Background: A tracheal matrix scaffold decellularized by detergent-enzymatic treatment has been shown as a promising in tissue engineering. The objectives of this study were to evaluate the impact technique on extracellular integrity and characterize environment for recellularization. Methods: Brown Norway rat tracheae using modified treatment. Antigenicity cellularity monitored during processing. Glycosaminoglycan content, histoarchitecture, mechanical properties also evaluated. Matrix...

Background: Cancer screening with highly sensitive, specific biomarkers that reflect molecular phenotypic alterations is an attractive strategy for cancer control. We examined whether biomarker profiles could be used risk assessment and detection in a cohort of Chinese workers occupationally exposed to benzidine at bladder cancer. Methods: The consisted 1788 373 nonexposed workers, followed from 1991 through 1997. assayed urothelial cells voided urine samples DNA ploidy (expressed as the...

Background: The authors have previously demonstrated promising results with tissue engineered trachea in vitro using decellularized matrix scaffolds. present study aims to investigate the applicability of construct vivo. Methods: Tracheae harvested from Brown Norway rats (donor) and Lewis (recipient) were repeated detergent-enzymatic treatment cycles. Decellularized tracheal scaffolds seeded rat stem cell–derived chondrocytes externally epithelial cells internally generate a bilaminated...

Objective: Cervical burn scar contractures can be repaired using many modalities, including skin grafts, pedicled and free flaps. Although preexpanded cervical flaps provide a like-with-like reconstruction, simple advancement transfer of the often fails to achieve ideal outcomes. The authors aimed introduce method transferred in scarf-wrapping manner repair neck defects. Methods: surgery was divided into 2 stages. In first stage surgery, an expander implanted above platysma muscle on each...

Reconstructing severe cervical scar contractures (SCSC) remains a considerable challenge. This study presents novel approach to SCSC reconstruction using combination of pre-expanded bipedicled forehead and lower trapezius musculocutaneous flaps. A retrospective analysis was conducted on 25 patients who underwent this procedure between April 2004 July 2020. Sixteen received flap combined with unilateral flap; in contrast, 9 bilateral The surgery performed 4 stages. First, tissue expanders...

The expanded forehead flap based on the frontal branch of superficial temporal artery (STA-Fbr) is a versatile technique for reconstructing cervicofacial defects. aim this study was to summarize STA-Fbr harvesting patterns. Between July 2003 and 2023, 66 patients (43 males) underwent face neck defect reconstruction using STA-Fbr-based flaps. These were divided into pattern I (vascular-pedicled) II (cutaneous-pedicled) Pattern subdivided unilateral (IIa) bilateral (IIb) cutaneous-pedicled IIa...

Clinical examination alone is neither sensitive nor specific for predicting flap necrosis, so several technologies, including indocyanine green angiography, thermal imaging (using the FLIR ONE), and near-infrared spectroscopy, have been developed to supplement perfusion assessment. This study aims compare accuracy of these three methods intraoperatively clinical necrosis in a rat perforator model. The authors hypothesized that assessing oxygenation rather than direct perfusion, would yield...

Graphical Abstract Scheme of the experimental design. 1, CTA derived from donor Brown Norway rats was de-epithelialized to create DeCTA. 2, RECs were isolated syngeneic recipient Lewis rats. 3, DeCTA pre-epithelialized ReCTA. 4, In heterotopic implantation model, ReCTA subcutaneously implanted into groin area, and an adipose tissue flap pedicled by superficial epigastric blood vessels used for neovascularization. 5, orthotopic neck reconstruct a 4-ring tracheal defect.

Plastic surgeons charged with reconstructing extensive perioral defects face dual challenges of functional restoration and esthetic considerations. While forehead flaps are commonly used to reconstruct defects, in cases involving partial upper lip where normal anatomical structures preserved, traditional may compromise esthetics. This study aimed address this issue by employing bipedicled preexpanded based on the frontal branches superficial temporal artery (hereafter, “STA-bfb–based flap”)...

Elbow reconstruction is challenging for reconstructive surgeons. The purpose of this report to present the results use freestyle perforator‐based propeller flap designed from medial arm region elbow reconstruction. defects following soft tissue sarcoma resection at and posterior were repaired in two patients. dimensions 11 × 7 cm 2 10 . Two perforators identified each case using Doppler ultrasound probe arm, adjacent defect. perforator with visible pulsation was chosen as pedicle vessel,...

Mandibular distraction is a well-established method of correcting facial asymmetry in hemifacial microsomia. However, lower width remains after because its inability to increase bone thickness. Therefore, the authors used mandibular outer cortex grafting augment face following restore symmetry.From January 1997 December 2007, 21 patients with microsomia underwent unilateral followed by augmentation using graft harvested from normal side. Age at surgery ranged 7 27 years. Facial symmetry was...

This clinical study describes a reconstructive method for facial soft-tissue defects that uses the pre-expanded forehead flap and minimizes donor site morbidities.The surgery was subdivided into 3 stages. First stage, an appropriately sized expander buried underneath forehead. Second after adequate inflation of expander, based on frontal branches superficial temporal artery raised, distal portion used to reconstruct defect. The cutaneous pedicle designed near hairline. Third weeks later,...

Defects after soft tissue sarcoma resection are usually managed by myocutaneous flaps or free flaps. However, harvesting muscle will cause functional morbidities, and some regions lack reliable recipient vessel. Our purpose is to use various perforator propeller for oncologic reconstruction.Between 2008 2014, 33 were performed in 24 patients reconstruct the defects tumor trunk extremities. Fifteen underwent previously. Thirteen adjuvant radiotherapy chemotherapy. Flaps based on perforators...

Summary: Indocyanine green angiography (ICGA) is a useful tool for the visual assessment of superficial blood flow. Herein, we used ICGA to visualize perforator branches and linking vessels provide road map flap design an expanded flap. Twenty-eight expansions were planned use back-cut technique in 26 patients. was branching pattern with venous network before expander explantation. The appropriate selected, designed following axiality its branch linked by true anastomoses. vein running...

Tracheal reconstruction remains a major clinical problem owing to the need for rigid support and lining maintain patent lumen. The purpose of this study was investigate feasibility microsurgical tracheal in large animals. Two-stage attempted six dogs single-stage eight dogs. In first-stage prelamination, tubular prosthetic material lined with an abdominal fascial flap skin grafting covered rectus abdominis muscle flap. construct buried under 3 weeks, then 6-cm-long defect performed....

The lateral circumflex femoral artery system with its anatomical variations is a common source vessel for numerous thigh flaps. However, the anatomic of distally based flaps have not been well classified.Between July 2008 and 2016, 19 patients (13 men 6 women; age range, 3-58 years; mean, 27.5 years) underwent reconstruction defects around knee using Defect etiologies included malignant neoplasm (6 cases) post-burn scar contracture cases). were raised on perforating vessels originating from...

This clinical study aimed at introducing a reconstructive method for total cheek soft tissue defects using the pre-expanded medial arm flap and to maximally reduce donor site morbidities simultaneously. A retrospective review of data was performed 3 patients requiring reconstruct defects. The in primarily closed pedicle parascapular or thoracodorsal artery perforator flap. All had unilateral defect after lesion removal. Three flaps measuring 17 × 11 cm, 22 15 20 cm were separately used...

Abstract Background Free flap-mediated gene therapy in the tumor bed following surgical resection is a promising approach cancer targeted treatment of residual disease. We investigated selective killing efficacy lentivirus-mediated cytosine deaminase-thymidine kinase (CDglyTK) transplanted breast delivered into free flap by intra-artery perfusion. Methods Proliferation, apoptosis, and cell cycle rat SHZ-88 cells transfected with CD/TK were measured ganciclovir 5-flucytosine vitro. A model...