Modification of material surfaces aimed at bestowing them with antimicrobial properties is a promising approach in the development new biomaterials. Antimicrobial peptides (AMPs) are an attractive alternative to conventional antibiotics because lack toxicity, inherently high selectivity, and absence immune response. As mode action AMP cathelin LL37 formation pores disruption microbial membrane, purpose present study was develop test method covalent immobilization on titanium surface. The...

Abstract Direct surface modification of biodegradable polycaprolactone (PCL) was performed without the necessity synthesis functionisable co-polymers. An easy-to-perform three-step procedure consisting amination, reaction with hetero-bifunctional cross-linkers and conjugation an RGD-motif-containing peptide used to modify polymer films improve attachment endothelial cells. The biological activity modified surfaces assessed by estimating microvascular cell attachment. Covalent coating RGD...

Functional coatings on titanium vascular stents and endosseous dental implants could probably enhance endothelial cell (EC) adhesion activity with a shortening of the wound healing time an increase peri-implant angiogenesis during early bone formation. Therefore, role structure linear cyclic adhesive peptides Arg-Gly-Asp (l-RGD c-RGD) differently pre-treated (Ti) surfaces (untreated, silanised vs. functionalised l- c-RGD peptides) EC coverage proliferation was evaluated. After 24 h after 3...

Platelet releasate has been shown to promote osteogenetic cell proliferation and differentiation. Topography chemistry of biomaterials have high impact on platelet activation. More specifically, the bioactive adhesive peptide sequence Arg-Gly-Asp (RGD) triggers activation mediated by α(IIb) β(3) integrin receptor. Accordingly, topographical, chemical biomimetical (immobilized RGD peptide) modifications titanium (Ti) surfaces may enhance early bony healing implants. Therefore, aim study was...

Many biomaterials used for tissue engineering applications lack cell-adhesiveness and, in addition, are prone to nonspecific adsorption of proteins. This is especially important blood-contacting devices such as vascular grafts and valves where appropriate surface properties should inhibit the initial attachment platelets promote endothelial cell colonization. As a consequence, long-term outcome implants would be improved need anticoagulation therapy could reduced or even abolished....

Polytetrafluoroethylene (PTFE), a frequently utilized polymer for the fabrication of synthetic vascular grafts, was surface-modified by means wet-chemical process. The inherently non-cell-adhesive does not support cellular attachment, prerequisite endothelialization luminal surface grafts in small diameter applications. To impart material with cell-adhesive properties treatment sodium-naphthalene provided basis subsequent immobilization adhesion promoting RGD-peptide using hydroxy- and...

In recent years, the synthetic polymer polyetheretherketone (PEEK) has increasingly been used in a number of orthopedic implementations, due to its excellent mechanical properties, bioinertness, and chemical resistance. For vivo applications, surface PEEK, which does not naturally support cell adhesion, be modified improve tissue integration. present work we demonstrate novel wet‐chemical modification PEEK modify surface, enabling covalent grafting cell‐adhesive RGD‐peptide. Modification was...

Scaffold production for tissue engineering was demonstrated by means of a hot compression molding technique and subsequent particulate leaching. The utilization spherical salt particles as the pore-forming agent ensured complete interconnectivity porous structure. This method obviated use potentially toxic organic solvents. To overcome inherent non-cell-adhesive properties hydrophobic polymer polycaprolactone (PCL) surface activation with diamine performed, followed covalent immobilization...

Endowing materials surface with cell-adhesive properties is a common strategy in biomaterial research and tissue engineering. This particularly interesting for already approved polymers that have long standing use medicine because these are well characterized legal issues associated the introduction of newly synthesized may be avoided. Polytetrafluoroethylene (PTFE) one most frequently employed manufacturing vascular grafts but polymer lacks cell adhesion promoting features....

Background: Whole human saliva (WHS) and its high molecular weight mucin constituent (Muc) inhibit fibroblast attachment might influence periodontal peri‐implant wound healing. The aim of this work was to study the potential role glycosylation Muc in attachment‐inhibiting property examine vitro effect WHS on epithelial cell attachment. Methods: isolated from by CsCl density gradient ultracentrifugation; covalently immobilized polystyrene; subjected enzymic digestion N‐glycanase, O‐glycanase,...

Abstract Polyvinyl chloride (PVC) is one of the most frequently used polymers for manufacturing medical devices. Limitations its usage are based upon unfavorable surface properties polymer including hydrophobicity and lack functionalities in order to increase versatility. To address this issue, wet chemical modification PVC was performed through amination using bifunctional compound ethylene diamine. The reaction conducted achieve maximum while leaving bulk material unaffected. initial...

Abstract Plasma polymerized films of allylamine were surface derivatized in subsequent wet chemical processes using a diepoxy‐PEG linker to covalently bind different biomolecules that are known influence cell adhesion. The initial attachment human umbilical vein endothelial cells (HUVECs) was studied on (i) unmodified PTFE, (ii) PTFE modified with layer plasma (pp‐AA), and (iii) pp‐AA which further adhesion molecules coupled the functional via molecule. Statistical analysis data initially...

Abstract Polydimethylsiloxane (PDMS) is an elastomeric polymer frequently used as implant material, for flexible tubing and in microfluidic devices. The pronounced hydrophobic surface of this unique material impedes many applications where a good wetting behavior required. Consequentially, various ways modifications have been to introduce new properties. Plasma treatment the most popular technique respect, but not generally applicable, especially if hardly accessible surfaces are be...

Endothelialization of blood contacting implants, e.g., vascular stents, is regarded as a prerequisite for an improved performance in terms minimizing thrombogenicity and the inhibition restenosis. Commonly used materials, such Ti-based alloys, can be surface-modified order to improve endothelial cell (EC) colonization well reduce platelet adhesion. Standard modification techniques involve silanization are laborious time-consuming. We propose novel single-step procedure based on...

Endowing materials surface with cell-adhesive properties is a common strategy in biomaterial research and tissue engineering. This particularly interesting for already approved polymers that have long standing use medicine because these are well characterized legal issues associated the introduction of newly synthesized may be avoided. Polytetrafluoroethylene (PTFE) one most frequently employed manufacturing vascular grafts but polymer lacks cell adhesion promoting features....

Tissue engineering concepts, which are concerned with the attachment and growth of specific cell types, frequently employ immobilized ligands that interact preferentially types interest. Creating multicellular grafts such as heart valves calls for scaffolds spatial control over different cells involved. Cardiac mainly constituted out two endothelial valvular interstitial cells. To have where type can be attracted would enable targeted settlement contributing to first step an engineered...

Objectives: Thrombosis is one major cause of poor patency synthetic vascular grafts. Amongst others, the latter promoted by absence endothelialization. In addition, commonly used prosthetic materials - e.g. polytetrafluoroethylene (PTFE) are prone to bacterial biofilm formation. The present study aims at development a PTFE-surface modification offering antifouling as well endothelial cell-adhesive properties. Polyethyleneglycol (PEG) was spacer molecule simultaneously providing effects....

Infections caused by viruses are difficult to treat due their life cycle, which depends on the replication machinery of respective host cells. Commonly used antiviral strategies based upon application of, e.g., entry inhibitors and other compounds that interfere with virus replication. Besides possible side effects, rapid occurrence viral resistance poses a great challenge. Antimicrobial peptides (AMPs), as component innate immunity, able kill bacteria fungi and, in addition, may inactivate...

The surface modification of polyvinylidene difluoride (PVDF) for various biomedical uses is notoriously hampered by the chemical inertness polymer. A wet approach aiming at covalently grafting biomolecules was demonstrated means an elimination reaction fluorine from polymer backbone followed subsequent steps. Exemplified as a possible biological application, coupling peptide REDV rendered material adhesive endothelial cells while adhesion thrombocytes dramatically reduced.