A5053036995- Cell Adhesion Molecules Research
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Sepsis Diagnosis and Treatment
- Platelet Disorders and Treatments
- Venous Thromboembolism Diagnosis and Management
- Heat shock proteins research
- Connexins and lens biology
- Cancer-related molecular mechanisms research
- Sleep and Work-Related Fatigue
- Intensive Care Unit Cognitive Disorders
- Workplace Health and Well-being
- Blood Coagulation and Thrombosis Mechanisms
- Immune cells in cancer
- Adipose Tissue and Metabolism
- IL-33, ST2, and ILC Pathways
- COVID-19 Clinical Research Studies
- Acute Ischemic Stroke Management
- Advanced biosensing and bioanalysis techniques
- Respiratory Support and Mechanisms
- Cancer, Stress, Anesthesia, and Immune Response
Mie University
2015-2025
Boston Children's Hospital
2009-2014
Harvard University
2003-2014
Boston Children's Museum
2013
Boston University
2011
Children's Hospital
2011
Beth Israel Deaconess Medical Center
2008
Boston Biomedical Research Institute
2008
California Institute for Biomedical Research
2007
Massachusetts General Hospital
2003
Cyclin D1 (CyD1) is a pivotal cell cycle–regulatory molecule and well-studied therapeutic target for cancer. Although CyD1 also strongly up-regulated at sites of inflammation, its exact roles in this context remain uncharacterized. To address question, we developed strategy selectively silencing leukocytes vivo. Targeted stabilized nanoparticles (tsNPs) were loaded with CyD1–small interfering RNA (siRNA). Antibodies to β 7 integrin (β I) then used specific leukocyte subsets involved gut...
Silencing gene expression by RNAi is a powerful method for exploring function and validating drug targets potentially therapy. Lymphocytes other primary blood cells are resistant to lipid-based transfection in vitro difficult target vivo. We show here that antibody-protamine fusion proteins targeting the human integrin lymphocyte function-associated antigen-1 (LFA-1) efficiently deliver siRNAs specifically induce silencing lymphocytes, monocytes, dendritic cells. Moreover, protein...
RNA interference (RNAi)–mediated knockdown of gene expression offers a novel treatment strategy for human immunodeficiency virus (HIV) infection. However, the major hurdle clinical use is practical small interfering (siRNA) delivery to multiple immune cell types important in viral pathogenesis. We have developed immunoliposome method targeting lymphocyte function–associated antigen-1 (LFA-1) integrin expressed on all leukocytes and evaluated it systemic siRNA humanized mouse model. show that...
Abstract The lymphatic system is essential for the generation of immune responses by facilitating cell trafficking to lymph nodes. Dendritic cells (DCs), most potent APCs, exit tissues via vessels, but mechanisms interaction between DCs and endothelium potential implications these interactions are poorly understood. In this study, we demonstrate that endothelial (LECs) modulate maturation function DCs. Direct contact human monocyte-derived with an inflamed, TNF-α-stimulated reduced...
The spike glycoprotein attached to the envelope of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) binds and exploits angiotensin-converting enzyme (ACE2) as an entry receptor infect pulmonary epithelial cells. A subset integrins that recognize arginyl–glycyl–aspartic acid (RGD) sequence in cognate ligands has been predicted silico bind and, thereby, be exploited for viral infection. Here, we show experimental evidence β1 predominantly expressed on human cell lines primary mouse...
Abstract Endothelial monolayers have shown the ability to signal each other through gap junctions. Gap junction-mediated cell-cell interactions been implicated in modulation of endothelial cell functions during vascular inflammation. Inflammatory mediators alter mechanical properties cells, although exact role junctions this process remains unclear. Here, we sought study regulation stiffness, an important physical feature that is associated with many pathologies. The cellular stiffness...
Sepsis is a systemic inflammatory disorder induced by dysregulated immune response to infection resulting in dysfunction of multiple critical organs, including the intestines. Previous studies have reported contrasting results regarding abilities exosomes circulating blood sepsis mice and patients either promote or suppress inflammation. Little known about how gut epithelial cell-derived released intestinal luminal space during affect mucosal To study this question, we isolated extracellular...
Understanding allostery may serve to both elucidate mechanisms of protein regulation and provide a basis for engineering active mutants. Herein we describe directed evolution applied the integrin alpha(L) inserted domain studying by using yeast surface display system. Many hot spots activation are identified, some single mutants exhibit remarkable increases 10,000-fold in affinity physiological ligand, intercellular adhesion molecule-1. The location activating mutations traces out an...
The skin is an immune organ that contains innate and acquired systems thus able to respond exogenous stimuli producing large amount of proinflammatory cytokines including IL-1 family members. role the epidermal not limited initiation local inflammatory responses, but also induction systemic inflammation. However, association persistent release members from severe diseases such as psoriasis, epidermolysis bullosa, atopic dermatitis, blistering desmoglein-1 deficiency syndrome with in organs...
Abstract Inflammatory bowel diseases (IBDs) accompany a critical loss of the frontline barrier function that is achieved primarily by intestinal epithelial cells (IECs). Although gene-regulation pathways underlying these host-defense roles IECs presumably are deranged during IBD pathogenesis, quantitative and qualitative alterations posttranscriptional regulators such as microRNAs (miRNAs) within largely remain to be defined. We aimed uncover regulatory miRNA–target gene relationships arise...
Background Environmental and endogenous factors under genetic predisposition are considered to initiate the human intervertebral disc (IVD) degeneration. DNA methylation is an essential mechanism ensure cell-specific gene expression for normal development tissue stability. Aberrant epigenetic alterations play a pivotal role in several diseases, including osteoarthritis. However, alternations, methylation, IVD degeneration have not been evaluated. The purpose of this study was comprehensively...
Although thrombosis in coronavirus disease 2019 (COVID-19) infection has attracted attention, the mechanism underlying its development remains unclear. The relationship between platelet activation and severity of COVID-19 was compared with that involving other infections. Plasma soluble C-type lectin-like receptor 2 (sCLEC-2) levels were measured 46 patients 127 plasma sCLEC-2 {median (25th, 75th percentile), 489 (355, 668) ng/L} significantly higher (p < 0.001) comparison to suffering from...
The incidence of delirium in patients with burns receiving treatment the intensive care unit (ICU) is high, reaching up to 77%, and has been associated increased mortality rates. Therefore, early identification at high risk onset essential for improving strategies. This study aimed create a machine learning model predicting during their ICU stay using patient data from first day admission identify predictive factors burns. focused on 82 aged ≥18 years who were admitted Mie University...