A5075493048- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Erythrocyte Function and Pathophysiology
- Enzyme Structure and Function
- Diet, Metabolism, and Disease
- Biochemical and Molecular Research
- Pancreatic function and diabetes
- Nuclear Physics and Applications
- Carbohydrate Chemistry and Synthesis
- Porphyrin and Phthalocyanine Chemistry
- Nuclear reactor physics and engineering
- Laser-induced spectroscopy and plasma
- Neonatal Health and Biochemistry
- Metabolism, Diabetes, and Cancer
- Calcium signaling and nucleotide metabolism
- Amino Acid Enzymes and Metabolism
- Nuclear Materials and Properties
- Crystallography and molecular interactions
- Fiber-reinforced polymer composites
- Metal-Catalyzed Oxygenation Mechanisms
- Cellular transport and secretion
- Radiation Detection and Scintillator Technologies
- Protein Structure and Dynamics
- Folate and B Vitamins Research
- Toxin Mechanisms and Immunotoxins
Centre National de la Recherche Scientifique
2009-2024
Institut de Chimie Moléculaire et des Matériaux d'Orsay
2009-2024
Université Paris-Saclay
2018-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2006-2019
CEA Paris-Saclay
2006-2019
Direction des énergies
2007-2018
Université Paris-Sud
2003-2018
Laboratoire de Chimie
2004-2016
Inserm
2007
Université Paris Cité
1999-2007
The pentose metabolism of Archaea is largely unknown. Here, we have employed an integrated genomics approach including DNA microarray and proteomics analyses to elucidate the catabolic pathway for D-arabinose in Sulfolobus solfataricus. During growth on this sugar, a small set genes appeared be differentially expressed compared with D-glucose. These were heterologously overexpressed Escherichia coli, recombinant proteins purified biochemically studied. This showed that oxidized...
Phosphoglucose isomerase (PGI; E.C. 5.3.1.9) catalyzes the second step in glycolysis, interconversion of D-glucose-6-phosphate and D-fructose-6-phosphate. We determined X-ray crystal structure rabbit PGI complexed with a competitive inhibitor activity, 5-phospho-D-arabinonate (5PAA), at 1.9 A resolution. 5PAA is better mimic proposed cis-enediol(ate) intermediate than 6-phospho-D-gluconate, which was used previously reported PGI. The orientation bound enzyme active site predicts that residue...
Abstract Type I phosphomannose isomerase (PMI) is a Zn‐dependent metalloenzyme involved in the isomerization of D ‐fructose 6‐phosphate to ‐mannose 6‐phosphate. One our laboratories has recently designed and synthesized 5‐phospho‐ ‐arabinonohydroxamate (5PAH), an inhibitor endowed with nanomolar affinity for PMI (Roux et al., Biochemistry 2004, 43, 2926). By contrast, ‐arabinonate (5PAA), which hydroxamate moiety replaced by carboxylate one, devoid inhibitory potency. Subsequent biochemical...
Phosphoglucose isomerase (EC 5.3.1.9 ) catalyzes the second step in glycolysis, reversible isomerization of d -glucose 6-phosphate to -fructose 6-phosphate. The reaction mechanism involves acid-base catalysis with proton transfer and proceeds through a cis -enediol(ate) intermediate. 5-Phospho- -arabinonohydroxamic acid (5PAH) is synthetic small molecule that resembles intermediate, differing only it has nitrogen atom place C1. Hence, 5PAH best inhibitor reported date K i 2 × 10 −7 M. Here...
Using polarizable molecular mechanics, a recent study [de Courcy et al. J. Am. Chem. Soc., 2010, 132, 3312] has compared the relative energy balances of five competing inhibitors FAK kinase. It showed that inclusion structural water molecules was indispensable for an ordering consistent with experimental one. This approach is now extended to compare binding affinities four active site ligands Type I Zn-metalloenzyme phosphomannose isomerase (PMI) from Candida albicans. The first three ones...
Nuclear heating inside an MTR reactor has to be known in order able control samples temperature during irradiation experiments. An R&D program been carried out at CEA design a new type of in-core calorimetric system. This development, started 2002, for main objective manufacture calorimeter suitable monitoring nuclear the 70 MWth OSIRIS material testing operated by CEA's Energy Division Saclay research center. innovative probe, associated specific handling system, designed provide access...
Ribose-5-phosphate isomerase (Rpi), an important enzyme in the pentose phosphate pathway, catalyzes interconversion of ribulose 5-phosphate and ribose 5-phosphate. Two unrelated isomerases have been identified, RpiA RpiB, with different structures active site residues. The reaction catalyzed by both enzymes is thought to proceed via a high energy enediolate intermediate, analogy other carbohydrate isomerases. Here we present studies RpiB from Mycobacterium tuberculosis together small...
Trypanosoma cruzi, the human parasite that causes Chagas disease, contains a functional pentose phosphate pathway, probably essential for protection against oxidative stress and also R5P (ribose 5-phosphate) production nucleotide synthesis. The haploid genome of CL Brener clone one gene coding Type B Rpi 5-phosphate isomerase), but genes encoding A Rpis, most frequent in eukaryotes, seem to be absent. RpiB enzyme was expressed Escherichia coli as poly-His tagged active dimeric protein, which...
Abstract Type I phosphomannose isomerases (PMIs) are zinc‐dependent metalloenzymes involved in the reversible isomerization of D ‐mannose 6‐phosphate (M6P) and ‐fructose (F6P). 5‐Phospho‐ ‐arabinonohydroxamic acid (5PAH), an inhibitor endowed with nanomolar affinity for yeast (Type I) Pseudomonas aeruginosa II) PMIs (Roux et al., Biochemistry 2004; 43:2926–2934), strongly inhibits human PMI (for which we report improved expression purification procedure), as well Escherichia coli PMI. Its K...
The riboflavin biosynthesis pathway has been shown to be essential in many pathogens and is absent humans. Therefore, enzymes involved synthesis are considered as potential antibacterial drug targets. enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBPS) catalyzes one of the two committed steps converts d-ribulose 5-phosphate (Ru5P) l-3,4-dihydroxy-2-butanone 4-phosphate formate. Moreover, DHBPS indispensable for Mycobacterium, Salmonella, Helicobacter species. Despite essentiality...
H. Irving, J. V. R. Smit and L. Salmon, Analyst, 1957, 82, 549 DOI: 10.1039/AN9578200549
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis, characterization, and acid-induced decomposition of the .alpha.- .beta.-diastereomers (2-hydroxyethyl)- (2-alkoxyethyl)cobalamins cobinamidesKenneth L. Brown, Laurent Salmon, John A. KirbyCite this: Organometallics 1992, 11, 1, 422–432Publication Date (Print):January 1992Publication History Published online1 May 2002Published inissue 1 January...
The phosphomannose isomerases (PMI) comprise three families of proteins: type I, II, and III PMIs. Members all catalyze the reversible isomerization d-mannose 6-phosphate (M6P) d-fructose (F6P) but share little or no sequence identity. Because (1) PMIs are essential for survival several microorganisms, including yeasts bacteria, (2) PMI enzymes from pathogens do not significant identity to human protein, have been considered as potential therapeutic targets. Elucidation catalytic regulatory...