A5063696689- Drug Transport and Resistance Mechanisms
- Ocular Surface and Contact Lens
- Liver Diseases and Immunity
- Fibroblast Growth Factor Research
- Gut microbiota and health
- Pharmacological Effects and Toxicity Studies
- Phytochemicals and Antioxidant Activities
- Liver Disease Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Diet and metabolism studies
- Coenzyme Q10 studies and effects
- Effects and risks of endocrine disrupting chemicals
- Cerebrospinal fluid and hydrocephalus
- Phytochemical Studies and Bioactivities
- Dye analysis and toxicity
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects of Natural Compounds
- Skin Protection and Aging
- thermodynamics and calorimetric analyses
- Inflammatory mediators and NSAID effects
- Biochemical Acid Research Studies
- Air Quality Monitoring and Forecasting
- ATP Synthase and ATPases Research
- Pesticide Residue Analysis and Safety
- Redox biology and oxidative stress
University of Bologna
2017-2025
Istituto Nazionale Biostrutture e Biosistemi
2022-2025
Karolinska University Hospital
2022
BackgroundDisruption of bile acid (BA) homeostasis plays a key role in intestinal inflammation. The gut-liver axis is the main site for regulation BA synthesis and pool size via combined action nuclear Farnesoid X Receptor (FXR) enterokine Fibroblast Growth Factor 19 (FGF19). Increasing evidence have linked derangement metabolism with dysbiosis mucosal Thus, here we aimed to investigate potential an FGF19 analogue on microbiota inflammation.MethodsA novel engineered non-tumorigenic variant...
Marine mussels, especially Mytilus galloprovincialis, are well-established sentinel species, being naturally resistant to the exposure multiple xenobiotics of natural and anthropogenic origin. Even if response xenobiotic is well known at host level, role mussel-associated microbiome in animal environmental pollution poorly explored, despite its potential detoxification important development, protection, adaptation. Here, we characterized microbiome-host integrative M. galloprovincialis a...
Food waste is a global problem due to its environmental and economic impact, so there great demand for the exploitation of new functional applications. The winemaking process leads an incomplete extraction high-value compounds, leaving pomace still rich in polyphenols. This study was aimed at optimising validating sustainable routes toward further valorisation these polyphenols, particularly cosmeceutical New formulations based on red grape polyphenols natural deep eutectic solvents (NaDESs)...
The analysis of cosmetic products represents an important field analytical chemistry, since the demand for new formulations is continuously increasing. Regulations about prohibited/regulated compounds are applied in each country. Among substances that banned cosmetics, corticosteroids represent a potential harm consumers prolonged exposure to these can affect health status. However, be found cosmetics as illegal addition they able alleviate symptoms inflammatory skin problems. In this work,...
Aims/Purpose: The identification of biomarkers in tears help diagnosis and management ocular diseases, beyond [1]. We aimed to develop validate a method for extracting quantifying proteins from human collected using Schirmer test strips, which are typically discarded after clinical use. Methods: Tears 18 healthy volunteers were by aspiration with micropipette paper strips use (5 minutes at closed eyes). Aspirated centrifuged stored ‐80°C until processing. eluted developed extraction buffer...
Aims/Purpose: The identification of biomarkers in tears help diagnosis and management ocular diseases, beyond [1]. We aimed to develop validate a method for extracting quantifying proteins from human collected using Schirmer test strips, which are typically discarded after clinical use. Methods: Tears 18 healthy volunteers were by aspiration with micropipette paper strips use (5 minutes at closed eyes). Aspirated centrifuged stored ‐80°C until processing. eluted developed extraction buffer...
Aims/Purpose: This study investigates the potential of tear fluid analysis as a non‐invasive method to monitor air pollution impact on an exposed body tissue, represented by ocular surface [1,2]. In particular, we characterized particulate matter (PM) in tears healthy volunteers or not urban environment. Methods: Twenty were enrolled from personnel staff, ten traffic Bologna, Italy period January‐April 2024 for minimum 2 hours, and not. A 5 μL sampled lower conjunctival sac, deposited onto...
Human tear analysis is gaining increasing attention as a non-invasive tool for several applications such proteomics and biomarker identification in various diseases, including cancer. The choice of the correct sampling method determines result analysis. In this study, we developed validated robust protein quantification using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Tear samples were collected Schirmer strips, low-cost practical...
Critical regulation of bile acid (BA) pool size and composition occurs via an intensive molecular crosstalk between the liver gut, orchestrated by combined actions nuclear Farnesoid X receptor (FXR) enterokine fibroblast growth factor 19 (FGF19) with final aim reducing hepatic BA synthesis in a negative feedback fashion. Disruption homeostasis increased toxic levels leads to higher incidence hepatocellular carcinoma (HCC). While native FGF19 has anti-cholestatic anti-fibrotic activity liver,...
Altered bile acid (BA) signaling is associated with hepatotoxicity. The farnesoid X receptor (FXR) a nuclear that transcriptionally regulates BA homeostasis. Mice FXR ablation present hepatocarcinoma (HCC) due to high toxic levels. Abcb4 accumulate within the ducts and HCC. We have previously shown intestinal specific activation of by transgenic VP16-FXR chimera able reduce pool size prevent Here we tested chemical administering for 15 months dual FXR/ membrane G protein-coupled (TGR5)...
Accumulating evidence suggests that high consumption of natural antioxidants promotes health by reducing oxidative stress and, thus, the risk developing cardiovascular diseases. Similarly, fermentation compounds with lactic acid bacteria (LAB), such as Lactiplantibacillus plantarum, enhances their beneficial properties regulators immune, digestive, and system. We investigated effects plantarum on antioxidant immunomodulatory Pushgay berries (Vaccinium floribundum, Ericaceae family) in human...
The release of hazardous chemicals into aquatic environments has long been a known problem, but its full impact only recently realized. This study presents validated liquid chromatography–mass spectrometry (HPLC–MS/MS) method for detecting pharmaceutical and pesticide residues in mussels (Mytilus galloprovincialis). An innovative MS-compatible extraction was developed validated, demonstrating successful recovery rates analytes at three different concentration levels (25–95%). detected the...
Abstract There is a growing interest in the named “acidic sterolbiome” and genetic potential of gut microbiome (GM) to modify bile acid (BA) structure. Indeed, qualitative composition BAs feces correlates with bowel microorganisms their collective material. GM responsible for production BA metabolites, such as secondary oxo-BAs. The specific profiles, microbiome-host co-metabolic products, could be useful investigate GM-host interaction animals under physiological conditions, well diseases....
Bile acids (BAs), endogenous acidic steroids synthetized from cholesterol in the liver, play a key role gut-liver axis physiopathology, including hepatotoxicity, intestinal inflammatory processes, and homeostasis. Faecal Oxo-BAs, relatively stable intermediates of oxidation/epimerization reactions BA hydroxyls, could be relevant to investigating crosstalk liver-gut relationship between diseases alterations microbiota composition. A paucity information currently exists on faecal profiles dogs...
Background: Inflammatory bowel diseases (IBD), ulcerative colitis (UC), and Crohn’s disease (CD), represent systematic chronic conditions with a deficient intestinal absorption. We first attempt to investigate the serum bile acids (sBAs) profile in large cohort of IBD patients evaluate changes under anti-TNF alpha treatment. Methods: Forty CD 40 UC were enrolled BAs quantified by high-pressure liquid chromatography-electrospray-tandem mass spectrometry (HPLC-ES-MS/MS). Up 15 different sBAs...
The Farnesoid X Receptor (FXR) is the master regulator of Bile Acids (BA) homeostasis orchestrating their synthesis, transport and metabolism. Disruption BA regulation has been linked to gut-liver axis diseases such as colorectal cancer (CRC). In this study, firstly we examined role constitutive activation intestinal FXR in CRC; then pre-clinically investigated therapeutic potential a diet enriched with synthetic agonist two models CRC (chemically-induced genetic models). We demonstrated...
Abstract Small organic molecules, lipids, proteins, and DNA fragments can remain stable over centuries. Powerful sensitive chemical analysis therefore be used to characterize ancient remains for classical archaeological studies. This bio-ecological dimension of archaeology contribute knowledge about several aspects life, including social organization, daily habits, nutrition, food storage. Faecal (i.e. coprolites) are particularly interesting in this regard, with scientists seeking identify...
The fight against coronavirus disease 2019 (COVID-19) continues. Since the pandemic’s onset, several biomarkers have been proposed to assess diagnosis and prognosis of this disease. This research aimed identify potential severity in serum samples patients with COVID-19 during course. Data were collected using untargeted targeted mass spectrometry methods. results interpreted by performing univariate multivariate analyses. Important metabolite classes identified qualitative metabolomics 15...
Bile acids are known to pass the blood–brain barrier and present at low concentrations in brain. In a previous work, it was shown that subdural hematomas enriched with bile levels such higher than peripheral circulation. The mechanism behind this enrichment never elucidated. have high affinity albumin, contain almost as albumin A hematoma is encapsulated by fibrin which may allow passage of small molecules like acids. We hypothesized originating from circulation be ‘trapped’ hematomas. we...