A5070825468- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Organ Donation and Transplantation
- Transplantation: Methods and Outcomes
- Renal Diseases and Glomerulopathies
- Extracellular vesicles in disease
- Pharmacological Effects and Toxicity Studies
- Renal and related cancers
- Mesenchymal stem cell research
- Renal and Vascular Pathologies
- Organ and Tissue Transplantation Research
- Pregnancy and Medication Impact
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Renal cell carcinoma treatment
- Mycobacterium research and diagnosis
- Tissue Engineering and Regenerative Medicine
- Neurological Complications and Syndromes
- Liver Disease and Transplantation
- Immune Cell Function and Interaction
- Polyomavirus and related diseases
- Neurological Disease Mechanisms and Treatments
- Chemokine receptors and signaling
- Genetic and Kidney Cyst Diseases
- T-cell and B-cell Immunology
Sun Yat-sen University
2016-2025
The First Affiliated Hospital, Sun Yat-sen University
2016-2025
Key Laboratory of Guangdong Province
2019-2024
Guangdong Food and Drug Vocational College
2017
Guangzhou Medical University
2017
Centre Hospitalier de l’Université de Montréal
2007
Université de Montréal
2007
Hôpital Notre-Dame
2007
Rationale: Ischemia/reperfusion injury (IRI) is a major cause of acute kidney (AKI) that associated with high morbidity and mortality, for which specific treatments are lacking. In this study, we investigated the protective effect human urine-derived stem cells (USCs) their exosomes against IRI-induced AKI to explore potential these as new therapeutic strategy. Methods: USCs were derived from fresh urine. Cell surface marker expression was analyzed by flow cytometry determine characteristics...
The deleterious side effects of calcineurin inhibitors have impaired long-term survival after renal allograft. New immunotherapy regimens that minimize or even eliminate are required to improve transplantation outcome. Mesenchymal stem cells (MSCs) represent a unique cell population with immunosuppressive function and prolong allograft in experimental organ transplant models.In this pilot study, donor-derived bone marrow MSCs combined sparing dose tacrolimus (50% standard dose) were...
// Guang-hui Pan 1,* , Zheng Chen Lu Xu 1 Jing-hui Zhu Peng Xiang 2 Jun-jie Ma Yan-wen Li Xiao-yong Jia-li Fang Yu-he Guo Lei Zhang and Long-shan Liu 3 The Transplantation Centre, Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China Center for Stem Cell Biology Tissue Engineering, SunYat-sen Guangdong, Laboratory of General Surgery, First Sun Yat-sen * These authors contributed to the work equally should be regarded as co-first Correspondence to: Pan, email: Keywords :...
Rationale: Mesenchymal stem cells (MSCs) have been demonstrated to ameliorate inflammatory bowel disease by their actions on multiple immune cells, especially regulatory B (Breg cells). However, the phenotypes and functions of human MSCs (hMSCs)-treated Breg cell subsets are not yet clear. Methods: Purified were cocultured with immunomodulatory analyzed FACS proliferation assays in vitro. Also, a trinitrobenzenesulfonic acid-induced mouse colitis model was employed detect function...
Objectives: To evaluate the diagnostic performance of donor-derived plasma cell-free DNA (cfDNA) in discriminating antibody-mediated rejection or de novo donor-specific antibodies (DSA) without histological lesions kidney allograft recipients. Methods: In this prospective single center observational study, we enrolled recipients between November, 2016 and September, 2017 at First Affiliated Hospital Sun Yat-sen University. Kidney with (ABMR), DSA but no negative stable renal function were...
There is an urgent need for developing new immunosuppressive agents due to the toxicity of long-term use broad after organ transplantation. Comprehensive sample analysis revealed dysregulation FGL1/LAG-3 and PD-L1/PD-1 immune checkpoints in allogeneic heart transplantation mice clinical kidney transplant patients. In order enhance these two signal axes, a bioengineering strategy developed simultaneously display FGL1/PD-L1 (FP) on surface small extracellular vesicles (sEVs). Among various...
Comprehension of the anatomical characteristics pediatric kidney tumors is crucial for making surgical decisions. Previous tumor nephrometry systems failed to incorporate two significant factors: thrombus and multifocality. We develop a refined system based on comprehensive understanding exhibited by tumors. The TUMORS scoring comprises 6 indicators, including (T)hrombus, (U)rinary collecting involvement, (M)ultiple tumors, (O)utward property, (R)adius, (S)ite relative polar lines. Each...
ABSTRACT Organ transplantation is the most effective treatment for end‐stage organ disease. One of major challenges in shortage. For this reason, more and extended criteria donor organs, including those from donation after circulatory determination death (DCDD), are used clinical practice. However, DCDD organs suffer additional warm ischemic damage, which seriously affects transplant outcomes utilization. Recent studies at home abroad have shown that application normothermic regional...
Aims: To evaluate the influences of CYP3A4, CYP3A5, MDR1 and NR1I2 polymorphisms on tacrolimus concentration in early postrenal transplant recipients. Patients & methods: A total 159 patients were included, dose-adjusted trough day 7 after transplantation (C0D7/D) was calculated 10 SNPs four genes genotyped. Results: CYP3A5*3 explained 32.8% variability C0D7/D. CYP3A4*1G, 1236–2677–3435 diplotype -25385C > T 21.4% C0D7/D CYP3A5 nonexpressers. Conclusion: predominant determinant affecting...
Tacrolimus is a potent but expensive first-line immunosuppressant, thus solutions to reduce tacrolimus consumption while maintain therapeutic level are in urgent need. A two-phase prospective study was conducted assess the efficacy of an ethanolic extraction preparation <i>Schisandra sphenanthera</i> (Wuzhi tablet) as tacrolimus-sparing agent renal transplant recipients who were high-dose consumers (<i>CYP3A5*1</i> allele carriers, CYP3A5 expressers). total 12 patients included Part I study....
Background Dual kidney transplantation (DKT) from small pediatric donors, either en-bloc or split dual transplantation, contributes to mitigating organ scarcity. This study investigates the prognosis of DKT deceased and influencing factors.
Introduction Anemia is prevalent after kidney transplantation (KTx) and associated with reduced graft survival. The associations between temporal changes in hemoglobin (Hb) level the early posttransplant period left ventricular (LV) atrial (LA) function strain are unknown. Methods study cohort included 71 successful pediatric KTx recipients January 2021 September 2022. Echocardiography was used to evaluate cardiac structure, function, both before KTx. Temporal Hb values within 6 months were...
Abstract A liquid chromatography/tandem mass spectrometry (LC‐MS/MS) method was developed and validated for determining tacrolimus (FK506) in rat tissues to study the effect of Schisandra sphenanthera extract on FK506 tissue distribution. After a liquid–liquid extraction with ethyl acetate, ascomycin (IS) were subjected LC‐MS/MS analysis using positive electrospray ionization under multiple reactions monitoring mode. Chromatographic separation achieved Hypersil BDS C 18 column mobile phase...
Ligustrazine (LIG) is a purified and chemically identified component of the Chinese herb Ligusticum wallichii Franchat. It potent blocker vasoconstriction has strong scavenger oxygen free radicals. We investigated effect LIG on renal tubulointerstitial fibrosis using rat model unilateral ureteral obstruction. treatment significantly reduced scores interstitial collagen deposition, amounts hydroxyproline, density myofibroblasts macrophages, transforming growth factor-β1 (TGF-β1) connective...
Although mesenchymal stromal cells (MSCs) possess immunomodulatory properties and exhibit promising efficacy against chronic graft-versus-host disease (cGVHD), little is known about the immune changes by which MSCs ameliorate cGVHD in vivo. Recent studies have suggested that B lymphocytes might play an important role pathogenesis of cGVHD. In this study, we investigated numbers, phenotypes, subpopulations patients who showed a complete response (CR), partial (PR), or no (NR) after MSC...
Golgi phosphoprotein 3 (GOLPH3) has been identified as an oncoprotein in various human cancers; however, its role pancreatic ductal adenocarcinoma (PDAC) is unknown. We examined GOLPH3 expression levels and relationship with survival patients PDAC to establish the significance of development progression PDAC. Real-time qPCR Western blotting were performed analyze mRNA protein paired tumor adjacent non-tumor tissues. Immunohistochemistry was used paraffin-embedded tissues from 109 cases...
Early graft loss and poor function limit the use of kidneys from infant donors. Six en bloc kidney transplantations were performed donors younger than 10 months into pediatric recipients between November 2012 September 2015 at our center. We retrospectively analyzed recipient donor demographics, surgery procedures, complications, size, patient survival with a follow-up 6-39 (median 15.5 months). Donor age ranged 1 to weight ranging 3.5 kg. Recipient 16 years 30 39 One was removed due...