A5043024267- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Reproductive Biology and Fertility
- Organ and Tissue Transplantation Research
- Birth, Development, and Health
- Pregnancy and preeclampsia studies
- Genetics, Aging, and Longevity in Model Organisms
- Liquid Crystal Research Advancements
- Human Health and Disease
- Sperm and Testicular Function
- Flame retardant materials and properties
- Aquaculture Nutrition and Growth
- nanoparticles nucleation surface interactions
- Neuroendocrine regulation and behavior
- Fish Ecology and Management Studies
- Reproductive Health and Technologies
- Bone fractures and treatments
- Neurogenesis and neuroplasticity mechanisms
- Medical and Agricultural Research Studies
- Reproductive biology and impacts on aquatic species
- Agriculture and Biological Studies
- Polysaccharides and Plant Cell Walls
- Renal and related cancers
- Pluripotent Stem Cells Research
- Coal Combustion and Slurry Processing
University of South Bohemia in České Budějovice
2024
National Academy of Sciences of Ukraine
2013-2021
Institute for Problems of Cryobiology and Cryomedicine
2013-2021
There have been studied morphological safety and functional state of the explants human placenta, umbilical cord fetal membranes by vital staining techniques using MTT resazurin reduction tests, level glucose in incubation medium, activity lactate dehydrogenase alkaline phosphatase before after cryopreservation. It has found that proposed program cryopreservation allows keeping a high viability membranes, most informative methods assessing these biological objects are method staining,...
The paper covers the actual tasks in low temperature storage of such placental biological derivatives as: cell-free material, cell suspensions, tissue, umbilical cord and fetal membranes. Depending on purposes for specimen main research directions this field are as follows: diagnostic, experimental, clinical, establishment donor or personal bank. Nowadays a top priority is designing methods freeze-drying, hypothermic storage, cryopreservation with different regimens. Probl Cryobiol Cryomed...
According to the WHO data, date population lifespan is increasing worldwide due infant mortality decrease, rather than duration and life quality of elderly people. Most effective geroprotectors affect immune nervous systems, cellcycle, metabolism carbohydrates, stimulate autophagy. Bioobjects placental origin possess similar properties. The research aim was study effect cryopreserved human explants on BALB/c mice. Kaplan-Meier survival curves were analyzed indices calculated. differences...
Abstract The primary function of spermatozoa is to fertilize the oocyte, which depends on their motility and directly associated with metabolic state. oxygen consumption rate (OCR) reflects respiratory capacity sperm mitochondria under various physiological conditions an essential marker quality. We determined OCR common carp ( Cyprinus carpio ) using two respirometry methods: conventionally used polarographic method a Clark-type electrode fluorometric assay Oxo Dish optochemical sensor....
Експериментально дослідили вплив імплантації кріоконсервованих фрагментів плаценти людини (КФП) на статеву систему і репродуктивну функцію самиць щурів, що старіють.Виявлено збільшення числа зростаючих зрілих фолікулів удвічі, жовтих тіл -в 1,8 раза порівняно зі старими хибнооперованими самицями.Спостерігалося зниження апоптотичного індексу клітинних елементів яєчників, оптичної щільності світіння колагену IV типу та інтенсивності ендотеліоцитів, експресують рецептори до ендотеліну-1.Після...
High efficacy of placental cells application necessitates their investigation. Preclinical studies require an improvement the methods for obtaining, standardizing and storage experimental animals. Cells were isolated from rats mice placentas by means different enzymatic one explants. cryopreserved with DMSO in DMEM using two-stage freezing. The number, morphological, cultural, metabolic features studied after isolation storage. maximum number viable was found to be obtained explant method or...
Premature ovarian insufficiency (POI) is condition of female hypogonadism. One the reasons for development POI anticancer chemotherapy. Currently, hormone replacement therapy considered most effective method treatment POI, which, however, had a number side effects, requires constant administration pharmaceuticals, did not restore fertility and correct pathological changes in target organs. Therefore, search alternative methods based on use stem cells promising. The aim work was to compare...
Background. Development and improvement of methods treating patients with a mine-blast trauma (MBT) involves the experiments animals. A particular way to simulate MBT in vivo determines nature range injury severity as well their possible regulation. Objective. Morphological evaluation rat femoral tissue injuries following depending on powder charge energy availability strike elements. Methods. The modelling was done cartridge-operated guns. inflicted wounds were histologically studied at 7th...
Experimental myocardial necrosis course during alginate hydrogel implantation, saturated with
Клітини, тканини, біологічно активні речовини плаценти є перспективними об’єктами для ефективного лікування патологічних станів ендокринної, імунної та нервової систем. Для клінічного застосування похідних необхідна розробка методів їх короткотривалого зберігання і транспортування від клініки до біотехнологічної лабораторії в зворотному напрямку.
 Мета дослідження – визначити можливості субнормотермічного (+20 ºС) гіпотермічного (+4 експлантів середовищі культивування використання у...