Histone methyltransferases (SDGs) and demethylases (JMJs) are well-established regulators of transcriptional responses in plants under adverse conditions. This study characterized SDG JMJ enzymes the cowpea (Vigna unguiculata) genome analyzed their expression patterns various stress conditions, including root dehydration mechanical injury followed by CABMV or CPSMV inoculation. A total 47 VuSDG genes were identified classified into seven distinct classes: I, II, III, IV, V, VI, VII....

The advancement of global warming and climate change requires strategic actions in understanding seeking interactions between plant species microorganisms that are more tolerant to water deficit. This research assessed the morpho-agronomic, physiological, gene expression responses two Passiflora cincinnata accessions (tolerant sensitive) deficit, focusing on their relationship with mycorrhization. A randomized design accessions, field capacities, four AMF inoculation treatments was used...

In the scope of present work, four SuperSAGE libraries have been generated, using bulked root tissues from drought-tolerant accessions as compared with sensitive genotypes, aiming to generate a panel differentially expressed stress-responsive genes. Both groups were submitted 24 hours water deficit stress. The produced 8,787,315 tags (26 bp) that, after exclusion singlets, allowed identification 205,975 unitags. Most relevant BlastN matches comprised 567,420 tags, regarding 75,404 unitags...

Abstract Background Despite the importance of osmoprotectants, no previous in silico evaluation high throughput data is available for higher plants. The present approach aimed at identification and annotation osmoprotectant-related sequences applied to short transcripts from a soybean HT-SuperSAGE (High Throughput Super Serial Analysis Gene Expression; 26-bp tags) database, also its comparison with other transcriptomic genomic different sources. Methods A curated set osmoprotectants related...

Drought is a significant constraint to yield increase in soybean. The early perception of water deprivation critical for recruitment genes that promote plant tolerance. DeepSuperSAGE libraries, including one control and bulk six stress times imposed (from 25 150 min root dehydration) drought-tolerant sensitive soybean accessions, allowed identify new molecular targets drought survey uncovered 120,770 unique transcripts expressed by the contrasting accessions. Of these, 57,610 aligned with...

One of the most challenging aspects RT-qPCR data analysis is identification reliable reference genes. Ideally, they should be neither induced nor repressed under different experimental conditions. To date, few genes have been adequately studied for sugarcane (Saccharum spp.) using statistical approaches. In this work, six candidate ( αTUB, GAPDH, H1, SAMDC, UBQ, and 25S rRNA) were tested gene expression normalization root tissues from drought-tolerant -sensitive accessions after continuous...

A partir de folhas, caules e raízes plantas pinha graviola coletadas em Pernambuco foram obtidos 110 90 isolados fúngicos endofíticos, respectivamente. Vinte nove selecionados avaliados quanto à produção enzimas extracelulares, através do método qualitativo placas com meios sólidos específicos, capacidade estimular o crescimento mudas pinha. Esses identificados como pertencentes aos gêneros Acremonium (10,34%), Aspergillus (3,45%), Chaetomium Colletotrichum Cylindrocladium (13,8%), Fusarium...

The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of four subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct isoforms expressed HT-SuperSAGE libraries from roots drought-tolerant -sensitive genotypes, respectively. At least 10 different potential isoform targets their respective unitags considered to be promising future studies especially development...

Drought is the most damaging among major abiotic stresses. Transcriptomic studies allow a global overview of expressed genes, providing basis for molecular markers development. Here, HT-SuperSAGE technique allowed evaluation four drought-tolerant cultivars and four-sensitive cultivars, after 24h irrigation suppression. We identified 9831 induced unitags from roots tolerant with different regulations by -sensitive applied stress. These proposal 15 whose profiles were validated RT-qPCR,...

Summary Pluripotency-associated transcription factors (PATFs) modulate gene expression during early mammalian embryogenesis. Despite a strong understanding of PATFs mouse embryogenesis, limited progress has been made in ruminants. This work aimed to describe the temporal eight both sheep and cattle preimplantation development. Transcript availability was evaluated by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) eggs, cleavage-stage embryos, morulae, blastocysts....

Abstract The extent to which mammalian cells share similar transcriptomes remains unclear. Notwithstanding, such cross-species gene expression inquiries have been scarce for defined cell types and most lack the dissection of regulatory landscapes. Therefore, work was aimed determine C-MYC relative across fibroblasts ( Ovis aries Bos taurus ) via RT-qPCR comprehensively explore its landscape by in silico tools. prediction transcription factor binding sites 2.5 kb upstream sequence revealed...

This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients.Parasite blood was assessed by qPCR at five time points, up 12 months post-diagnosis. Sixteen patients were followed up.A significant reduction observed after treatment (P < 0.0001). One patient had an increased 3 post-treatment and relapsed clinically month six.We have described use...

A salinidade é um fator comum de estresse abiótico que afeta a produção agrícola onde ela existe. Uma das estratégias para promover reincorporação áreas salinizadas e o aumento da produtividade consiste no desenvolvimento na seleção genótipos tolerantes, permitirá identificação parentais serem utilizados em cruzamentos. Esta pesquisa teve como objetivo identificar diploides bananeira tolerantes à futuros trabalhos melhoramento genético, visando obtenção cultivares adaptados solos salinos....

The rationale for gathering information from plants procuring nitrogen through symbiotic interactions controlled by a common genetic program sustainable biofuel production is the high energy demanding application of synthetic fertilizers. We curated sequence publicly available plant sugarcane, performed an analysis SYM pathway known to control symbiosis in other plants, and provide results, sequences literature links as online database.Sugarcane informations were downloaded nucEST database,...