In addition to frequent epidemics of group A meningococcal disease, endemic bacterial meningitis due mostly Neisseria meningitidis, pneumococcus, and Haemophilus influenzae type b is a serious problem in sub-Saharan Africa. The improved ability identify the etiologic agent cases will facilitate more rapid administration precise therapy.To describe epidemiology evaluate usefulness field-based polymerase chain reaction (PCR) testing, we implemented population-based surveillance Burkina Faso...

Public health and clinical strategies for meningitis epidemics in sub-Saharan Africa usually assume that Neisseria meningitidis infection causes most disease.During 24 months from 2002 to 2005, we collected laboratory information suspected acute bacterial cases 3 districts Burkina Faso. Streptococcus pneumoniae was identified by culture, polymerase chain reaction, or antigen detection cerebrospinal fluid. Pneumococcal genotyping performed on strains using multilocus variable-number tandem...

During 1994 and 1995, the prevalence of hepatitis C virus (HCV) its genotypes were studied in several rural urban populations three West African countries: Guinea, Burkina Faso, Benin. The following groups screened for antibodies to HCV (anti-HCV): 459 villagers forest region Guinea; 965 individuals urban, suburban, Bobo Dioulasso area, Faso; 582 blood donors Cotonou, In Benin, 60 patients with sickle cell anemia (30 30 without history multiple transfusion) 13 hospital liver disease also...

During 1994 and 1995, the prevalence of hepatitis C virus (HCV) its genotypes were studied in several rural urban populations three West African countries: Guinea, Burkina Faso, Benin. The following groups screened for antibodies to HCV (anti-HCV): 459 villagers forest region Guinea; 965 individuals urban, suburban, Bobo Dioulasso area, Faso; 582 blood donors Cotonou, In Benin, 60 patients with sickle cell anemia (30 30 without history multiple transfusion) 13 hospital liver disease also...

Streptococcus pneumoniae causes a substantial proportion of meningitis cases in the African belt; however, few reports exist to quantify its burden and characteristics. We conducted population-based sentinel hospital surveillance acute bacterial among persons all ages Burkina Faso Togo 2002–2006. S. other organisms were identified by culture, polymerase chain reaction, or detection antigen cerebrospinal fluid (CSF). Information was collected on 2843 patients with suspected meningitis. CSF...

Abstract The study assessed changes in nutritional content of some commonly consumed traditional vegetables subjected to postharvest processes. Amaranth ( Amaranthus cruentus L.), black nightshade Solanum scabrum Mill.) and jute mallow Corchorus olitorius L.) leaves used as were blanching, boiling drying. proximate composition β‐carotene fresh processed determined. Amaranth, had 25.21%, 39.74% 29.18% protein, respectively. levels 16.40, 25.25 27.74 mg/100 g for amaranth leaves, ash was...

Background. During the period 2001–2002, Burkina Faso reported its first meningitis epidemic due to Neisseria meningitidis (Nm) serogroup W135, prompting concerns that this would persist as a cause of disease.

During reinforced surveillance of acute bacterial meningitis in Burkina Faso, meningococcal strains phenotype NG:NT:NST were isolated from cerebrospinal fluid samples 3 patients. The negative for the ctrA gene but positive crgA gene. Molecular typing revealed that harbored capsule null locus (cnl) and belonged to multilocus sequence type (ST)-192. PorA sequencing showed all either P1.18-11,42; P1.18,42-1; P1.18-11,42-1; P1.18-11,42-3; or P1.18-12,42-1. Sequencing also FetA receptor Serum...

Abstract Cassava ( Manihot esculenta Crantz) is a food plant introduced in Africa from America by the Portuguese 1558. The objective of this study to establish cassava origins, production, and utilization Burkina Faso. investigation was carried out regions Center West, Cascades, Boucle du Mouhoun, Hauts Bassins, South East Eighteen processing units 226 farmers 57 communities selected have been involved survey. showed that Faso, former Upper Volta costal countries, Gold Coast (now Ghana),...

High-risk human papillomavirus (HPV) is found in over 99% of cervical cancers. The aim this study was to determine the prevalence HPV a population women Bobo-Dioulasso and identify high-risk types present these women. From May June, 2015, 181 who came for consultation at Souro Sanou University Hospital have been included study. Uterine endocervical swabs taken DNA obtained by extraction from samples thus collected used genotypes through real-time PCR. age ranged 20 56 years with mean...

Spontaneous fermentation or traditional method of preserving indigenous foods using several microorganisms, is frequently practiced in the marginal world. Fermented seeds and milk are mostly consumed form food condiments desserts Africa, Asia others parts Our previous studies deal with production Bacteriocin-Like Inhibitory Substances by Bacillus from fermented quality Burkina Faso. The rep-PCR sequencing were used to characterize thirty eights strains isolated various Phylogenetic tree...

The determination of CD4 cells is crucial clinical importance for patients with AIDS. However, the high costs involved represent limitations cell counting in developing countries. In order to provide an affordable technique, we introduced a simplified volumetric (SVC) technique without sample manipulations and investigated it multicentre study. Blood samples from 434 healthy donors immunodeficient were tested eight hospital laboratories Europe, Africa Asia. counts compared using in-house...

To evaluate risk factors for meningococcal carriage and acquisition in the African meningitis belt, comparing epidemic serogroup A (NmA) to non-epidemic serogroups.During season of 2003, pharyngeal swabs were taken at five monthly visits a representative population sample (N = 488) Bobo-Dioulasso, Burkina Faso (age 4-29 years) analysed by culture. Standardized questionnaires administered. In 2006, similar study was performed 624 individuals 1-39 during an NmA epidemic. We evaluated...

The emergence and spread of carbapenem resistance in Gram-negative bacilli such as Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa through the production carbapenemases is a global phenomenon. It threatens patient care leads to therapeutic impasses. This study aims genotypically determine prevalence most frequent carbapenemase genes among multidrug-resistant E. coli strains isolated from patients at biomedical analysis laboratory. A total fifty-three...

Artemisinin-based combinations therapy (ACT) is the current frontline curative for uncomplicated malaria in Burkina Faso. Sulfadoxine-pyrimethamine (SP) used preventive treatment of pregnant women (IPTp), while SP plus amodiaquine (SP-AQ) recommended children under five seasonal chemoprevention (SMC). This study aimed to assess proportions mutations P. falciparum multidrug-resistance 1 (Pfmdr1), chloroquine resistance transporter (Pfcrt), dihydrofolate reductase (pfdhfr), and dihydropteroate...

The genetic control of blood infection levels in human malaria remains unclear. Case studies have not demonstrated a strong association between candidate genes and parasite densities as opposed to surveys that focused on severe malaria. As an alternative approach, we used segregation analyses determine the parasitemia. We surveyed 509 residents (53 pedigrees) rural area 389 (41 urban during 18 months. Each family was visited 20 times 28 area; mean number parasitemia measurements per subject...

We sought to describe Neisseria meningitidis immunity and its association with pharyngeal carriage in Burkina Faso, where N. serogroup W-135 A disease are hyperendemic most of the population received polysaccharide A/C vaccine during 2002.We collected oropharyngeal swab samples from healthy residents Bobo-Dioulasso (4-14 years old, n=238; 15-29 n=250) monthly February-June 2003; isolates were analyzed using polymerase chain reaction serogrouped immune sera. Serum at first last clinic visit...

To describe Neisseria meningitidis strains in the African meningitis belt 2003, we obtained 2,389 oropharyngeal swabs at 5 monthly visits a representative population sample (age range 4-29 years) Bobo-Dioulasso, Burkina Faso. A total of 152 carriage isolates were grouped, serotyped, and genotyped. Most NG:NT:NST sequence type (ST) 192 (63% all N. meningitidis), followed by W135:2a:P1.5,2 ST-11 (16%) NG:15:P1.6 ST-198 (12%). We also found ST-2881 (W135:NT:P1.5,2), ST-751 (X:NT:P1.5), ST-4375...

Background. To better understand localized meningococcal meningitis epidemics, we evaluated a serogroup A (NmA) epidemic in Burkina Faso by surveillance, carriage, and seroprevalence studies. Methods. During March–April 2006, cerebrospinal fluid samples from patients suspected to have 3 villages were analyzed culture or polymerase chain reaction. We assessed carriage serogroup-specific serum bactericidal antibody titers with baby rabbit complement (rSBA) representative population sample (N =...

Abstract In Burkina Faso, flamed/grilled chickens are very popular and well known to consumers. The aim of this study was evaluate the microbiological quality, antibiotic resistance, virulence gene from Escherichia coli isolated these in Ouagadougou. A total 102 grilled, flamed, fumed were collected Ouagadougou analyzed, using standard methods. All E. isolates checked with antimicrobial test also typed by 16‐plex PCR . mean aerobic mesophilic bacteria ( AMB ) thermo‐tolerant coliforms TTC...

Swartzia madagascariensis, Combretum glutinosum and Tinospora bakis are three plants of the folk medicine used by healers in Burkina Faso for treatment malaria. A scientific validation this utilization was not previously made. Aqueous, methanol, hydromethanol extracts from roots bark S. methanol leaves C. aqueous alkaloidal T. were also made their antimalarial activity screened against Plasmodium falciparum chloroquine-resistant strain W2 vitro. The screening showed that active (5 µg/ml <...

Objectif : La présente étude vise à évaluer les pratiques phytosanitaires des maraîchers et fournir une meilleure connaissance risques liés aux de maraîchage, notamment l'utilisation pesticides intrants chimiques Ouagadougou au Burkina Faso.Méthodologie résultats Des enquêtes ont été réalisées du 10 mars 08 Avril 18 Juillet 06 Août 2016 auprès 200 producteurs dans sites Tanghin Boulmiougou où se pratique forte activité production maraîchère. Les observations révélées utilisation non...

Many men who have sex with (MSM) are at significant risk for HIV infection. The objective of this study was to determine the prevalence and correlates infection among MSM in Burkina Faso. A cross-sectional biological behavioral survey conducted from January August 2013 Ouagadougou Bobo-Dioulasso. 18 years old above were recruited using respondent driven sampling (RDS). administered participants followed by testing. Population estimates 95% confidence intervals (CI) adjusted RDS design...