A5072972414- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Transgenic Plants and Applications
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Advanced Drug Delivery Systems
- Essential Oils and Antimicrobial Activity
- Nanocomposite Films for Food Packaging
Kyushu University
2022-2025
The rising incidence of fungal infections, compounded by the emergence severe antifungal resistance, has resulted in an urgent need for innovative therapies. We developed protein-based formulation as a topical agent combining artificial lipidated chitin-binding domain chitinase (LysM-lipid) with recently ionic liquid-in-oil microemulsion formulations (MEFs). Our findings demonstrated that lipid moieties attached to LysM and MEFs effectively disrupted integrity stratum corneum mouse skin...
Fungal infections affect more than one billion people worldwide and cause million deaths per year. Amphotericin B (AmB), a polyene antifungal drug, has been used as the gold standard for many years because of its broad spectrum, high activity, low tendency drug resistance. However, side effects AmB, such nephrotoxicity hepatotoxicity, have hampered widespread use, leading to development liposome-type AmB formulation, AmBisome. Herein, we report simple but highly effective strategy enhance...
Candida albicans is a prevalent fungal pathogen that displays antibiotic resistance. The polyene antifungal amphotericin B (AmB) has been the gold standard because of its broad spectra, and liposomal formulation, AmBisome, used widely clinically in treating infections. Herein, we explored enhancing activity AmBisome by integrating small chitin-binding domain (LysM) chitinase A derived from Pteris ryukyuensis. LysM conjugated with lipid (LysM-lipid) was initially prepared through microbial...
The antifungal activity of a small chitin-binding domain from chitinase was enhanced by the artificial lipidation with specific alkyl chain length and structure in presence amphotericin B.
Abstract Fungal infections have been a concern for decades, yet effective and approved antifungal agents are limited. We recently developed potential method to enhance the activity of small chitin-binding domain (LysM) from Pteris ryukyuensis chitinase A (PrChiA) by site-specific introduction palmitoyl (C16) group catalyzed microbial transglutaminase (MTG). Herein, we attempted conjugation series lipid-peptide substrates with LysM genetically fused C-terminal MTG-reactive Q-tag (LysM-Q)...