A5030950537- Ion channel regulation and function
- Cardiomyopathy and Myosin Studies
- Cancer Immunotherapy and Biomarkers
- Congenital heart defects research
- Cardiac electrophysiology and arrhythmias
- Hormonal Regulation and Hypertension
- Neurobiology and Insect Physiology Research
- Sepsis Diagnosis and Treatment
- Cardiovascular Function and Risk Factors
- Adenosine and Purinergic Signaling
- Ion Channels and Receptors
- Cardiac Structural Anomalies and Repair
- Cardiac Fibrosis and Remodeling
- Physiological and biochemical adaptations
- Chemotherapy-induced cardiotoxicity and mitigation
- CAR-T cell therapy research
- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- Peptidase Inhibition and Analysis
- Sperm and Testicular Function
- Receptor Mechanisms and Signaling
- Ion Transport and Channel Regulation
- Genetics, Aging, and Longevity in Model Organisms
- Neuroscience and Neural Engineering
- Lipid Membrane Structure and Behavior
Inserm
2012-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Aix-Marseille Université
2012-2024
Theories and Approaches of Genomic Complexity
2012-2023
Délégation Provence et Corse
2022
Unité de recherche sur les maladies cardiovasculaires et métaboliques
2022
Assistance Publique Hôpitaux de Marseille
2018-2021
University College London
2021
Centre National de la Recherche Scientifique
1997-2012
Institut de Biologie du Développement Marseille
2003-2012
Background Cardiac MRI features are not well-defined in immune checkpoint inhibitor (ICI)–induced myocarditis (ICI-M), a severe complication of ICI therapy patients with cancer. Purpose To analyze the cardiac ICI-M and to explore their prognostic value major adverse cardiovascular events (MACE). Materials Methods In this retrospective study from May 2017 January 2020, findings (including late gadolinium enhancement [LGE], T1 T2 mapping, extracellular volume fraction [ECV] z scores) were...
Neuroendocrine differentiation of prostate epithelial cells is usually associated with an increased aggressivity and invasiveness tumors a poor prognosis. However, the molecular mechanisms involved in this process remain poorly understood. We have investigated possible expression voltage-gated calcium channels human cancer LNCaP their modulation during neuroendocrine differentiation. A small proportion undifferentiated displayed voltage-dependent current. This current density were...
Journal Article Aldosterone increases T-type calcium channel expression and in vitro beating frequency neonatal rat cardiomyocytes Get access Nathalie Lalevé, Lalevé aDivision of Endocrinology Diabetology, Department Internal Medicine, University Hospital, CH-1211 Geneva 14, Switzerland Search for other works by this author on: Oxford Academic PubMed Google Scholar Michela C. Rebsamen, Rebsamen SwitzerlandbLaboratory Clinical Chemistry, Pathology, Stéphanie Barrère-Lemaire, Barrère-Lemaire...
Cardiac hypertrophy underlies arrhythmias and sudden death, for which mineralocorticoid receptor (MR) activity has recently been implicated. We sought to establish the sequence of ionic events that link initiating insult MR development.Using whole-cell, patch-clamp quantitative reverse transcription-polymerase chain reaction techniques on right ventricular myocytes a myocardial infarction (MI) rat model, we examined cellular response over time. One week after MI, no sign was found, but...
Background Myocarditis is a rare but life-threatening adverse event of cancer treatments with immune checkpoint inhibitors (ICIs). Recent guidelines recommend the use high doses corticosteroids as first-line treatment, followed by intensified immunosuppressive therapy (IIST) in case unfavorable evolution. However, this strategy empirical, and no studies have specifically addressed issue. Therefore, we aimed to investigate compare clinical course, management outcome ICI-induced myocarditis...
Introduction: Amiodarone (AM) is a highly effective antiarrhythmic agent used in the management of both atrial and ventricular arrhythmias. Its noniodinated analogue dronedarone (SR) may have fewer side effects than AM. In this study, we compared AM SR on sodium current I Na human myocytes. Methods Results: was studied with whole‐cell, patch clamp technique. Both induced dose‐dependent inhibition recorded at −40 mV from holding potential −100 mV. inhibited by 41%± 11% (n = 4) 3 μM, 80%± 7%...
Cardiac aging is a complex process, which influenced by both environmental and genetic factors. Deciphering the mechanisms involved in heart senescence therefore requires identifying molecular pathways that are affected age controlled conditions. We describe functional genomic investigation of control cardiac Drosophila. Molecular signatures were identified differential transcriptome analysis followed detailed bio-informatic analysis. This approach implicated JNK/dJun pathway transcription...
Left ventricular non-compaction (LVNC) is a rare cardiomyopathy associated with hypertrabeculated phenotype and large spectrum of symptoms. It still unclear whether LVNC results from defect trabeculae development the mechanistic basis that underlies varying severity this pathology unknown. To investigate these issues, we inactivated cardiac transcription factor Nkx2-5 in trabecular myocardium at different stages morphogenesis using an inducible Cx40-creERT2 allele. Conditional deletion...
Mechanotransduction modulates cellular functions as diverse migration, proliferation, differentiation, and apoptosis. It is crucial for organ development homeostasis leads to pathologies when defective. However, despite considerable efforts made in the past, molecular basis of mechanotransduction remains poorly understood. Here, we have investigated genetic Drosophila. We show that fly heart senses responds mechanical forces by regulating cardiac activity. In particular, pauses activity are...
Objective: The aim of the study was to assess value in vivo T2 measurements noninvasively quantify myocardial fibrosis diabetic mice at 11.75 T. Diabetic cardiomyopathy is characterized by extracellular matrix alteration and microcirculation impairment. These conditions might provide electrical heterogeneity, which a substrate for arrhythmogenesis. T1 mapping has been proposed diffuse cardiac diseases but several limitations. measurement may represent an alternative quantification high...
To investigate any gender effect of the beta-1 adrenergic blocker, landiolol, on cardiac performance and energy metabolism in septic rats, to explore expression genes proteins involved this process.Randomized animal study.University research laboratory.Male female Wistar rats.One hour after cecal ligation puncture, male rats were randomly allocated following groups: sham male, puncture + landiolol female, female. Cardiac MRI was carried out 18 hours assess vivo function. Ex function...
We have applied the whole-cell configuration of patchclamp technique to investigate calcium currents in Sertoli cells from immature rat testis primary culture. Cesium-filled pipettes were used block potassium currents. In presence 10 mM extracellular (Ca2+), depolarizations elicited transient inward range -70 +10 mV with a maximal amplitude -1.3 pA/pF at -30 mV. This component activated -20 (V0.s = -42 mV) and inactivated -75 -45 (Vo.s -61 mV). Currents not modified when barium (Ba2+) was...
Abstract Although epidemiologic research has demonstrated significant differences in incidence and outcomes of sepsis according to sex, their underlying biological mechanisms are poorly understood. Here, we studied the influence hormonal status by comparing vivo cardiac performances measured MRI non-ovariectomized ovariectomized septic female rats. Control rats were randomly allocated following groups: sham, plus landiolol. Sepsis was induced caecum ligation punction (CLP). Landiolol, a...
The ability of ATP and FSH to induce intracellular calcium [Ca2+]i changes in Sertoli cells is imperfectly understood reports are conflicting. We have applied the single-cell microfluorometry technique with probe indo-1 investigate individual cultured cells. When were exposed ATP, cAMP, FSH, a fast biphasic increase was obtained 100%, 70%, 56% cells, respectively. Caffeine did not activate Ca2+ mobilization, while thapsigargin suppressed peak response. External free-EGTA buffer plateau...
The ventricular conduction or His-Purkinje system (VCS) mediates the rapid propagation and precise delivery of electrical activity essential for synchronization heartbeats. Mutations in transcription factor Nkx2-5 have been implicated a high prevalence developing defects arrhythmias with age. heterozygous mutant mice reproduce human phenotypes associated hypoplastic resulting from defective patterning Purkinje fiber network during development. Here, we investigated role mature VCS...
Angiotensin II and extracellular potassium stimulate aldosterone production in adrenal glomerulosa cells by mobilizing the calcium messenger system. This response requires influx across plasma membrane, followed uptake into mitochondria. It has been proposed that is transported to mitochondria via lumen of endoplasmic reticulum, acting as a kind intracellular pipeline. hypothesis tested present study measuring intramitochondrial variations H295R with new fluorescent probe, ratiometric...
In solid cancer surgery, oxidative stress (OS) is of particular interest because both the malignancy itself and surgical intervention are major contributors to modulation OS levels. This study aimed investigate effects surgery on Because often accompanied by intense cell death, specifically focused pathway leading uric acid formation, (i.e. adenosine deaminase (ADA) xanthine oxidase (XO). Ische-mia-modified albumin (IMA), a recognized marker stress, plasma redox poten-tial (RP) were also...