No AccessJournal of UrologyAdult Urology1 Sep 2018Epstein-Barr Virus as a Potential Etiology Persistent Bladder Inflammation in Human Interstitial Cystitis/Bladder Pain Syndrome Jia-Fong Jhang, Yung-Hsiang Hsu, Chih-Wen Peng, Yuan-Hong Jiang, Han-Chen Ho, and Hann-Chorng Kuo JhangJia-Fong Jhang Department Urology, Buddhist Tzu Chi General Hospital University, Hualien, Taiwan , HsuYung-Hsiang Hsu Pathology, PengChih-Wen Peng Institute Medical Sciences Life Sciences, JiangYuan-Hong Jiang...

ABSTRACT Members of the Closteroviridae and Potyviridae families plant positive-strand RNA viruses encode one or two papain-like leader proteinases. In addition to a C-terminal proteolytic domain, each these proteinases possesses nonproteolytic N-terminal domain. We compared functions several using gene swapping approach. The proteinase (L-Pro) Beet yellows virus (BYV; closterovirus) was replaced with L1 L2 Citrus tristeza (CTV; another closterovirus), P-Pro Lettuce infectious (LIYV;...

Significance Epstein–Barr Virus (EBV) genome persistence and expression in human cells can result lymphocyte or epithelial cell malignancies. EBV gene depends on the encoded virus nuclear antigen 1 (EBNA1) protein binding to cognate sites episomes. Search for a necessary EBNA1 interaction with episomes identified low-level nucleolin (NCL) association EBNA1. Despite stable association, NCL shRNA knockdowns revealed wild-type NCL, but not an ATP site point mutant be critical episomes,...

Epstein-Barr Virus (EBV) is an oncogenic γ-herpesvirus that capably establishes both latent and lytic modes of infection in host cells causes malignant diseases humans. Nuclear antigen 2 (EBNA2)-mediated transcription cellular viral genes essential for the establishment maintenance EBV latency program B lymphocytes. Here, we employed a protein affinity pull-down LC-MS/MS analysis to identify nucleophosmin (NPM1) as one proteins bound EBNA2. Additionally, specific domains are responsible...

Epstein-Barr Virus (EBV) Nuclear Antigen 1 (EBNA1)-mediated origin of plasmid replication (oriP) DNA episome maintenance is essential for EBV-mediated tumorigenesis. We have now found that EBNA1 binds to Ribosome Protein L4 (RPL4). RPL4 shRNA knockdown decreased activation an oriP luciferase reporter, binding in lymphoblastoid cell lines, and EBV genome number per line. infection increased expression redistributed nuclei. Nucleolin (NCL) were a scaffold EBNA1-induced complex. The N terminus...

Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) plays an important role in driving immortalization of EBV-infected B cells through regulating the expression many viral and cellular genes. We report a structural study tumor suppressor BS69/ZMYND11 C-terminal region, comprised tandem coiled-coil-MYND domains (BS69CC-MYND), complex with EBNA2 peptide containing PXLXP motif. The coiled-coil domain BS69 self-associates to bring two separate MYND close proximity, thereby enhancing MYND-EBNA2...

Our previous study showed that the Epstein–Barr virus (EBV) may be etiology for some patients with interstitial cystitis/bladder pain syndrome (IC/BPS); hence, current aimed to investigate urinary viral spectrum in IC/BPS and clinical efficacy of valacyclovir. Twenty-eight were prospectively enrolled valacyclovir 500 mg twice a day 4 weeks. Urine samples collected from 30 controls. The primary outcome was difference visual analog scale (VAS) score, secondary outcomes included changes...

The 66-kDa leader proteinase (L-Pro) of the Beet yellows virus (BYV) possesses a nonconserved N-terminal domain and conserved, papain-like C-terminal domain. Previous work revealed that functions in RNA amplification, whereas is required for autoproteolysis. Alanine-scanning mutagenesis was applied to complete functional analysis L-Pro throughout life cycle. This indicated L-Pro, addition being proteolysis, also amplification these two are genetically separable. Examination role BYV...

ABSTRACT Epstein-Barr virus nuclear antigen (EBNA) leader protein (EBNALP) is one of the first viral genes expressed upon B-cell infection. EBNALP essential for EBV-mediated immortalization. thought to function primarily by coactivating EBNA2-mediated transcription. Chromatin immune precipitation followed deep sequencing (ChIP-seq) studies highlight that frequently cooccupies DNA sites with host cell transcription factors (TFs), in particular, EP300, implicating a broader role regulation. In...

Abstract Interstitial cystitis/bladder pain syndrome with Hunner's lesion (HIC) is characterized by chronic inflammation and nerve hyperplasia; however, the pathogenesis of HIC remains a mystery. In this study, we detected both Epstein–Barr virus (EBV) latency infection genes EBNA‐1 LMP‐1 EBV lytic BZLF‐1 BRLF‐1 expression in bladders, indicating coexistence persistence reactivation B cells bladders. Upregulation EBV‐associated inflammatory such as TNF‐α IL‐6, suggests implicated bladder...

The Epstein-Barr virus nuclear leader protein LP (EBNALP) and EBNA2 are expressed first in lymphocyte infection, coordinately regulate cell viral gene transcription, critical for outgrowth into lymphoblastoid lines (LCLs). We have now found that EBNALP readily associated with or the C-terminal acidic activation domain (E2AD) when both components were by bacteria. In lymphoblasts, did not stably associate. However, deleted only 10 amino acids lymphoblasts activating from E2AD was essential...

The beet yellows closterovirus leader proteinase (L-Pro) possesses a C-terminal domain and nonproteolytic N-terminal domain. It was found that although L-Pro is not essential for basal-level replication, deletion of its resulted in 1, 000-fold reduction RNA accumulation. Mutagenic analysis the revealed structural flexibility except 54-codon-long, 5'-terminal element corresponding open reading frame critical efficient amplification at both protein levels.

EBNA2 transcriptional activation and regulated EBNALP coactivation are critical for Epstein-Barr virus-infected primary B-lymphocyte growth transformation. requires the acidic domain (E2AD); can bind to E2AD. has now been found less well amino acids 1 58, which identified be a second domain, E2AD2. E2AD2 was specifically coactivated by EBNALP. Moreover, E2AD, E2AD2, RG intermediate between E2AD had significant roles in EBNA2-mediated coactivation.

The binding of Epstein-Barr Virus (EBV) nuclear antigen 1 (EBNA1) to the latent replication origin (oriP) triggers multiple downstream events support virus-induced pathogenesis and tumorigenesis. Although EBV is widely recognized as a B-lymphotropic infectious agent, little known about how tissue-specific factors are involved in establishment latency. Here, we showed that EBNA1 binds B cell activator PAX5 promote EBNA1/oriP-dependent transcription. In addition showing short hairpin RNA...

Abstract In this study the damselfly Ischnura senegalensis (Rambur, 1842) was first found to produce strong photoluminescence (PL) emissions from various colored-body portions, such as eighth abdominal segment of tail. The colors portions can be enhanced or modified by PL for assistance in reducing intrasexual and male harassment, improving mature mating conspecific identity. Therefore, that contribute color modification coloration are involved cuticle evolution damselflies. micro-PL...

Congenital nephrogenic diabetes insipidus (CNDI) is a genetic disorder caused by mutations in arginine vasopressin receptor 2 (AVPR2) or aquaporin genes, rendering collecting duct cells insensitive to the peptide hormone stimulation for water reabsorption. This study reports first identified AVPR2 mutation Taiwan and demonstrates our effort understand pathogenesis applying computational structural analysis tools. The CNDI condition of an 8-month-old male patient was confirmed according...

Originally extracted from Momordica charantia seeds, the antiviral and anti-tumor activities of anti-HIV protein MAP30 have become well known. Although has been reported to possess activity against several human viruses, current understanding MAP30-mediated response is mainly derived previous research work on herbal medicines; mechanistic insight its effects other viruses remains largely unknown. In this study, we showed that both ectopically expressed purified recombinant (rMAP30) impeded...

Background: Based on our recent study findings that Epstein-Barr virus (EBV) may be an etiologic factor for some patients with interstitial cystitis/bladder pain syndrome (IC/BPS), current aimed to investigate urinary viral spectrum in IC/BPS and clinical efficacy of valacyclovir.Methods: Twenty-eight Patients (4 Hunner’s lesion (HIC) 24 without (NHIC)) were prospectively enrolled valacyclovir treatment 500 mg twice a day 4 weeks. Urine samples collected from controls analysis the level...

Originally extracted from Momordica charantia seeds, the antiviral and anti-tumor activities of anti-HIV protein MAP30 have become well known. Although has been reported to possess activity against several human viruses, current understanding MAP30-mediated response is mainly derived previous research work on herbal medicines; mechanistic insight its effects other viruses remains largely unknown. In this study, we showed that both ectopically expressed purified recombinant (rMAP30) impeded...

(Abstract) Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) induces expression of both viral and cellular genes in infected B cells by mimicking activated Notch receptors (Notch-IC) that mediate transcription activation through binding to the repressing domain recombining protein suppressor hairless (RBP-Jκ). In general, chromatin immunoprecipitation (ChIP) assays, electrophoresis mobility shift assays (EMSA), streptavidin-agarose mediated DNA pull-down together with cell-based reporter...