Human tear analysis is gaining increasing attention as a non-invasive tool for several applications such proteomics and biomarker identification in various diseases, including cancer. The choice of the correct sampling method determines result analysis. In this study, we developed validated robust protein quantification using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Tear samples were collected Schirmer strips, low-cost practical...

Aims/Purpose: The identification of biomarkers in tears help diagnosis and management ocular diseases, beyond [1]. We aimed to develop validate a method for extracting quantifying proteins from human collected using Schirmer test strips, which are typically discarded after clinical use. Methods: Tears 18 healthy volunteers were by aspiration with micropipette paper strips use (5 minutes at closed eyes). Aspirated centrifuged stored ‐80°C until processing. eluted developed extraction buffer...

Aims/Purpose: The identification of biomarkers in tears help diagnosis and management ocular diseases, beyond [1]. We aimed to develop validate a method for extracting quantifying proteins from human collected using Schirmer test strips, which are typically discarded after clinical use. Methods: Tears 18 healthy volunteers were by aspiration with micropipette paper strips use (5 minutes at closed eyes). Aspirated centrifuged stored ‐80°C until processing. eluted developed extraction buffer...

Aims/Purpose: This study investigates the potential of tear fluid analysis as a non‐invasive method to monitor air pollution impact on an exposed body tissue, represented by ocular surface [1,2]. In particular, we characterized particulate matter (PM) in tears healthy volunteers or not urban environment. Methods: Twenty were enrolled from personnel staff, ten traffic Bologna, Italy period January‐April 2024 for minimum 2 hours, and not. A 5 μL sampled lower conjunctival sac, deposited onto...

Aims/Purpose: The treatment of ocular surface diseases (OSD) requires the administration therapeutic agents in sufficient quantities for proper time to reach efficacy and relieve from subjective symptoms. Delivery systems avoid systemic routes which may require undesirable concentrations appropriate levels at target site. aim this work was standardize two polyester‐based co‐polymers ophthalmic application. Methods: cytotoxicity, morphological changes, adhesion behaviour copolymers study were...

Aims/Purpose: The loss of ocular surface homeostasis leads to the Dry Eye Disease (DED) onset. Although tear substitutes are front‐line treatment for DED, recent advances suggest new therapeutic approaches modulate different mechanisms leading impairment [1]. For this reason, there is a need better understand specific interactions between formulations and native components tears. This pilot study focused on assessing chemical stability two major human proteins, lysozyme C (LYS‐C) lactoferrin...

Lactoferrin (Lf), a multifunctional protein found abundantly in secretions, including tears, plays crucial role ocular health through its antimicrobial, immunoregulatory, anti-inflammatory, and antioxidant activities. Advanced delivery systems are desirable to fully leverage therapeutic potential treating diseases. The process of Lf quantification for diagnostic purposes underscores the importance developing reliable, cost-effective detection methods, ranging from conventional techniques...

Dry eye disease (DED) is a multifactorial where ocular surface inflammation and damage play key etiological roles. To compare combination of 3% trehalose (T) 0.15% hyaluronic acid (HA) (Thealoz duo®, T/HA) with tear substitute containing 0.001% hydrocortisone (I) 0.2% HA (Idroflog®, I/HA), respect to changes on signs inflammatory markers in mouse DED model. Thirty 12-week-old C57BL/6 mice were exposed controlled-environment chamber as desiccating stress model for 35 days. At day 14 (T1),...

The aim of this study was to optimize a coculture in vitro model established between the human Müller glial cells and umbilical vein endothelial cells, mimicking inner blood-retinal barrier, explore its resistance damage induced by oxidative stress. A spontaneously immortalized cell line MIO-M1 (HUVEC) were plated together at density ratio 1:1 maintained up 8th passage (p8). MIO-M1/HUVECs p1 through p8 treated with increasing concentrations (range 200-800 μM) H2 O2 evaluate stress comparing...

Blood-based preparations are used in clinical practice for the treatment of several eye disorders. The aim this study is to analyze effect freeze-drying blood-based on levels growth factors and wound healing behaviors an vitro model. Platelet-rich plasma (PRP) serum (S) from same Cord Blood (CB) sample, prepared both fresh frozen (FF) freeze-dried (FD) forms (and then reconstituted), were analyzed EGF BDNF content (ELISA Quantikine kit). human MIO-M1 glial cell line (Moorfield/Institute...

Ocular graft-versus-host disease (oGVHD) is a manifestation of chronic GVHD, frequently occurring in patients after allogeneic hematopoietic stem cell transplant (HSCT). We analyzed tear protein changes before and HSCT, correlated their levels with the oGVHD development. This retrospective study included 102 patients, data were recorded conditioning treatment, 3 to 6 months postoperatively. Tear analysis was performed Agilent-2100 Bioanalyzer on individual tears sampled by aspiration. Total...