A5101776165- Hemoglobinopathies and Related Disorders
- Molecular spectroscopy and chirality
- NMR spectroscopy and applications
- Chronic Lymphocytic Leukemia Research
- Multiple Myeloma Research and Treatments
- Advanced NMR Techniques and Applications
- Electron Spin Resonance Studies
- DNA Repair Mechanisms
- Protein Degradation and Inhibitors
- Iron Metabolism and Disorders
- Histone Deacetylase Inhibitors Research
- Spectroscopy and Quantum Chemical Studies
- Metal complexes synthesis and properties
- Pharmacological Effects and Toxicity Studies
- Acute Myeloid Leukemia Research
- DNA and Nucleic Acid Chemistry
- Cannabis and Cannabinoid Research
- Lung Cancer Treatments and Mutations
- CRISPR and Genetic Engineering
- Cell Adhesion Molecules Research
- Fluorine in Organic Chemistry
- Lanthanide and Transition Metal Complexes
- HIV/AIDS drug development and treatment
- Advanced MRI Techniques and Applications
- Enzyme Structure and Function
Yale University
2012-2023
Seattle University
2023
Neurological Surgery
2023
Virginia Mason Medical Center
2023
University of Utah
2023
Penn State Milton S. Hershey Medical Center
2023
Creative Commons
2023
University of Pennsylvania Health System
2023
University of California, San Francisco
1990-2022
CSL (United States)
2022
A study was conducted to determine the fidelity of DNA synthesis catalyzed in vitro by reverse transcriptase from a human immunodeficiency virus type 1 (HIV-1). Like other retroviral transcriptases, HIV-1 enzyme does not correct errors exonucleolytic proofreading. Measurements with M13mp2-based assays indicated that enzyme, isolated either particles or Escherichia coli cells infected plasmid expressing cloned gene, exceptionally inaccurate, having an average error rate per detectable...
Background: Researchers of sickle cell disease have traditionally used health care utilization as a proxy for pain and underlying vaso-occlusion. However, may not completely reflect the amount self-reported or acute, painful episodes (crises). Objective: To examine prevalence relationship among pain, crises, in adults with disease. Design: Prospective cohort study. Setting: Academic community practices Virginia. Patients: 232 patients age 16 years older Measurements: Patients completed daily...
A general repair process for DNA heteroduplexes has been detected in HeLa cell extracts. Using a variety of M13mp2 substrates containing single-base mismatches and extra nucleotides, extensive is observed after incubation with cytoplasmic extracts subsequent transfection bacterial cells the treated DNA. Most, but not all, mispairs as well two frameshift are repaired efficiently. Parallel measurements Escherichia coli suggest that specificities similar systems. The presence nick molecule...
<h3>Importance</h3> Zoledronic acid, a third-generation aminobisphosphonate, reduces the incidence of skeletal-related events and pain in patients with bone metastases. The optimal dosing interval for zoledronic acid is uncertain. <h3>Objective</h3> To determine whether administered every 12 weeks noninferior to 4 weeks. <h3>Design, Setting, Participants</h3> Randomized, open-label clinical trial conducted at 269 academic community sites United States. Patients (n = 1822) metastatic breast...
DNA-dependent DNA synthesis in vitro by human immunodeficiency virus-1 reverse transcriptase is relatively error-prone. The enzyme, whether recombinant or from virus particles, produces errors while replicating M13mp2 at a rate that, if operative vivo, would produce about five mutations per genome round of replication. Sequence analysis mutants resulting demonstrates that are nonrandomly distributed. Base substitution and one-base frameshift mutational hot spots observed. specificity...
Abstract Background Sickle cell disease (SCD) is a chronic associated with high degrees of morbidity and increased mortality. Health-related quality life (HRQOL) among adults sickle has not been widely reported. Methods We administered the Medical Outcomes Study 36-item Short-Form to 308 patients in Pain Cell Epidemiology (PiSCES) assess HRQOL. Scales included physical function, emotional role bodily pain, vitality, social mental health, general health. compared scores national norms using...
Depression and anxiety are common in sickle cell disease (SCD) but relatively little is known about their impact on SCD adults. This study measured prevalence of depression adults, effects crisis noncrisis pain, quality-of-life, opioid usage, healthcare utilization.The Pain Sickle Cell Epidemiology Study a prospective cohort 308 Baseline variables included demographics, genotype, laboratory data, health-related depression, anxiety. Subjects completed daily diaries for up to 6 months,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNitrogen-15 nuclear magnetic resonance spectroscopy. The state of histidine in the catalytic triad .alpha.-lytic protease. Implications for charge-relay mechanism peptide-bond cleavage by serine proteasesWilliam W. Bachovchin and John D. RobertsCite this: J. Am. Chem. Soc. 1978, 100, 26, 8041–8047Publication Date (Print):December 1, 1978Publication History Published online1 May 2002Published inissue 1 December...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNitrogen-15 Magnetic Resonance Spectroscopy. II. Coupling ConstantsGerhard. Binsch, Joseph B. Lambert, Bryan W. Roberts, and John D. RobertsCite this: J. Am. Chem. Soc. 1964, 86, 24, 5564–5570Publication Date (Print):December 1, 1964Publication History Published online1 May 2002Published inissue 1 December 1964https://pubs.acs.org/doi/10.1021/ja01078a033https://doi.org/10.1021/ja01078a033research-articleACS PublicationsRequest reuse...
We determined the fidelity of avian myeloblastosis virus and Moloney murine leukemia reverse transcriptases (RTs) during DNA synthesis in vitro using M13mp2 lacZ alpha gene as a mutational target. Both RTs commit an error approximately once for every 30,000 nucleotides polymerized. sequence analysis mutants generated forward mutation assay capable detecting many types errors demonstrated that RT produced variety different mutations. The majority (58%) were single-base substitutions; all...
The O protein of bacteriophage lambda localizes the initiation DNA replication to a unique site on genome, ori lambda. By means electron microscopy, we infer that binding initiates series addition and transfer reactions culminate in localized unwinding origin DNA, generating prepriming structure for replication. We can define three stages this reaction, first two which have characterized previously. First, dimeric binds multiple sites self-associates form nucleoprotein structure, O-some....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNitrogen-15 nuclear magnetic resonance spectroscopy of some nucleosides and nucleotidesVolker Markowski, Glenn R. Sullivan, John D. RobertsCite this: J. Am. Chem. Soc. 1977, 99, 3, 714–718Publication Date (Print):February 1, 1977Publication History Published online1 May 2002Published inissue 1 February 1977https://pubs.acs.org/doi/10.1021/ja00445a009https://doi.org/10.1021/ja00445a009research-articleACS PublicationsRequest reuse permissionsArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear magnetic resonance spectroscopy. Benzene-13CFrank J. Weigert and John D. RobertsCite this: Am. Chem. Soc. 1967, 89, 12, 2967–2969Publication Date (Print):June 1, 1967Publication History Published online1 May 2002Published inissue 1 June 1967https://pubs.acs.org/doi/10.1021/ja00988a029https://doi.org/10.1021/ja00988a029research-articleACS PublicationsRequest reuse permissionsArticle Views409Altmetric-Citations138LEARN ABOUT THESE...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear magnetic resonance spectroscopy. Carbon-13 spectra of aliphatic carboxylic acids and carboxylate anionsR. Hagen John D. RobertsCite this: J. Am. Chem. Soc. 1969, 91, 16, 4504–4506Publication Date (Print):July 1, 1969Publication History Published online1 May 2002Published inissue 1 July 1969https://pubs.acs.org/doi/10.1021/ja01044a032https://doi.org/10.1021/ja01044a032research-articleACS PublicationsRequest reuse permissionsArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear magnetic resonance spectroscopy. Carbon-13 chemical shifts of small peptides as a function pHManfred Christl and John D. RobertsCite this: J. Am. Chem. Soc. 1972, 94, 13, 4565–4573Publication Date (Print):June 1, 1972Publication History Published online1 May 2002Published inissue 1 June 1972https://pubs.acs.org/doi/10.1021/ja00768a026https://doi.org/10.1021/ja00768a026research-articleACS PublicationsRequest reuse permissionsArticle...
// Hatem H. Soliman 1 , Susan E. Minton Hyo Sook Han Roohi Ismail-Khan Anthony Neuger Fatema Khambati David Noyes Richard Lush Alberto A. Chiappori John D. Roberts 2 Charles Link 3 Nicholas N. Vahanian Mario Mautino Howard Streicher 4 Daniel M. Sullivan and Scott J. Antonia Lee Moffitt Cancer Center Research Institute, Tampa, Florida, USA Massey Center, Virginia Commonwealth University, Richmond, Virginia, NewLink Genetics Inc., Ames, Iowa, Therapeutics Evaluation Program, National Bethesda,...
The gut microbiome, a key constituent of the colonic environment, has been implicated as an important modulator human health. eukaryotic epigenome is postulated to respond environmental stimuli through alterations in chromatin features and, ultimately, gene expression. How host mediates epigenomic responses microbiota emerging area interest. Here, we profile microbiome and characteristics colon epithelium from mice fed either obesogenic or control diet, followed by analysis resultant changes...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear magnetic resonance spectroscopy. Carbon-13 spectra of some inositols and their O-methylated derivativesDouglas E. Dorman, S. J. Angyal, John D. RobertsCite this: Am. Chem. Soc. 1970, 92, 5, 1351–1354Publication Date (Print):March 1, 1970Publication History Published online1 May 2002Published inissue 1 March 1970https://pubs.acs.org/doi/10.1021/ja00708a040https://doi.org/10.1021/ja00708a040research-articleACS PublicationsRequest reuse...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrientation in Aminations of Substituted Halobenzenes1John D. Roberts, C. Wheaton Vaughan, L. A. Carlsmith, and Dorothy SemenowCite this: J. Am. Chem. Soc. 1956, 78, 3, 611–614Publication Date (Print):February 1, 1956Publication History Published online1 May 2002Published inissue 1 February 1956https://doi.org/10.1021/ja01584a025RIGHTS & PERMISSIONSArticle Views593Altmetric-Citations88LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant...
Abstract GATA3 is frequently mutated in breast cancer; these mutations are widely presumed to be loss-of function despite a dearth of information regarding their effect on disease course or mechanistic impact the cancer transcriptional network. Here, we address molecular and clinical features associated with mutations. A novel classification scheme defines distinct for patients bearing tumors second zinc-finger (ZnFn2). An engineered ZnFn2 mutant cell line by CRISPR–Cas9 reveals that...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSmall-Ring Compounds. III. Synthesis of Cyclobutanone, Cyclobutanol, Cyclobutene and CyclobutaneJohn D. Roberts Charles W. SauerCite this: J. Am. Chem. Soc. 1949, 71, 12, 3925–3929Publication Date (Print):December 1, 1949Publication History Published online1 May 2002Published inissue 1 December 1949https://pubs.acs.org/doi/10.1021/ja01180a013https://doi.org/10.1021/ja01180a013research-articleACS PublicationsRequest reuse permissionsArticle...