A5026823481- Tuberculosis Research and Epidemiology
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Mycobacterium research and diagnosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Advanced Biosensing Techniques and Applications
- Chemical Safety and Risk Management
- Biochemical and Molecular Research
- Biochemical and Structural Characterization
- Occupational Health and Safety Research
- Risk and Safety Analysis
- Advanced Proteomics Techniques and Applications
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Tissue Engineering and Regenerative Medicine
- Marine Toxins and Detection Methods
- Molecular Sensors and Ion Detection
- Legume Nitrogen Fixing Symbiosis
- Quinazolinone synthesis and applications
- Immune Response and Inflammation
- Amino Acid Enzymes and Metabolism
- Environmental Toxicology and Ecotoxicology
- Animal Ecology and Behavior Studies
- Cassava research and cyanide
University of Notre Dame
2022-2024
Pepperdine University
2023
The human pathogen Mycobacterium tuberculosis typically causes lung disease but can also disseminate to other tissues. We identified a M. (Mtb) outbreak presenting with unusually high rates of extrapulmonary dissemination and bone disease. found that the causal strain carried an ancestral full-length version type VII-secreted effector EsxM rather than truncated present in modern Mtb lineages. variant exacerbated through enhancement macrophage motility, increased egress macrophages from...
A student Laboratory Safety Officer (LSO) program formalizes a position often found within academic institutions. Through clearly defined responsibilities and effective communication between an LSO their principal investigators, environmental health safety office, department administrators, this can be effectively used to establish baseline for standards. This article outlines pathways institution or strengthen program. The strength goes beyond responsibilities, where the program's...
Isotopic labeling of proteins for quantitative proteomics is a popular technique to increase sample throughput and provide improved accuracy precision relative or absolute quantitation between samples. Derivatives this are used label protein peptide N-termini selective enrichment analysis. We previously reported on method enrich quantify N-terminal acetylation in model-system pathogenic mycobacteria. Significant recent advancements silica filter-based digestion have identification bottom-up...
ABSTRACT N-terminal protein acetylation is a ubiquitous post-translational modification that impacts diverse cellular processes in higher organisms. Bacterial proteins are also N-terminally acetylated, but the mechanisms and consequences of this bacteria poorly understood. The major virulence factor EsxA (ESAT-6, early secreted antigen, 6 kDa) was one first acetylated identified bacteria. conserved mycobacterial pathogens, including Mycobacterium tuberculosis marinum , non-tubercular species...
ABSTRACT N-terminal acetylation in Mycobacterium tuberculosis is correlated with pathogenic activity. We used genomics and bottom-up proteomics to identify protein Emp1 as the sole acetyltransferase responsible for of EsxA, a known virulence factor. Using custom data analysis, we screened proteome 22 additional putative substrates Emp1.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMethyl transfers. 8. The Marcus equation and transfers between arenesulfonatesEdward S. Lewis Daniel D. HuCite this: J. Am. Chem. Soc. 1984, 106, 11, 3292–3296Publication Date (Print):May 1, 1984Publication History Published online1 May 2002Published inissue 1 1984https://pubs.acs.org/doi/10.1021/ja00323a038https://doi.org/10.1021/ja00323a038research-articleACS PublicationsRequest reuse permissionsArticle Views191Altmetric-Citations36LEARN ABOUT...
The 11 lytic transglycosylases of Pseudomonas aeruginosa have overlapping activities in the turnover cell-wall peptidoglycan. Rare lipoprotein A (RlpA) is distinct among by its use only peptidoglycan lacking peptide stems. spatial localization RlpA and interactome within P. are unknown. We employed suppression introduced amber codons at sites rlpA gene for introduction unnatural-amino-acids Ν
N-terminal protein acetylation is a ubiquitous post-translational modification that broadly impacts diverse cellular processes in higher organisms. Bacterial proteins are also N-terminally acetylated, but the mechanisms and consequences of this bacteria poorly understood. We previously quantified widespread pathogenic mycobacteria (C. R. Thompson, M. Champion, P.A. J Proteome Res 17(9): 3246-3258, 2018, https:// doi: 10.1021/acs.jproteome.8b00373). The major virulence factor EsxA (ESAT-6,...
Miniprep Assisted Proteomics (MAP) is a rapid approach to bottom-up proteomics sample preparation by adventitious binding Si-DNA minipreps. This combines the consistency of commercially produced column with low-cost in-house devices.
Laboratory safety teams (LSTs), led by graduate student and postdoctoral researchers, have been propagating across the U.S. as a bottom-up approach to improving culture in academic research laboratories. Prior COVID-19 pandemic, LSTs relied heavily on in-person projects events. Additionally, committed Champions from ranks of professionals faculty were critical their operation continued expansion. As was case for many existing systems, global crisis served an operational stress test LSTs,...
ABSTRACT The mycobacterial cell envelope is a major virulence determinant in pathogenic mycobacteria. Specific outer lipids play roles pathogenesis, modulating the immune system and promoting secretion of factors. ESX-1 (ESAT-6 system-1) conserved protein required for pathogenesis. Previous studies revealed that strains lacking lipid PDIM have impaired function during laboratory growth infection. mechanisms underlying changes are unknown. We used proteo-genetic approach to measure...
Peptidoglycan is a major constituent of the bacterial cell wall. Its integrity as polymeric edifice critical for survival and, such, it preeminent target antibiotics. The peptidoglycan dynamic crosslinked polymer that undergoes constant biosynthesis and turnover. soluble lytic transglycosylase (Slt) Pseudomonas aeruginosa periplasmic enzyme involved in this Using amber-codon-suppression methodology live bacteria, we incorporated fluorescent chromophore into structure Slt. Fluorescent...
The mycobacterial cell envelope is a major virulence determinant in pathogenic mycobacteria. Specific outer lipids play roles pathogenesis, modulating the immune system and promoting secretion of factors. ESX-1 (ESAT-6 system-1) conserved protein required for pathogenesis (1, 2). Previous studies revealed that strains lacking lipid PDIM have impaired function during laboratory growth infection (3-5). mechanisms underlying changes are unknown. We used proteo-genetic approach to measure PGL-dependent
Abstract Phagosomal lysis is a key aspect of mycobacterial infection host macrophages. Acetylation protein modification mediated enzymatically by N-acetyltransferases (NATs) that impacts bacterial pathogenesis and physiology. To identify NATs required for lytic activity, we leveraged Mycobacterium marinum, nontubercular pathogen an established model M. tuberculosis. marinum hemolysis proxy phagolytic activity. We generated strains with deletions in conserved NAT genes screened hemolytic...
Quenching digestions in proteomics prior to analysis is routine order eliminate residual protease activity. Residual activity leads overdigestion, nonspecific star-activity, and back-exchange isotopic
ABSTRACT Organs in the abdominal cavity are covered by a peritoneal membrane, which is comprised of monolayer mesothelial cells (MC). Diseases involving membrane include peritonitis, primary cancer (mesothelioma), and metastatic cancers (ovarian, pancreatic, colorectal). These diseases have gender- and/or age-related pathologies; however, impact gender age on MC not well evaluated. To address this, we identified characterized differences proteomes murine MC. Primary were isolated from young...
Sexually dimorphic phenotypes are consequential to animal survival, and this is especially apparent with defense phenotypes. Amphibians have poison glands, several lineages maintain a neurotoxin, tetrodotoxin (TTX), which largely considered derived chemical trait. However, production pathways unclear, and, as such, whether males females differentially produce or appropriate toxin concentrations not known. We evaluated the relationship between TTX concentrations, sex, morphology by collecting...