A5010699615- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Adenosine and Purinergic Signaling
- Thermoregulation and physiological responses
- RNA Interference and Gene Delivery
- Cannabis and Cannabinoid Research
- Optical Imaging and Spectroscopy Techniques
- Pluripotent Stem Cells Research
- Diabetes Management and Research
- 3D Printing in Biomedical Research
- Circadian rhythm and melatonin
- Diet, Metabolism, and Disease
Public Health England
2017
Newcastle University
2008-2011
Abstract Increasing evidence regarding positive effects of exposure to sunlight has led suggestions that current advice may be overly weighted in favour avoidance. UV-A been reported lower blood pressure, possibly through nitric oxide (NO) production skin. Here, we set out investigate and solar-simulated radiation on the potential source dermal NO, effective doses wavelengths, responsiveness different human skin cells, magnitude inter-individual differences influence age. We utilised...
PFK-2/FBPase-2 (6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase) catalyses the formation and degradation of fructose 2,6-P(2) (fructose 2,6-bisphosphate) is also a glucokinase-binding protein. The role in regulating glucose metabolism insulin secretion pancreatic beta-cells unresolved. We down-regulated endogenous isoforms with siRNA (small interfering RNA) expressed KA (kinase active) KD deficient) variants to distinguish between protein its product, 2,6-P(2), beta-cell function. Human...
The source of new β-cells in adult human pancreas remains incompletely elucidated with recent studies on rodents providing evidence for neogenesis from progenitor cells addition to self-replication. aim this study was investigate the expression pluripotency-associated stem cell markers proliferative cultures derived pancreas. Human pancreatic tissue obtained deceased donors following ethical approval and relative consent. Islet-enriched fraction separated retrieved organ by digestion density...
Islet transplantation has become established as a successful treatment for type 1 diabetes complicated by recurrent severe hypoglycemia. In the UK access been limited to few centrally located units. Our goal was validate quality-assured system safe/effective transport of human islets in and successfully undertake first transplants with transported islets. Pancreases were retrieved from deceased donors north England King's College London using two-layer method (TLM) or University Wisconsin...