- Essential Oils and Antimicrobial Activity
- Insect Pest Control Strategies
- Sesquiterpenes and Asteraceae Studies
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Entomopathogenic Microorganisms in Pest Control
- Natural product bioactivities and synthesis
- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Plant and fungal interactions
- Allelopathy and phytotoxic interactions
- Phytochemical compounds biological activities
- Insect and Pesticide Research
- Mycorrhizal Fungi and Plant Interactions
- Phytochemistry and Biological Activities
- Plant Toxicity and Pharmacological Properties
- Crystallography and molecular interactions
- Steroid Chemistry and Biochemistry
- Mycotoxins in Agriculture and Food
- Biological Activity of Diterpenoids and Biflavonoids
- Plant tissue culture and regeneration
- Nematode management and characterization studies
- Cancer Treatment and Pharmacology
Instituto de Productos Naturales y Agrobiología
2015-2025
Consejo Superior de Investigaciones Científicas
2013-2024
Hospital Universitario Nuestra Señora de Candelaria
2015-2024
Clinical Trial Investigators
2022
Universidad de La Laguna
1984-2014
Universidad de la República de Uruguay
2012
Central University of Venezuela
2001
Endolichenic fungi represent an important ecological group of microorganisms that form associations with photobionts in the lichen thallus. These endofungi live and coevolve lichens are known for synthesizing secondary metabolites novel structures diverse chemical skeletons making them unexplored microbial community great interest. As part our search new phytoprotectants, this work, we studied endolichenic fungus Xylaria sp. isolated from Hypogymnia tubulosa, which grows as epiphyte on bark...
The new diterpenes brussonol (1) and iguestol (6α,11-dihydroxy-12-methoxy-abieta-8,11,13-triene) (2) with an icetexane a dehydroabietane skeleton, respectively, have been isolated from hairy root cultures of Salvia broussonetii. Other previously known diterpenes, 7-oxodehydroabietane, 11-hydroxy-12-methoxyabietatriene, taxodione, inuroyleanol, ferruginol, deoxocarnosol 12-methyl ether, cryptojaponol, pisiferal, sugiol, isomanool, 14-deoxycoleon U, 6α-hydroxydemethylcryptojaponol,...
The chemical composition of essential oils (EOs) from ten Peruvian Piper species (Piper coruscans, Pc; P. tuberculatum, Pt; casapiense, Pcs; obliquum, Po; dumosum, Pd; anonifolium, Pa; reticulatum, Pr; soledadense, Ps; sancti-felicis, Psf and mituense, Pm) has been studied, along with their antifungal phytotoxic activities. These EOs contained β-bisabolene/nerolidol (Pc), β-bisabolene/δ-cadinene/caryophyllene (Pt), caryophyllene oxide (Pcs), bicyclogermacrene/10-epi-Elemol (Po),...
In the current study, an ethyl acetate extract from endophytic fungus Aspergillus sp. SPH2 isolated stem parts of endemic plant Bethencourtia palmensis was screened for its biocontrol properties against pathogens (Fusarium moniliforme, Alternaria alternata, and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi), parasites (Meloidogyne javanica), ticks (Hyalomma lusitanicum). gave extracts with strong fungicidal ixodicidal effects at different...
Several preparations were obtained from the aerial parts of predomesticated Lavandula luisieri, including essential oil and ethanolic, hexane, ethyl acetate extractives. Additionally, pilot plant vapor pressure extraction was carried out at a range 0.5–1.0 bar to give an aqueous residue. A chemical study hexane extract led isolation six necrodane derivatives (1, 2, 4–7), with four these 5, 7) being new, as well camphor, cadinane sesquiterpene (9), tormentic acid, ursolic acid. The EtOAc EtOH...
Botanical and fungal biopesticides, including endophytes, are in high demand given the current restrictive legislations on use of chemical pesticides. As part an ongoing search for new a series endophytes have been isolated from selected medicinal plants Lauraceae species. In study, extract endophytic fungus Trichoderma sp. EFI 671, stem parts plant Laurus sp., was screened bioactivity against pathogens (Fusarium graminearum, Rhizoctonia solani, Sclerotinia sclerotiorum Botrytis cinerea),...
Given the importance of genus Artemisia as a source valuable natural products, rare plant pedemontana subspecies assoana, endemic to Iberian Peninsula, has been experimentally cultivated in greenhouse and aeroponically, produce biomass for essential oil (EO) extraction. The chemical composition EOs was analyzed, their protection (insects: Spodoptera littoralis, Rhopalosiphum padi, Myzus persicae; plants: Lactuca sativa Lolium perenne; fungi: Aspergillus niger; nematode: Meloidogyne javanica)...
In the current work, production of dioxolanone and meroterpene type secondary metabolites under different fermentation conditions by endophytic fungus Phyllosticta capitalensis (isolate YCC4) isolated from leaves endemic plant Persea indica has been evaluated. Different techniques, such as microparticle-enhanced culture (MPEC: bentonite, talcum powder) surface adhesion (SAF: metallic mesh glass wool) were applied to medium. Ethyl acetate extracts fermentations isolate YCC4 analyzed for their...
The genome of the previously described endophytic fungus Stemphylium sp. (strain Aa22) has been sequenced to near completion. Phylogenomic analysis placed strain Aa22 in close proximity lycopersici . Strain had reported as producer bioactive alkyl-resorcinols stemphol and derivatives stempholones A B solid culture on rice. Genome mining for biosynthetic gene clusters (BGCs) identified 42 genomic regions predicted encode secondary metabolites production. Among them, a single type III...
Abstract The endophytic fungus Aspergillus sp. SPH2 was isolated from the stems of endemic plant Bethencourtia palmensis and its extracts were found to have strong fungicidal effects against Botrytis cinerea ixodicidal Hyalomma lusitanicum at different fermentation times. In this study, grown using three culture media two methodologies, Microparticulate Enhancement Cultivation (MPEC) Semi-Solid-State Fermentation (Semi-SSF), increase production secondary metabolites during submerged...
The search for natural product-based biopesticides from endophytic fungi is an effective tool to find new solutions. In this study, we studied a pre-selected fungal endophyte, isolate YCC4, the paleoendemism Persea indica, along with compounds present in extract and identification of insect antifeedant nematicidal ones. endophyte YCC4 was identified as Phyllosticta sp. by molecular analysis. activity tested choice bioassays against Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi,...
Abstract Botanical biopesticides constitute an important tool for Integrated Pest Management practices. Uruguay has a great potential developing botanical from its abundant native flora. Dodonaea viscosa Jacq. is Sapindaceae that in preliminary studies was shown to possess deterrent activity against insect models. In this work, ethanolic extracts of the leaves species were studied. Bioguided fractionation and supercritical fluid extraction led isolation active compounds. For purpose four...
The continuous search for natural product-based biopesticides from fungi isolated untapped sources is an effective tool. In this study, we studied a pre-selected fungal endophyte, isolate Aa22, the medicinal plant Artemisia absinthium, along with antifungal, insect antifeedant and nematicidal compounds present in extract. endophyte Aa22 was identified as Stemphylium solani by molecular analysis. antifungal activity tested broth microdilution against Fusarium solani, F. oxysporum, moniliforme...