A5040012393- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Helicobacter pylori-related gastroenterology studies
- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Immune Cell Function and Interaction
- Erythropoietin and Anemia Treatment
- Galectins and Cancer Biology
- CAR-T cell therapy research
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Viral Infectious Diseases and Gene Expression in Insects
- Erythrocyte Function and Pathophysiology
- Antimicrobial Peptides and Activities
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Cytokine Signaling Pathways and Interactions
- Banking, Crisis Management, COVID-19 Impact
- Transgenic Plants and Applications
- Cellular transport and secretion
- 3D Printing in Biomedical Research
- Autoimmune and Inflammatory Disorders Research
- Protein purification and stability
- Pharmacological Effects of Natural Compounds
Nizhniy Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina
2019-2024
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2020-2022
Genetika
2018-2019
Kurchatov Institute
2018-2019
Voronezh State Medical Academy named after N.N. Burdenko
2016-2018
Privolzhsky Research Medical University
2016
Institute of Bioorganic Chemistry
1999
Helicobacter pylori (H. pylori) can evade the host's immune response and persist for a long time on gastric mucosa. T helper (Th) cells appear to be involved in control of H. bacteria but promote mucosal inflammation. In contrast, regulatory (Tregs) may reduce inflammation persistence. CC motif chemokine receptor 6 (CCR6) is migration various into inflamed this study, we examined CCR6
The influenza vaccine Grippol® Quadrivalent (GQ) is a new vaccine, containing the adjuvant Polyoxidonium® and recombinant hemagglutinins from 4 strains of virus in amount 5-6 μg each hemagglutinin per human dose. These doses antigens are about 3 times less than standard dose recommended by WHO. We sought to characterize immune response GQ determine contribution this response. BALB/c mice were vaccinated with or adjuvant-free antigen mixtures (AGs). Then, antibody response, number memory T...
Recombinant monoclonal antibodies (mAbs) against tumor necrosis factor alpha are widely used in the biopharmaceutical therapy of autoimmune diseases. Currently, a large number drugs based on these available. Accordingly, development products for Russian market is an important goal. The aim current study to describe one such technology. CHO-DG44-derived cell lines producing mAb were developed using two strategies, individual clones and other pools. To obtain recombinant with highly amplified...
Tumor necrosis factor alpha (generally known as cachectin) was noted a hallmark since the time of its discovery and still retains position one most intensely studied molecules in field biomedicine. TNFα is considered prototype molecule family which, on hand, plays an important role regulating normal differentiation, growth metabolism various cells, other acts mediator inflammatory processes human diseases. Intensive interdisciplinary studies biological therapeutic use have led to...
Community-acquired pneumonia remains a most widespread acute infectious disease of socio-economic significance all over the world. Up to 30% patients present with anemia responsible for unfavourable prognosis and elevated mortality. Not infrequently, is not diagnosed during hospital stay und therefore uncorrected. Severe results in enhanced hypercapnia slowed maturation red blood cells bone marrow which facilitates development ischemic syndrome. Hepcidin, mediator inflammation...
We previously found that the number of CCR6 + T-helpers with phenotype effector/effector memory T cells increases in blood patients H. pylori-associated peptic ulcer.The mature and expression chemokine receptor CCR6, which is involved migration lymphocytes to inflamed mucous membrane gastrointestinal tract, suggests these are immune response observed this clinical condition.To better understand pathogenetic role cells, it necessary study their functional activity, specifically, production...
The proteins of many viruses can be assembled into strictly organized structures virus-like particles bearing antigens the original and may also artificially decorated with other pathogens. These contain no viral genome lack infectivity but highly immunogenic therefore being actively used for vaccine development. Undoubtedly, while designing vaccines, it is necessary to take account information about interaction components immune system particularly antigen-presenting cells. This especially...
Dendritic cells (DC) are specialized antigen-presenting cells.One of their function is to deliver antigens from peripheral tissues lymphoid organs by migration controlled chemokines.The aim the investigation was study effect vaccines stimulating cellular or humoral response on expression chemokine receptors CCR7, CXCR4 and CXCR5 DC, assess motility chemokines.Materials Methods.Immature DC derived monocytes in vitro were incubated with vaccines.We used tuberculosis vaccine BCG stimulating,...
Anemia of chronic disease (ACD) is one the most frequent forms anemia often observed in patients with infections, cancer and inflammatory or autoimmune diseases. The underlying mechanisms are complex include dysregulation iron homeostasis erythropoietin production, impaired proliferation erythroid progenitor cells reduced life span red blood cells. Moreover, ACD superimposed by malnutrition, bleeding renal failure. mediated through cytokines characterized low serum (hypoferremia) increased...
Introduction. Viral capsid proteins can assemble into virus-like particles lacking infectivity and bearing parental virus antigens or artificially introduced from other pathogens. At least some of such are highly immunogenic could serve as a platform for promising vaccines. In this work, we assessed an effect decorated with SARS-CoV-2 spike protein fragment on human dendritic cell phenotype functional properties. Materials methods. The were assembled using chimeric molecules obtained by...
Introduction. Helicobacter pylori can infect human gastric mucosa and cause various pathological conditions. In the blood of H. pylori-infected patients, level mature CD4+CCR6+ T-lymphocytes, especially pro-inflammatory CCR6+ T-helper types 1 17, significantly increases. Chemokine receptor CCR6 direct cell migration from into inflamed mucosa. this work, we assessed in vitro response circulating CD4+CCR6– T cells against antigens infected intact individuals. Materials methods. Monocytes...
Within the presented study, a combination of N-acetyl-D-mannosamine, sodium butyrate, and galactose additives was showed for use during cultivation CHO cells producing darbepoetin alfa. The addition these chemicals enabled an increase in yield highly sialylated forms
Helicobacter pylori represents a pathogen causing chronic infection in around half of the global human population, which manifestations vary from asymptomatic to developing gastritis and peptic ulcer. The data accumulated suggest that overt clinical types this are associated with lost immunoregulation increased pro-inflammatory cell-mediated immune response triggered by H. pylori-specific T helper cells. Here, we examined degree peripheral blood CD4+ cell maturity related expression...
B-cell receptors can interact with antigen epitopes on various objects: macromolecules, microorganisms or the surface of other cells, e.g., follicular dendritic cells. Accordingly, B one hand, have ability to evaluate location pathogen epitopes, and, they must adapt their receptor apparatus different epitope locations and antigen-bearing properties. Indeed, antibodies better bind objects regular dense arrangement characteristic many pathogens. As a result, such be recognized as...
Supported by research grants from the Russian Human Genome Program and TheNorwegian Cancer Society.Received 13 November 1998; manuscript accepted 2 December 1998.Request reprints Dr. Alexander Graphodatsky, Institute of Cytology andGenetics, RAS, Siberian Branch, Novosibirsk, 630090 (Russia);telephone: +007 3832 333857; fax: 007 331278;e-mail: graf@bionet.nsc.ru
Цель обзора - представление новых, патогенетически обоснованных подходов к лечению анемии хронических заболеваний. Анемия заболеваний часто сопутствует многим хроническим инфекциям (инфекционный эндокардит, остеомиелит, туберкулез), злокачественным новообразованиям, ревматическим болезням (ревматоидный артрит, системная красная волчанка, васкулиты), хронической почечной недостаточности. Генез такой довольно сложен, многообразен и недостаточно изучен. Гепсидин является важнейшим пептидом,...
Клеточная иммунология
Взаимодействие дендритных клеток с микроорганизмами, способными заселять кишечникФедеральное бюджетное учреждение науки «Нижегородский научно-исследовательский институт эпидемиологии и микробиологии им.академика И.Н.Блохиной» Федеральной службы по надзору в сфере защиты прав потребителей благополучия человека, 603950, г.Нижний Новгород, Российская Федерация Резюме Введение.Один из возможных способов доставки вакцинных антигенов слизистые оболочки для индукции мукозального иммунитета...
В статье представлены этапы развития бюджетно-налоговых отношений в современной России, предложено считать 2020 год началом нового этапа ввиду неблагоприятной конъюнктуры на мировых рынках нефти, валютном рынке, условиях пандемии коронавируса. Рассмотрены реализованные мероприятия по развитию налогово-бюджетных до года. Представлен обзор фискальных и иных мер поддержки государств с наиболее развитой экономикой. Сделан вывод о трансформации отношений, обусловленной мерами противодействия...
A strategy for optimization of the glycosylation profile Adalimumab, a monoclonal antibody to TNF6 produced by CH O culture has been described. Adalimumab is an analog that serves as substance Humira preparation, product Abbot GmbH & Co. KG (USA). Culturing in closed volume with periodical feed addition (fed-batch regime) was basis developed technology. great number media and feeds from various manufacturers were tested. All them contained no components animal origin supported CHO cell line...