gallagher: Introduction: Ectopic ACTH mediated Cushing’s syndrome is an uncommon but treatable condition that poses time constrained diagnostic and therapeutic challenge due to severe morbidity as illustrated by our case below. Clinical case:A 65-year-old male was admitted with profound generalized weakness. He became restricted wheelchair from being able hike a trail at Yosemite national park 8 weeks prior. Recent...
COTTON, BRYAN A; GUILLAMONDEGUI, OSCAR D; CARPENTER, ROBERT O; MORRIS, JOHN PATEL, SHIVANI H; FLEMING, SLOAN Author Information