A5090440036- Aldose Reductase and Taurine
- Cancer-related gene regulation
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer Mechanisms and Therapy
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
Xiamen University
2019-2021
ABSTRACT Autophagy is considered to be an important switch for facilitating normal malignant cell transformation during colorectal cancer development. Consistent with other reports, we found that the membrane receptor Neuropilin1 (NRP1) greatly upregulated in colon cells underwent autophagy upon glucose deprivation. However, mechanism underlying NRP1 regulation of unknown. We knockdown inhibits and largely upregulates expression aldo-keto reductase family 1 B10 (AKR1B10). Moreover,...
目的：探讨去甲基化药物5-杂氮-2＇-脱氧胞苷（5-Aza-CdR）在裸鼠体内诱导HepG2细胞T-cadherin的表达,及其对HepG2种植瘤生长的抑制作用. 方法：皮下接种法建立HepG2细胞裸鼠种植瘤模型,随机分为实验组（11只）和对照组（10只）.实验组裸鼠给予5-Aza-CdR注射,对照组裸鼠注射等量PBS.4wk后处死裸鼠,观察种植瘤的生长情况,用RT-PCR技术及免疫组织化学技术检测T-cadherin mRNA及蛋白的表达. 结果：用药4wk后,实验组肿瘤组织的T-cadherin mRNA的表达水平显著高于对照组（t=6.613,P〈0.05）;对照组HepG2细胞几乎检测不到T-cadherin蛋白表达,而实验组裸鼠种植瘤细胞膜上可检测到T-cadherin蛋白表达;实验组肿瘤的平均体积明显小于对照组肿瘤平均体积（t=2.337,P=0.025）....
Abstract Aldo-keto reductase family 1, member B10 (AKR1B10) has been reported to be involved in tumorigenesis of various cancer. In our studies, we evaluated the relationship between AKR1B10 expression and clinicopathological characteristics colon cancer showed that was significantly correlated with TNM stage clinical It colorectal is closely associated chronic inflammation underlying molecular mechanisms are still elusive. Here found knockdown decreased inflammatory cytokines, IL1α IL6,...