A5022426223- Biotin and Related Studies
- S100 Proteins and Annexins
- Click Chemistry and Applications
- Cellular transport and secretion
- Medicinal Plants and Neuroprotection
- Pharmacological Receptor Mechanisms and Effects
- Neuropeptides and Animal Physiology
- Myasthenia Gravis and Thymoma
- Pharmacological Effects and Toxicity Studies
- Pain Mechanisms and Treatments
- Autoimmune Neurological Disorders and Treatments
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetic Neurodegenerative Diseases
Institute for Neurodegenerative Disorders
2021-2023
Emory University
2021-2023
University of Arizona
2015
Abstract Proteomic profiling of brain cell types using isolation-based strategies pose limitations in resolving cellular phenotypes representative their native state. We describe a mouse line for type-specific expression biotin ligase TurboID, vivo biotinylation proteins. Using adenoviral and transgenic approaches to label neurons, we show robust protein neuronal soma axons throughout the brain, allowing quantitation over 2000 neuron-derived proteins spanning synaptic proteins, transporters,...
•Cytosolic TurboID biotinylates >50% of the proteome in microglia and neuronal cells.•TurboID-NES has minimal impacts on cellular proteomic composition function.•About 1340 proteins labeled by differentiate from neurons vitro.•TurboID profiling captures microglial activation lipopolysaccharide. Different brain cell types play distinct roles development disease. Molecular characterization cell-specific mechanisms using type–specific approaches at protein (proteomic) level can provide...
ABSTRACT Isolation and proteomic profiling of brain cell types, particularly neurons, pose several technical challenges which limit our ability to resolve distinct cellular phenotypes in neurological diseases. Therefore, we generated a novel mouse line that enables type-specific expression biotin ligase, TurboID, via Cre-lox strategy for vivo proximity-dependent biotinylation proteins. Using adenoviral-based transgenic approaches, show striking protein neuronal bodies axons throughout the...
1.0 ABSTRACT Different brain cell types play distinct roles in development and disease. Molecular characterization of cell-specific mechanisms using type-specific approaches at the protein (proteomic) level, can provide biological therapeutic insights. To overcome barriers conventional isolation-based methods for proteomics, vivo proteomic labeling with proximity dependent biotinylation cytosolic proteins biotin ligase TurboID, coupled mass spectrometry (MS) labeled proteins, has emerged as...
Despite advancements in systems-level analyses to Alzheimer's disease, global proteomic profiling of brain homogenate cannot resolve molecular changes occurring distinct cell types. Proximity labeling proteins by the biotin-ligase TurboID, coupled with mass spectrometry (MS) biotinylated proteins, can cell-type specific proteomes from without type enrichment. Before in-vivo applications, it is important identify preferentially labeled TurboID mammalian cells, and directly compare whole...
Abstract Background Despite decades of research into the cellular and molecular contributions brain cell types to Alzheimer’s disease (AD) pathogenesis, there are no glial‐specific disease‐modifying therapies. To address this, our group has developed innovative cell‐specific in vivo biotinylation proteins (CIBOP) approach quantify astrocyte‐specific proteomic transcriptomic AD under homeostatic neuroinflammatory conditions. Method In CIBOP approach, TurboID, a biotin ligase, is selectively...
Biologically relevant insights into cellular disease mechanisms of neurons and glia can be obtained by complimentary molecular profiling the transcriptome proteome these cells. While mutually exclusive pipelines are available, information surveyed is often from different samples leading to poor correlation between proteome. Our goal was develop a method for concomitant cell type-specific analyses RNA protein.We utilized proximity-labeling strategy that uses biotin ligase, TurboID,...