We have succeeded in culturing dermal papilla (DP) cells long term and developed new techniques that enhance their hair follicle-inducing efficiency a patch assay. The outgrowing DP from mouse vibrissae were markedly stimulated by 10% fetal bovine serum–Dulbecco's modified essential medium included fibroblast growth factor-2 (FGF-2). Moreover, the potency of proliferation was maintained during serial cultivations (more than 30 passages). combined these established with epidermal implanted...

ABSTRACT Nocardioides sp. strain KP7 grows on phenanthrene but not naphthalene. This organism degrades via 1-hydroxy-2-naphthoate, o -phthalate, and protocatechuate. The genes responsible for the degradation of to -phthalate ( phd ) were found by Southern hybridization reside chromosome. A 10.6-kb DNA fragment containing eight was cloned sequenced. phdA , phdB phdC phdD genes, which encode α β subunits oxygenase component, a ferredoxin, ferredoxin reductase, respectively, dioxygenase...

Abstract Objective Dry skin occurs in the winter season when relative humidity is low; however, mechanism by which low induces dry not fully understood. To develop measures against winter, understanding early changes upon exposure to essential. This study employed a reconstructed human epidermal model (RHEM) understand these changes. Methods The stratum corneum (SC) of RHEM was exposed low‐humidity air for 1 h, and trans‐epidermal water loss (TEWL) penetration rate lucifer yellow (LY) were...

1-Hydroxy-2-naphthoate dioxygenase, which cleaves the singly hydroxylated aromatic ring, was purified from phenanthrene-degrading Nocardioides sp. strain KP7. The enzyme had a molecular mass of 45 kDa by SDS-polyacrylamide gel electrophoresis and 270 filtration chromatography. apparent Km kcat values this for 1-hydroxy-2-naphthoate were 10 μm 114 s−1, respectively. One mole oxygen consumed when 1 mol oxidized. This contained Fe(II)/mol subunit inactivated o-phenanthroline. that been...

2-Carboxybenzaldehyde dehydrogenase from the phenanthrene-degrading bacterium Nocardioides sp. strain KP7 was purified and characterized. The enzyme had a molecular mass of 53 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis 205 filtration chromatography. Thus, homotetramer 53-kDa subunit constituted an active enzyme. apparent Km kcat values this for 2-carboxybenzaldehyde were 100 microM 39 s(-1), respectively, those NAD+ 83 32 respectively. structural gene cloned sequenced....

Background Androgenetic alopecia (AGA) is the most common type of baldness in men. The balding process associated with gradual miniaturization hair follicles and successive loss. However, relative contributions density diameter to AGA are still unclear. Objectives Hair were investigated Japanese men or without elucidate importance these factors process. Methods Male subjects (n = 369) included this study. appearance at vertex was evaluated by comparison a series standard photographs....

Studies showing that Panax ginseng promotes hair growth have largely been conducted using mice; there are few reports on how P. affects human growth. In particular, little is known about its effect the telogen to anagen transition. To determine of and transition from phase. The effects extract (PGE) three major ginsenoside components, Rb1, Rg1, Re, proliferation dermal papilla cells (DPCs) outer root sheath (ORSCs) were investigated. these compounds cell expression bone morphogenetic protein...

18-β-Glycyrrhetinic acid, a major component of licorice, stimulated the proliferation both dermal papilla cells and outer root sheath isolated from human hair follicles. Thus, suggesting that this compound promotes growth. Furthermore, inhibited activity testosterone 5α-reductase, an enzyme responsible for converting androgen to dihydroandrogen, with IC

ABSTRACT 1-Hydroxy-2-naphthoate (compound I) is a metabolite of the phenanthrene-degradative pathway in Nocardioides sp. strain KP7. This singly hydroxylated aromatic compound cleaved by 1-hydroxy-2-naphthoate dioxygenase. In this study, structure ring cleavage product generated action homogeneous dioxygenase was determined upon separation high-performance liquid chromatography at pH 2.5 using nuclear magnetic resonance (NMR) and mass spectroscopic techniques. The existed equilibrium between...

The distribution of oil-degrading microorganism in samples surface water and sediment from North Eastern Japanese coastal waters was studied. Modified natural sea (NSW) agar supplemented with emulsified crude oil (Arabian light, 5 g 1−1) used to enumerate bacteria. In addition, filtered were inoculated into NSW broth containing weathered oil. Incubation carried out at 20°C for 7–10 days. Populations microorganisms ranged 3–230 CFU 100 ml−1 2.9 × 103 1.2 105 g−1 samples. Analysis variance...

Androgenetic alopecia (AGA) is a hair loss disorder that commonly affects middle-aged men. To date, the properties of number natural or synthetic substances have been investigated for their ability to improve condition.To evaluate growth-promoting activities an extract from root Sophora flavescens Aiton.We used human keratinocyte proliferation assay and ex vivo organ cultures follicle examine potential stimulate growth via anagen elongation. We isolated compounds promoting epithelial cells,...

Lanceolate nerve endings (LNEs) surrounding hair follicles (HFs) play an important role in detecting deflection. Complexes of the LNEs form a palisade-like structure along longitudinal axis roots which axons are sandwiched between two processes terminal Schwann cells (tSCs) at isthmus HFs. The and molecular mechanism animal sinus hair, pelage, human vellus hairs have been investigated. Despite high density HFs scalp skin, not In this study, we aimed to reveal distribution ultrastructure...

trans-2'-Carboxybenzalpyruvate hydratase-aldolase was purified from a phenanthrene-degrading bacterium, Nocardioides sp. strain KP7, and characterized. The enzyme found to have molecular masses of 38 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis 113 filtration chromatography. Thus, the homotrimer 38-kDa subunit constituted an active enzyme. Km kcat values this for trans-2'-carboxybenzalpyruvate were 50 microM 13 s(-1), respectively. transformed 2-carboxybenzaldehyde...

The method to internally label PCR products with multiple colored fluorescent dyes was developed and applied fluorescence-based single-stranded conformational polymorphism (MF-PCRSSCP) analysis. PCR-amplified DNA fragments, which were labeled by adding dUTPs ([F]dUTPs) the mixture, heatdenatured a nondenaturing polyacrylamide gel (SSCP gel) set on an automated sequencer temperature-controlling system. image data analyzed GENESCANTM 672 software . In spite of differences in species amount...

The structural genes for the entire bacitracin synthetase 2 (component II) and a part of putative 3 III) from Bacillus licheniformis ATCC 10716 were cloned expressed in Escherichia coli. A cosmid library B. DNA was constructed. screened ability to produce by situ immunoassay using anti-bacitracin antiserum. positive clone designated B-15, which has recombinant plasmid carrying about 32-kilobase insert DNA, further characterized. Analysis crude cell extract B-15 polyacrylamide gel...

Abstract Adenosine is an effective treatment for androgenetic alopecia ( AGA ) in Japanese men and women. exerts its effects by significantly increasing the proportion of thick hair. In this study, we assessed clinical outcome adenosine 6 months 38 Caucasian men. The change hair (≥60 μm) compared with baseline group was higher than that placebo P < 0.0001). vellus (<40 lower = 0.0154). density also 0.0470). No adverse due to were noted during study dermatological evaluation. as well

Hair thickness is more important than hair density in the appearance of baldness male with androgenetic alopecia (AGA). Adenosine improves loss by stimulating growth and thickening shafts women. The objective this study was to evaluate efficacy safety topical adenosine men AGA.A lotion containing either or niacinamide administered scalps 102 Japanese twice daily for 6 months a double-blind, randomized study. Efficacy evaluated dermatologists who assessed quality calculating percentages...

The purpose of this study was to clarify the impact protein carbonylation on chemical characteristics hair surface focusing hydrophobicity.First, we examined validity methods evaluate hydrophobicity, one that utilizes fluorescence 1-anilinonaphtalene-8-sulfonic acid (1,8-ANS) compared with contact angles against H2 O, chemically modified by alkaline hydrolysis or treated stearyl ammonium chloride. We measured hairs bleached O2 acrolein for originating from 1,8-ANS, angle and changes...