A5086234926- Heart Failure Treatment and Management
- Cardiovascular Function and Risk Factors
- Cardiac Fibrosis and Remodeling
- Cancer-related gene regulation
- GDF15 and Related Biomarkers
- Tissue Engineering and Regenerative Medicine
- IL-33, ST2, and ILC Pathways
- Inflammatory Biomarkers in Disease Prognosis
- Atrial Fibrillation Management and Outcomes
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Antiplatelet Therapy and Cardiovascular Diseases
- Congenital heart defects research
- Cardiac Structural Anomalies and Repair
- Clusterin in disease pathology
- Cardiomyopathy and Myosin Studies
- Advanced Proteomics Techniques and Applications
- Salivary Gland Disorders and Functions
- Protease and Inhibitor Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- S100 Proteins and Annexins
- NF-κB Signaling Pathways
- Melanoma and MAPK Pathways
- Studies on Chitinases and Chitosanases
- Signaling Pathways in Disease
University College Dublin
2012-2024
St. Vincent's University Hospital
2019-2022
Heartbeat Trust
2016-2020
Queen's University Belfast
2017
Tampere University
2016
St Michael’s Hospital
2016
King's College London
2016
Hospital General Universitario Gregorio Marañón
2016
Tampere University Hospital
2016
Max Delbrück Center
2010
Aims Differentiation of heart failure with reduced ( HFrEF ) or preserved HFpEF ejection fraction independent echocardiography is challenging in the community. Diagnostic strategies based on monitoring circulating microRNA miRNA levels may prove to be clinical value near future. The aim this study was identify a novel signature that could useful HF diagnostic tool and provide valuable information whether patient has . Methods results MiRNA biomarker discovery carried out three cohorts, no...
Background: Limited knowledge exists of the extent epigenetic alterations, such as DNA methylation, in heart failure (HF). We conducted targeted methylation sequencing to identify alterations coding and noncoding RNA (ncRNA) across different etiological subtypes HF. Methods Results: A bisulfite sequence capture platform was applied extracted from cardiac interventricular septal tissue 30 male HF patients encompassing causes including hypertrophic obstructive cardiomyopathy, ischemic dilated...
Background: The development of heart failure is associated with changes in the size, shape, and structure that has a negative impact on cardiac function. These pathological involve excessive extracellular matrix deposition within myocardial interstitium myocyte hypertrophy. Alterations fibroblast phenotype activity are reprogramming gene transcriptional profiles likely requires epigenetic alterations chromatin structure. aim our work was to investigate potential currently licensed anticancer...
Background Atrial tissue fibrosis is linked to inflammatory cells, yet incompletely understood. A growing body of literature associates peripheral blood levels the antifibrotic hormone BNP (B‐type natriuretic peptide) with atrial fibrillation ( AF ). We investigated relationship between pro‐fibrotic M2 macrophage marker Cluster Differentiation CD )163+, procollagen expression, and gene expression in patients without . Methods Results In a cross‐sectional study design, right was procured from...
Heart failure (HF) screening strategies require biomarkers to predict disease manifestation aid HF surveillance and management programmes. The aim of this study was validate a previous proteomics discovery programme that identified Tetranectin as potential biomarker candidate based on expression level changes in asymptomatic patients at future risk for development. initial consisted 132 patients, comprising (n = 40), no-HF controls 60), cardiac surgery 32). Serum samples were quantified...
Evolutionarily conserved signaling intermediate in Toll pathways (ECSIT) is a protein with roles early development, activation of the transcription factor NF-κB, and production mitochondrial reactive oxygen species (mROS) that facilitates clearance intracellular bacteria like Salmonella. ECSIT also an important assembly for complex I. Unlike murine form Ecsit (mEcsit), we demonstrate here human (hECSIT) highly labile. To explore whether instability hECSIT affects functions previously...
Dilated cardiomyopathy (DCM) is the most common cause of heart failure, with a complex aetiology involving multiple cell types. We aimed to detect cell-specific transcriptomic alterations in DCM through analysis that leveraged recent advancements single-cell analytical tools. Single-cell RNA sequencing (scRNA-seq) data from human cardiac tissue were subjected an updated bioinformatic workflow which unsupervised clustering was paired reference label transfer more comprehensively annotate...
DC are professional APC that instruct T cells during the inflammatory course of EAE. We have previously shown MAPK3 (Erk1) is important for induction T-cell anergy. Our goal was to determine influence on capacity arm responses in autoimmunity. report from Mapk3(-/-) mice a significantly higher membrane expression CD86 and MHC-II and--when loaded with myelin oligodendrocyte glycoprotein--show superior prime naïve towards an phenotype than Mapk3(+/+) DC. Nonetheless as described, were only...
Objective To determine the aetiology of community acquired pneumonia in children presenting to primary care Northern Malawi, and ascertain predictors for identification requiring hospitalisation. Design The BIOmarkers TO diagnose PnEumonia study was a prospective cohort conducted from March June 2016. Setting Primary Malawi. Patients 494 aged 2 –59 months with WHO defined pneumonia. Main outcome(s) measure(s) Number bacterial infection identified sensitivity/specificity markers severity need...
Abstract Aims Dynamic alterations in cardiac DNA methylation have been implicated the development of heart failure (HF) with evidence ischaemic disease (IHD); however, there is limited research into cell specific, sensitive genes that are affected by dysregulated patterns. In this study, we aimed to identify and elucidate their role fibrosis. Methods A multi‐omics integrative analysis was carried out on RNA sequencing HF IHD ( n = 9) versus non‐failing left ventricular tissue, which...
Abstract Aims There is a critical need for better biomarkers so that heart failure can be diagnosed at an earlier stage and with greater accuracy. The purpose of this study was to design robust mass spectrometry (MS)‐based assay the simultaneous measurement panel 35 candidate protein failure, in blood. overall aim evaluate potential clinical utility biomarker prediction cohort 500 patients. Methods results Multiple reaction monitoring (MRM) MS assays were designed Skyline Spectrum Mill...
Abstract Biomarker‐based preventative and monitoring strategies are increasingly used for risk stratification in cardiovascular (CV) disease. The aim of this study was to investigate the utility longitudinal change B‐type natriuretic peptide (BNP) sST2 concentrations predicting incident major adverse CV events (MACE) (heart failure, myocardial infarction, arrhythmia, stroke/transient ischaemic attack death) asymptomatic community‐based patients with factors but without prevalent MACE at...
Inflammation and fibrosis with increased monocyte activation are associated the progression of left ventricular diastolic dysfunction (LVDD) heart failure (HF). Three subclasses exist: classical/inflammatory, intermediate, alternative/anti-inflammatory monocytes. In tissue monocytes differentiate into inflammatory-M1 or regulatory/pro-fibrotic-M2 macrophages. Alternative M2 macrophages have been implicated in pathogenesis atherosclerosis/CAD, MI, HF, etc. Since macrophage polarisation may...
<h3>Introduction</h3> There is ample room for improving the usability of risk prediction tools advancement heart failure (HF) management. New biomarkers and testing technologies, which can diagnose HF, monitor therapy provide information related to subsequent adverse events or mortality affected patients, may drive this change. Blood NTproBNP a well-established biomarker diagnosis monitoring HF. Saliva has recently come under radar as potential new HF – one that more cost-effective, less...