Controlling the bulk and surface properties of materials is a real challenge for bioengineers working in fields biomaterials, tissue engineering biophysics. The layer-by-layer (LbL) deposition method, introduced 20 years ago, consists alternate adsorption polyelectrolytes that self-organize on material's surface, leading to formation polyelectrolyte multilayer (PEM) films.1 Because its simplicity versatility, procedure has led considerable developments biological applications within past 5...

Hyaluronic acid (HA) is a natural polysaccharide abundant in biological tissues with excellent potential for constructing synthetic extracellular matrix analogues. In this work, we established simple and dependable approach to prepare hyaluronic acid-based hydrogels controlled stiffness cell recognition properties use as cell-interactive substrates. This relied on new procedure the synthesis of methacrylate-modified HA macromers (HA-MA) and, photorheometry allowing real time monitoring...

Polyelectrolyte microcapsules were prepared by the layer-by-layer assembly of hyaluronic acid (HA) and a polycationic polymer, poly(allylamine) (PAH) or poly(lysine) (PLL). The influence partner on morphology, stability, permeability properties, enzymatic degradation was thoroughly analyzed. It found that these properties could be tuned shell cross-linking. Confocal microscopy studies cellular uptake capsules showed polyelectrolyte shells remain stable outside cells but readily break open...

Significance A basic requirement in biomedical research is the ability to specifically target cells and tissues. Targeting typically relies on specific binding of a “ligand” tailor-made probe “receptor” desired cell/tissue. Conventional probes efficiently distinguish biological entity displaying receptor from others that do not, but exhibit limited selectivity when entities be distinguished display given at different densities. Multivalent bind several receptors simultaneously potentially...

Despite their importance for material and life sciences, multivalent interactions between polymers surfaces remain poorly understood. Combining recent achievements of synthetic chemistry surface characterization, we have developed a well-defined highly specific model system based on host/guest interactions. We use this to study the binding hyaluronic acid functionalized with host molecules tunable displaying different densities guest molecules. Remarkably, find that density bound polymer...

Abstract Chitosan ( CS ) has received much attention as a functional biopolymer for designing various hydrogels biomedical applications. This review provides an overview of the different types ‐based hydrogels, approaches that can be used to fabricate hydrogel matrices with specific features and their applications in controlled drug delivery tissue engineering. Emphasis is laid on recent design concepts hybrid based mixtures natural or synthetic polymers, interpenetrating polymer networks...

We demonstrate here, for the first time, formation of injectable dynamic covalent hydrogels at physiological pH using benzoxaborin-saccharide complexation as a reversible cross-linking method. The gels were prepared by simply mixing hyaluronic acid modified with an original boronic derivative, 3,4-dihydro-2H-benzo[e][1,2]oxaborinin-2-ol (1,2-ABORIN), and HA functionalized 1-amino-1-deoxy-d-fructose. Dynamic rheological experiments confirmed gel-like behavior (storage modulus (G′) > loss (G″)...

The term liquid crystal is often associated with fascinating compounds that exhibit unusual melting or solubilisation properties, however, it also represents a unique collection of mesophases exist between the solid state and amorphous liquid. As such, this matter can be accessed by wide variety materials from low molar mass to polymeric systems. In Feature Article we describe some recent studies concerning liquid-crystalline behaviour 'in-between' have discrete molecular structures, which...

Dynamic covalent hydrogels are facilely prepared from biocompatible polysaccharides in physiological conditions by the formation of phenylboronate ester cross-links. This is based on simple mixing carboxylate-containing (i.e., hyaluronic acid or carboxymethylcellulose) modified with phenylboronic and maltose moieties according to mild coupling reactions performed aqueous solution. The dynamic networks reversible boronic-ester cross-links demonstrated analyzing their rheological behavior....

Chronic UVB exposure promotes oxidative stress, directly causes molecular damage, and induces aging-related signal transduction, leading to skin photoaging. Dihydrocaffeic acid (DHCA) is a phenolic compound with potential antioxidant capacity thus promising for the prevention of UVB-induced photodamage. The aim this study was evaluate protective effect DHCA against apoptosis, matrix metalloproteinase (MMP) expression via mitogen-activated protein kinase (MAPK) signaling pathway on L929...

The grafting of a cholesterol derivative onto methylated cyclodextrin through spacer arm produces an amphiphilic compound exhibiting high solubility in water. This new molecule was fully characterized terms chemical and optical purities by resolution NMR mass spectrometry. An analysis its behavior aqueous solution using surface tension measurements light, small-angle X-ray, neutron scattering techniques proved that it self-assembles into monodisperse spherical micelles with average...

Different types of polyelectrolyte multilayer films composed poly(l-lysine)/hyaluronan (PLL/HA), chitosan/hyaluronan (CHI/HA) and poly(allylamine hydrochloride)/poly(l-glutamic acid) (PAH/PGA) have been investigated for their internal composition, including water content, ion pairing, ability to be covalently cross-linked, as well mechanical properties. Film buildup under physiological conditions was monitored by the quartz crystal microbalance with dissipation monitoring (QCM-D) attenuated...

We report the synthesis of fully biodegradable polymeric nanoparticles presenting mannose residues at their surface and interaction with lectins. A simple versatile method was used to reach functionalization poly(D,L-lactic acid) (PLA) by moieties: It consists in using an amphiphilic mannosylated poly(ethylene oxide)-b-poly(E-caprolactone) (PEO-b-PCL) diblock copolymer as a bioresorbable modifier nanoprecipitation-evaporation procedure. The size zeta potential were found depend on molar...

Abstract We present herein a mild and rapid method for the modular functionalization of polysaccharides. Several ene‐functional charged neutral polysaccharides, that is, hyaluronic acid dextran, were prepared by esterification hydroxyl groups with pentenoic anhydride. The modified polysaccharides then reacted six model mercaptans under UV light, leading to linear polymers hydrophobic groups, peptides, or oligosaccharides as well chemical hydrogels. thiol‐ene coupling reactions found proceed...

A novel type of biocompatible hollow capsules that combine severable favorable features as a hydrophobic drug carrier, including host–guest complexation in the shell, unique biological functions hyaluronic acid (HA), and transport properties multilayer was designed prepared. These were generated by layer-by-layer (LbL) deposition HA modified with β-cyclodextrin (CD) molecules poly(l-lysine) (PLL) on calcium carbonate particles. Simultaneously, paclitaxel (PTX) loaded LbL wall via...

A hyaluronic acid (HA)-based extracellular matrix (ECM) platform with independently tunable stiffness and density of cell-adhesive peptide (RGD, arginine-glycine-aspartic acid) that mimics key biochemical mechanical features brain has been designed. We demonstrated here its utility in elucidating ECM regulation neural progenitor cell behavior neurite outgrowth. The analysis outgrowth 3-D by two-photon microscopy showed several important results the development these hydrogels. First, ability...

New supramolecular assemblies based on a chitosan bearing pendant cyclodextrins were prepared. For this purpose, adamantyl groups which can selectively be included in the cyclodextrin cavity grafted and various poly(ethylene glycol)s (Mn = 3400, 6000, 20000) affording guest macromolecules with different structural features. The specific interaction of latter CD−chitosan through inclusion complex formation was examined by rheological measurements. Physical cross-linking between chains shown...

Abstract It is shown that blend multilayers of hyaluronan (HA) and heparin (HEP) as polyanions poly(L‐lysine) (PLL) a polycation can be used to prepare films with different thicknesses chemical compositions. The amounts recombinant human BMP‐2 (rhBMP‐2) loaded the fraction initially released from depend on film's composition. rhBMP‐2 in are much higher for HA mass fractions more than 0.4. bioactivity rhBMP‐2‐loaded investigated C2C12 myoblasts, which differentiates into osteoblasts contact...

Abstract Polyelectrolyte‐multilayer microcapsules are made by layer‐by‐layer (LbL) assembly of oppositely charged polyelectrolytes onto sacrificial colloidal particles, followed core removal. In this paper, contact‐killing polyelectrolyte prepared based solely on polysaccharides. To end, water‐soluble quaternized chitosan (QCHI) with varying degrees substitution (DS) and hyaluronic acid (HA) assembled into thin films. The quaternary ammonium groups selectively grafted the primary amine group...