Helicobacter pylori (H. pylori) can evade the host's immune response and persist for a long time on gastric mucosa. T helper (Th) cells appear to be involved in control of H. bacteria but promote mucosal inflammation. In contrast, regulatory (Tregs) may reduce inflammation persistence. CC motif chemokine receptor 6 (CCR6) is migration various into inflamed this study, we examined CCR6

The influenza vaccine Grippol® Quadrivalent (GQ) is a new vaccine, containing the adjuvant Polyoxidonium® and recombinant hemagglutinins from 4 strains of virus in amount 5-6 μg each hemagglutinin per human dose. These doses antigens are about 3 times less than standard dose recommended by WHO. We sought to characterize immune response GQ determine contribution this response. BALB/c mice were vaccinated with or adjuvant-free antigen mixtures (AGs). Then, antibody response, number memory T...

Summary The success of pregnancy depends upon regulatory mechanisms that allow the fetus to survive and develop term in uterus, despite maternal immune cells' awareness paternal alloantigens. At least some these specific are mediated by effect fetal trophoblast cells. In present study we examine human placental cytotrophoblast cells (CTCs) on maturation dendritic (DCs) vitro. For purpose, CTCs were isolated from samples placentae at 5–11 weeks gestation co-cultured with peripheral blood...

We previously found that the number of CCR6 + T-helpers with phenotype effector/effector memory T cells increases in blood patients H. pylori-associated peptic ulcer.The mature and expression chemokine receptor CCR6, which is involved migration lymphocytes to inflamed mucous membrane gastrointestinal tract, suggests these are immune response observed this clinical condition.To better understand pathogenetic role cells, it necessary study their functional activity, specifically, production...

The proteins of many viruses can be assembled into strictly organized structures virus-like particles bearing antigens the original and may also artificially decorated with other pathogens. These contain no viral genome lack infectivity but highly immunogenic therefore being actively used for vaccine development. Undoubtedly, while designing vaccines, it is necessary to take account information about interaction components immune system particularly antigen-presenting cells. This especially...

Dendritic cells (DC) are specialized antigen-presenting cells.One of their function is to deliver antigens from peripheral tissues lymphoid organs by migration controlled chemokines.The aim the investigation was study effect vaccines stimulating cellular or humoral response on expression chemokine receptors CCR7, CXCR4 and CXCR5 DC, assess motility chemokines.Materials Methods.Immature DC derived monocytes in vitro were incubated with vaccines.We used tuberculosis vaccine BCG stimulating,...

Summary The effects of dexamethasone phosphate and interleukin-7 upon the proliferation T-cells production interferon-γ in newborn's cord blood mononuclear cell cultures were studied. capability to enhance T-cell induced by anti-CD3 with some newborn was identified. Dexamethasone suppressed 68-h stimulated both presence without it. However, a cultivation cells dexamethasone, resulted accumulation T-lymphocytes capable producing after restimulation. As result it amount CD7+ concentration...

Introduction. Viral capsid proteins can assemble into virus-like particles lacking infectivity and bearing parental virus antigens or artificially introduced from other pathogens. At least some of such are highly immunogenic could serve as a platform for promising vaccines. In this work, we assessed an effect decorated with SARS-CoV-2 spike protein fragment on human dendritic cell phenotype functional properties. Materials methods. The were assembled using chimeric molecules obtained by...

Introduction. Helicobacter pylori can infect human gastric mucosa and cause various pathological conditions. In the blood of H. pylori-infected patients, level mature CD4+CCR6+ T-lymphocytes, especially pro-inflammatory CCR6+ T-helper types 1 17, significantly increases. Chemokine receptor CCR6 direct cell migration from into inflamed mucosa. this work, we assessed in vitro response circulating CD4+CCR6– T cells against antigens infected intact individuals. Materials methods. Monocytes...

Helicobacter pylori represents a pathogen causing chronic infection in around half of the global human population, which manifestations vary from asymptomatic to developing gastritis and peptic ulcer. The data accumulated suggest that overt clinical types this are associated with lost immunoregulation increased pro-inflammatory cell-mediated immune response triggered by H. pylori-specific T helper cells. Here, we examined degree peripheral blood CD4+ cell maturity related expression...

B-cell receptors can interact with antigen epitopes on various objects: macromolecules, microorganisms or the surface of other cells, e.g., follicular dendritic cells. Accordingly, B one hand, have ability to evaluate location pathogen epitopes, and, they must adapt their receptor apparatus different epitope locations and antigen-bearing properties. Indeed, antibodies better bind objects regular dense arrangement characteristic many pathogens. As a result, such be recognized as...

Талаев В.Ю., Бабайкина О

Клеточная иммунология

Взаимодействие дендритных клеток с микроорганизмами, способными заселять кишечникФедеральное бюджетное учреждение науки «Нижегородский научно-исследовательский институт эпидемиологии и микробиологии им.академика И.Н.Блохиной» Федеральной службы по надзору в сфере защиты прав потребителей благополучия человека, 603950, г.Нижний Новгород, Российская Федерация Резюме Введение.Один из возможных способов доставки вакцинных антигенов слизистые оболочки для индукции мукозального иммунитета...

Dendritic cells are specialized antigen-presenting which required to involve naive T-lymphocytes into immune response. used in numerous research studies, and have also found practical application as a main component of dendritic cell vaccines. In vitro induction their maturation from monocytes using cytokines is the most widely method obtain these cells. present study, we compared phenotypes both immature mature induced adult healthy donors by means recombinant provided different...

Изменение функциональных свойств дендритных клеток при контакте с микроорганизмами родов Bacillus, Lactiplantibacillus и LimosilactobacillusФедеральное бюджетное учреждение науки «Нижегородский научно-исследовательский институт эпидемиологии микробиологии имени академика И.Н.Блохиной» Федеральной службы по надзору в сфере защиты прав потребителей благополучия человека, 603950, г.Нижний Новгород, Российская Федерация Резюме Введение.Ранее для отбора кандидатов на роль бактериальных векторов...

Introduction: Vaccines are one of the most effective means preventing infectious diseases. Their effectiveness and safety guaranteed by studies vaccine properties, during their development mandatory preclinical clinical trials each new vaccine. Additional information on mechanisms action human immune system cells can be obtained using in vitro response models. The objective study was to determine applicability certain methods studying dendritic assessing effect vaccines. Dendritic active...

Талаев В.Ю