Proteolytic processing of paramyxovirus fusion (F) proteins is essential for the generation a mature and fusogenic form F protein. Although many are proteolytically processed by cellular protease furin at multibasic cleavage motif, newly emerged Hendra virus protein occurs previously unidentified following single lysine residue 109. We demonstrate here that cathepsin L involved in converting precursor (F(0)) to active F(1) + F(2) disulfide-linked heterodimer. To initially identify class...

More than 140 post-transcriptional modifications (PTMs) are known to decorate cellular RNAs, but their incidence, identity and significance in viral RNA still largely unknown. We have developed an agnostic analytical approach comprehensively survey PTMs on RNAs. Specifically, we used mass spectrometry analyze total isolated from cells infected with Zika virus, Dengue hepatitis C virus (HCV), poliovirus human immunodeficiency type 1. All five viruses significantly altered global PTM...

Abstract Despite the clear importance of left‐handed polyproline II (PPII) helical conformation in many physiologically important processes as well its potential significance protein unfolded states, little is known about physical determinants this conformation. We present here a scale relative PPII helix‐forming propensities measured for all residues, except tyrosine and tryptophan, proline‐based host peptide system. Proline has highest propensity system, result strong steric interactions...

Many viruses inhibit SGs. In this study, we observed that ZIKV restricts SG assembly, likely by relocalizing and subverting specific proteins to modulate replication. This ZIKV-SG protein interaction is interesting, as many are also known function in neuronal granules, which critical neural development function. Moreover, dysregulation of different neurons has been shown play a role the progression neurodegenerative diseases. The consequences modulating assembly alterations cellular mRNA...

Detection of viruses is critical for controlling disease spread. Recent emerging viral threats, including Zika virus, Ebola and SARS-CoV-2 responsible coronavirus 2019 (COVID-19) highlight the cost difficulty in responding rapidly. To address these challenges, we develop a platform low-cost rapid detection RNA with DNA nanoswitches that mechanically reconfigure response to specific viruses. Using virus as model system, show nonenzymatic selective multiplexed between related strains. For...

The alternative splicing of pre-mRNAs expands a single genetic blueprint to encode multiple, functionally diverse protein isoforms. Viruses have previously been shown interact with, depend on, and alter host machinery. consequences, however, incited by viral infection on the global slicing (AS) landscape are under-appreciated. Here, we investigated transcriptional profile neuronal cells infected with contemporary Puerto Rican Zika virus (ZIKVPR) isolate, an isolate prototypical Ugandan ZIKV...

ABSTRACT Zika virus (ZIKV) is a re-emerging mosquito-borne flavivirus that can have devastating health consequences. The developmental and neurological effects of ZIKV infection arise in part from the triggering cellular stress pathways perturbing transcriptional programs. To date, underlying mechanisms control directing viral restriction virus-host interaction are understudied. Activating Transcription Factor 3 (ATF3) stress-induced effector modulates expression genes involved myriad...

Sixty-seven rotavirus-positive fecal samples, collected between January and April 1999, from children with diarrhea in the Upper East Region of Ghana were examined for rotavirus VP7 VP4 types. Sufficient viral RNA could be obtained 46 (68.7%) samples all isolates had short electrophoretic pattern typed as subgroup I rotaviruses by ELISA. Three strains G8 specificity identified first time Ghana. G P typing PCR two distinct strains, P[6]G2 (50%) P[6]G8 (4.3%). Eighty-two percent (n = 38)...

Rotavirus strains circulating in Sousse, Tunisia, between 1995 and 1999 were characterized antigenically by monoclonal antibodies to the VP6 subgroup VP7 serotype. The VP4 genotype was determined reverse transcription-PCR, as with untyped VP7. Only 17% of 375 children shedding rotavirus latex agglutination assay. Most G1P[8] (50%), followed G4P[8] G4P[6]. Reassortant G1P[4] emerged Sousse during 1998–1999 season.

ABSTRACT Proteolytic cleavage of the Hendra virus fusion (F) protein results in formation disulfide-linked F 1 and 2 subunits, with occurring after residue K 109 sequence GDVK↓L. This unusual site efficient propagation a furin-deficient cell line indicate that is not cleaved by furin, protease responsible for proteolytic activation many viral proteins. To identify subcellular processing, Vero cells transfected pCAGGS-Hendra or pCAGGS-SV5 were metabolically labeled chased absence presence...

The Hendra virus fusion (F) protein contains five potential sites for N-linked glycosylation in the ectodomain. Examination of F mutants with single asparagine-to-alanine mutations indicated that two F(2) subunit (N67 and N99) F(1) (N414 N464) normally undergo glycosylation. While modification at N414 is critical folding transport, proteins lacking carbohydrates N67, N99, or N464 remained fusogenically active. As lies within heptad repeat B, these results contrast those seen several...

The transcriptional regulator deformed epidermal autoregulatory factor 1 (DEAF1) has been suggested to play a role in maintaining peripheral tolerance by controlling the transcription of tissue antigen genes lymph node stromal cells (LNSCs). Here, we demonstrate that DEAF1 also regulates translation LNSCs poorly characterized eukaryotic initiation 4 gamma 3 (Eif4g3) encodes eIF4GII. Eif4g3 gene expression was reduced pancreatic nodes Deaf1-KO mice, non-obese diabetic and type diabetes...

Zika virus (ZIKV), a re-emerging flavivirus, is associated with devasting developmental and neurological disease outcomes particularly in infants infected utero. Towards understanding the molecular underpinnings of unique ZIKV pathologies, numerous transcriptome-wide studies have been undertaken. Notably, these overlooked assimilation RNA-seq analysis from ZIKV-infected patients cell culture model systems. In this study we find that ZIKV-infection human lung adenocarcinoma A549 cells,...

Epidemiological studies on the VP7 serotype prevalence of human rotaviruses in South Africa and United Kingdom identified several strains which could not be serotyped as G1-G4 by monoclonal antibodies. Further analysis these with a G8-specific antibody probes for confirmed them G8 rotaviruses. These exhibited high degree sequence identity when compared each other rotavirus strains. Five African were further characterized VP6 subgroup I, but long RNA electropherotype, is similar to previously...

Over the past decade, a G4P[6] strain has been found to be circulating in different neonatal wards Pretoria area. This endemic was associated with both asymptomatic and symptomatic infection, providing opportunity undertake molecular study of some putative "virulence" genes. The genes encoding NSP4, VP8*, VP7 two Symptomatic strains were sequenced compared ST3. Within each these genes, amino acid substitutions unique South African recorded. Four conserved differences between at aa 82 (serine...

Zika virus (ZIKV) is a re-emerging mosquito-borne flavivirus that can have devastating health consequences. The developmental and neurological effects from ZIKV infection arise in part the triggering cellular stress pathways perturbing transcriptional programs. To date, underlying mechanisms of control directing viral restriction virus-host interaction are understudied. Activating Transcription Factor 3 (ATF3) stress-induced effector modulates expression genes involved myriad processes,...

The protein DDX3X is a DEAD-box RNA helicase that essential for the hepatitis C virus (HCV) life cycle. HCV core has been shown to bind both in vitro and vivo. However, specific interactions between these two proteins functional importance of viral cycle remain unclear. We show amino acids 16-36 near N-terminus interact specifically with Replication replicon NNeo/C-5B (genotype 1b) significantly suppressed HuH-7-derived cells expressing green fluorescent (GFP) fusions residues 16-36, but not...

Zika virus (ZIKV), a re-emerging flavivirus, is associated with devasting developmental and neurological disease outcomes particularly in infants infected