A5066276029- Circadian rhythm and melatonin
- Light effects on plants
- Plant Molecular Biology Research
- Photoreceptor and optogenetics research
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- Spaceflight effects on biology
- Cancer therapeutics and mechanisms
- Pharmacogenetics and Drug Metabolism
- Adipose Tissue and Metabolism
- Neurobiology and Insect Physiology Research
- Neurogenesis and neuroplasticity mechanisms
- Plant and fungal interactions
Kocaeli Üniversitesi
2024
Stem Cell Institute
2024
Koç University
2020-2023
Despite strict measures taken by many countries, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to be an issue of global concern. Currently, there are no clinically proven pharmacotherapies for disease 2019, despite promising initial results obtained from drugs such as azithromycin and hydroxychloroquine. Therefore, the repurposing approved use against SARS-CoV-2 has become a viable strategy. Here, we searched that target 3C-like protease (3CLpro) viral RNA-dependent...
Abstract Cryptochromes are negative transcriptional regulators of the circadian clock in mammals. It is not clear how reducing level endogenous CRY1 mammals will affect rhythm and relation such a decrease with apoptosis. Here, we discovered molecule (M47) that destabilizes Cryptochrome 1 (CRY1) both vitro vivo. The M47 selectively enhanced degradation rate by increasing its ubiquitination resulted period length U2OS Bmal1 -d Luc cells. In addition, subcellular fractionation studies from mice...
Mammalian circadian clocks are driven by transcription/translation feedback loops composed of positive transcriptional activators (BMAL1 and CLOCK) negative repressors (CRYPTOCHROMEs (CRYs) PERIODs (PERs)). CRYs, in complex with PERs, bind to the BMAL1/CLOCK repress E-box-driven transcription clock-associated genes. There two individual CRY1 exhibiting higher affinity than CRY2. It is known that this differential binding regulated a dynamic serine-rich loop adjacent secondary pocket both but...
Human clock-gene variations contribute to the phenotypic differences observed in various behavioral and physiological processes, such as diurnal preference, sleep, metabolism, mood regulation, addiction, fertility. However, little is known about possible effects of identified at molecular level. In this study, we performed a functional characterization cellular level rare cryptochrome 2 (CRY2) missense that were from Ensembl database. Our structural studies revealed three (p.Pro123Leu,...
AbstractCryptochromes (CRYs) are essential components of the molecular clock that generates circadian rhythm. They inhibit BMAL1/CLOCK-driven transcription at level. There two CRYs have differential functions in mammals. It is not precisely known how they achieve such functions. In this study, we performed dynamic simulations on eight CRY mutants been experimentally shown to exhibit reduced repressor activities. Our results revealed mutations CRY1 affect behavior serine loop and availability...
<title>Abstract</title> Our goal was to investigate mitochondrial damage in a three-dimensional (3D) neural stem cell (NSC) organoid model using oxidative stress-induced NSCs as primary research method. To create an vitro model, we utilized that were exposed stress by treating them with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) at concentration of 75 µM, leading damage. Markers for stress, differentiation, and neurodegenerative diseases analyzed characterize models assessing gene...
Abstract Cryptochromes (CRYs), transcriptional repressors of the circadian clock in mammals, inhibit cAMP production when glucagon activates G-protein coupled receptors. Therefore, molecules that modulate CRYs have potential to regulate gluconeogenesis. In this study, we discovered a new molecule called TW68 interacts with primary pockets mammalian CRY1/2, leading reduced ubiquitination levels and increased stability. cell-based rhythm assays using U2OS: Bmal1 -d Luc cells, extended period...