We present SUPPOSe 3Dge, a method for the detection of surfaces in volumetric fluorescence microscopy images with resolution better than diffraction limit. The works by approximating an arbitrary object surface as polyhedron formed small triangular faces, which are thought virtual point like sources signal gradient. Then, super arises from solving least square problem only on positions sources, comparing measured image gradient synthetic one built approximated and known spread function...

Spatiotemporal microscopy plays an important role in the quest for highly efficient light harvesting materials as it allows direct tracking of nanoscale transport excitons, carriers photon energy. Unfortunately, achieving high resolution both space and time often requires scanning beam spots or delay lines, limiting these techniques to specialized research groups. To overcome this problem, we introduce a novel implementation photoluminescence-detected exciton using camera composed array...

Abstract Transport phenomena like diffusion, convection, and drift play key roles in the sciences engineering disciplines. They belong to most omnipresent important nature that describe motion of entities such as mass, charge or heat. Understanding controlling these transport is crucial for a host industrial technologies applications, from cooling nuclear reactors nanoscale heat‐management semiconductor industry. For decades, macroscopic techniques have been used access parameters mobilities...

With the increasing demand for new materials light-harvesting applications, spatiotemporal microscopy techniques are receiving attention as they allow direct observation of nanoscale diffusion excitons. However, use pulsed and tightly focused laser beams generates light intensities far above those expected under sunlight illumination, leading to photodamage nonlinear effects that seriously limit accuracy applicability these techniques, especially in biological or atomically thin materials....

An alternative for commercial Raman-Optical Time Domain Reflectometry (OTDR) distributed temperature sensors is presented, where a pseudorandom pulse sequence used to increase the signal noise ratio.Additionally, deconvolution algorithm proposed eliminate distortions in reconstruction arising from real correlation properties of sensing signal.An improvement SNR factor 11 respect Raman-OTDR method at same peak power was achieved, allowing 2 m and 1.5 °C resolution 6 s measurement using 90-mW...

A new approach to the edge detection problem is presented which specially designed achieve high accuracy detection, below instrumental resolution (super resolution) in microscopy images. The method based a modified version of recently published algorithm known as SUPPOSe, performs numerical reconstruction an image superposition virtual point sources. was tested simulated and experimental optical images compared standard Laplacian Gaussian algorithm, showing huge differences when size object...

We present gSUPPOSe, a novel, to the best of our knowledge, gradient-based implementation SUPPOSe algorithm that we have developed for localization single emitters. study performance gSUPPOSe and compressed sensing STORM (CS-STORM) on simulations single-molecule microscopy (SMLM) images at different fluorophore densities in wide range signal-to-noise ratio conditions. also combination these methods with prior image denoising by means deep convolutional network. Our results show can address...

A confocal Raman microscopy technique has been designed and demonstrated that measures the temperature rise profile in microfluidic devices. The system is based deformation of water peak assigned to O-H stretching at 3400 cm⁻¹ occurs with keeping an isosbectic point 3425 . Hence two photon counting detectors sample emission each side are used monitor temperature. Using a detection scheme spatial resolution microscope can be obtained map within small structure noninvasive manner....

A novel method for noninvasive, three-dimensional temperature characterization in microfluidic devices is presented. specially designed confocal microscope was built and used to measure water by sensing the Raman spectrum variations of liquid. This achieved splitting isosbestic point detecting it with two photon counters. The difference between signals each detector divided their sum shows a linear dependence temperature. fiber-coupled laser beam pump sample 25 mW optical power at 405 nm....

Edge detection in images is a key problem quantitative cell biology, nanophotonics and other areas, but the edge-detection algorithms that have been developed suffer from serious limitations precision. This year, we presented computational method for super-resolution edge single standard opens possibility of detecting shapes objects with features much smaller than microscope’s point spread function.

No English translation of this document was provided by the author or conference organizers.

The deconvolution of sparse objects images is analyzed after using the SUPPOSe algorithm and its combination with artificial intelligence denoising, showing resolutions under 100nm high noise levels.

Este trabajo intenta reconstruir la lectura de Spinoza que realiza Carl Schmitt. Nuestra hipótesis que, en el contexto su apoyo al nazismo, Schmitt retoma las críticas –con un cariz más metafísico político ahora– sostenía sobre liberalismo Weimar, cual no casualmente tuvo una suerte renacimiento y reconocimiento académico reivindicativo referencias intelectuales época. Asimismo, se desarrollan particularidades recepción schmittiana Spinoza, entre ellas influencia del Leo Strauss pleno...