A5021326087- Genetics, Aging, and Longevity in Model Organisms
- Air Quality and Health Impacts
- Climate Change and Health Impacts
- DNA Repair Mechanisms
- RNA Research and Splicing
- Heart Rate Variability and Autonomic Control
- GDF15 and Related Biomarkers
- Reproductive Biology and Fertility
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- Ion Channels and Receptors
- Noise Effects and Management
- Vibrio bacteria research studies
- Photosynthetic Processes and Mechanisms
- Medical and Biological Ozone Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Air Quality Monitoring and Forecasting
- Escherichia coli research studies
- Spaceflight effects on biology
University of Oregon
2020-2024
University of North Carolina at Chapel Hill
2014-2018
Indiana University School of Medicine
2017
Center for Systems Biology
2012-2014
Duke University
2011-2014
Sexually reproducing organisms use meiosis to generate haploid gametes and faithfully transmit their genome the next generation. In comparison oogenesis in many organisms, spermatogenesis is particularly sensitive small temperature fluctuations, spermatocytes must develop within a very narrow isotherm [1-4]. Although failure thermoregulate spermatogenetic tissue prolonged exposure elevated temperatures are linked male infertility several mechanisms of temperature-induced have not been fully...
Fluctuations in nutrient availability profoundly impact gene expression. Previous work revealed postrecruitment regulation of RNA polymerase II (Pol II) during starvation and recovery Caenorhabditis elegans, suggesting that promoter-proximal pausing promotes rapid response to feeding. To test this hypothesis, we measured Pol elongation genome wide by two complementary approaches analyzed conjunction with binding We confirmed bona fide also discovered docking. Pausing occurs at active...
Insulin-like signaling regulates developmental arrest, stress resistance and lifespan in the nematode Caenorhabditis elegans. However, genome encodes 40 insulin-like peptides, regulation function of individual peptides is largely uncharacterized. We used nCounter platform to measure mRNA expression all as well receptor daf-2, its transcriptional effector daf-16, daf-16 target gene sod-3. validated using 53 RNA samples previously characterized by high density oligonucleotide microarray...
Nutrient availability profoundly influences gene expression. Many animal genes encode multiple transcript isoforms, yet the effect of nutrient on isoform expression has not been studied in genome-wide fashion. When Caenorhabditis elegans larvae hatch without food, they arrest development first larval stage (L1 arrest). Starved can survive L1 for weeks, but growth and post-embryonic are rapidly initiated response to feeding. We used RNA-seq characterize transcriptome during over time after...
Studies have shown a relationship between air pollution and increased risk of cardiovascular morbidity mortality. Due to the complexity ambient composition, recent studies examined effects co-exposure, particularly particulate matter (PM) gas, determine whether pollutant interactions alter (e.g. synergistically, antagonistically) health response. This study examines independent fine (FCAPs) ultrafine (UFCAPs) concentrated particles on cardiac function, impact ozone (O₃) co-exposure We...
The preservation of genome integrity during sperm and egg development is vital for reproductive success. During meiosis, the tumor suppressor BRCA1/BRC-1 structural maintenance chromosomes 5/6 (SMC-5/6) complex genetically interact to promote high fidelity DNA double strand break (DSB) repair, but specific DSB repair outcomes these proteins regulate remain unknown. Using genetic cytological methods monitor resolution DSBs with different partners in Caenorhabditis elegans , we demonstrate...
Arranged in a spatial-temporal gradient for germ cell development, the adult germline of Caenorhabditis elegans is an excellent system understanding generation, differentiation, function, and maintenance cells. Imaging whole C. germlines along distal-proximal axis enables powerful cytological analyses nuclei as they progress from pre-meiotic tip through all stages meiotic prophase I. To enable high-content image analysis gonads, we developed custom algorithm pipelines to function with...
Abstract Arranged in a spatial-temporal gradient for germ cell development, the adult germline of Caenorhabditis elegans is an excellent system understanding generation, differentiation, function, and maintenance cells. Imaging whole C. germlines along distal-proximal axis enables powerful cytological analyses nuclei as they progress from pre-meiotic tip through all stages meiotic prophase I. To enable high-throughput image analysis gonads, we developed custom algorithm pipelines to function...