A5063599833- Bacterial Genetics and Biotechnology
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Dermatologic Treatments and Research
- Evolution and Genetic Dynamics
- Corneal Surgery and Treatments
- Heart Failure Treatment and Management
- Genomics and Chromatin Dynamics
- Blood groups and transfusion
- RNA modifications and cancer
- Microbial Metabolic Engineering and Bioproduction
- Antibiotic Resistance in Bacteria
- RNA Research and Splicing
- Hair Growth and Disorders
- Infectious Diseases and Tuberculosis
- Wound Healing and Treatments
- Pressure Ulcer Prevention and Management
- HIV/AIDS drug development and treatment
- Hip disorders and treatments
- Coordination Chemistry and Organometallics
- Cardiac Arrest and Resuscitation
- Venous Thromboembolism Diagnosis and Management
- Sports injuries and prevention
- Geriatric Care and Nursing Homes
- Cardiac Structural Anomalies and Repair
The University of Texas Medical Branch at Galveston
2021-2025
University of Pennsylvania
2025
John Sealy Hospital
2024
ORCID
2021
Nationwide Children's Hospital
2020
Kenyon College
2016-2019
Regardless of the source injury or metabolic dysfunction, fibrosis is a frequent driver liver pathology. Excessive caused by persistent activation hepatic stellate cells (HSCs), which defined myofibroblast (MFA) and epithelial-mesenchymal transition (EMT). Strategies to prevent reverse this HSC phenotype will be critical for successful treatment fibrosis. We have previously shown that full-term, cell-free human amniotic fluid (cfAF) inhibits MFA EMT in fibroblasts vitro. hypothesize cfAF can...
Non-healing venous leg ulcers represent a significant healthcare problem that accounts for about $32 billion of spending in the US alone. Consequently, novel treatment strategies major unmet need. The current study (part one Phase II [NCT04647240]) assesses safety, tolerability and efficacy use cell-free human amniotic fluid treating did not heal following correction reflux. Patients received injections around wound either weekly or biweekly over 12 weeks. Primary outcomes included assessed...
ABSTRACT Escherichia coli K-12 W3110 grows in the presence of membrane-permeant organic acids that can depress cytoplasmic pH and accumulate cytoplasm. We conducted experimental evolution by daily diluting cultures increasing concentrations benzoic acid (up to 20 mM) buffered at external 6.5, a which permeant concentrate By 2,000 generations, clones isolated from evolving populations showed tolerance benzoate but were sensitive chloramphenicol tetracycline. Sixteen grew stationary phase mM...
Alternative splicing is critical for development; however, its role in the specification of three embryonic germ layers poorly understood. By performing RNA-Seq on human stem cells (hESCs) and derived definitive endoderm, cardiac mesoderm, ectoderm cell lineages, we detect distinct alternative programs associated with each lineage. The most prominent program differences are observed between endoderm mesoderm. Integrative multi-omics analyses link lineage-enriched RNA binding protein...
Benzoic acid, a partial uncoupler of the proton motive force (PMF), selects for sensitivity to chloramphenicol and tetracycline during experimental evolution Escherichia coli K-12. Transcriptomes E. isolates evolved with benzoate showed reversal benzoate-dependent regulation, including downregulation multidrug efflux pump genes, gene Gad acid resistance regulon, nitrate reductase genes narHJ, acid-consuming hydrogenase Hyd-3. However, benzoate-evolved strains had increased expression OmpF...
Chronic, non-healing venous ulcers of the lower extremity are often limb-threatening conditions. Their management is characterized by a prolonged and frequently frustrating clinical course that represents an economic burden to both patient healthcare system. During last two decades, thermal ablation underlying incompetent systems has been extensively utilized treat chronic insufficiency. Despite successful correction hypertension, substantial subgroup patients remain affected ulcers, thus...
Objective: Hard-to-heal (chronic) wounds negatively impact patients and are a source of significant strain on the healthcare system economy. These often resistant to standard care (SoC) wound healing approaches due diversity underlying pathologies. Cellular, acellular, matrix-like products, such as amniotic membranes (AM), potential solution these challenges. A growing body evidence suggests that AM may be useful for treatment-resistant wounds; however, limited information is available...
Acute autoimmune hemolytic anemia requires rapid stabilization, typically through the administration of high-dose corticosteroids. However, it is important to consider reactivation latent infection and how immune suppression can interfere with molecular screening tools. In this case report, we present a patient Rheumatoid Arthritis/Systemic Lupus Erythematosus experiencing severe complicated by an unknown tuberculosis infection.
ABSTRACT Benzoate, a partial uncoupler of the proton motive force (PMF), selects for sensitivity to chloramphenicol and tetracycline in Escherichia coli K-12. Genetic mechanisms increased benzoate tolerance decreased drug resistance were analyzed strains isolated from experimental evolution with benzoate. Transcriptomes showed reversal benzoate-dependent regulation, including Gad regulon acid multi-drug (MDR). Benzoate-evolved knocked down nitrate reductase narHJ acid-consuming hydrogenase...
ABSTRACT Alternative splicing is critical for development; however, its role in the specification of three embryonic germ layers poorly understood. By performing RNA-Seq on human stem cells (hESCs) and derived definitive endoderm, cardiac mesoderm, ectoderm cell lineages, we detect distinct alternative programs associated with each lineage. The most prominent program differences are observed between endoderm mesoderm. Integrative multi-omics analyses link lineage-enriched RNA binding protein...
To the Editor: Heart failure is leading cause of hospitalization among elderly, with an estimated yearly cost ~$108 billion dollars globally, which ~$30 spent in United States. 1 Much this burden due to heart failure’s high national readmission rates—the 30-day rate 24% and 1-year 60%. 2 Furthermore, meta-analyses rates show that socioeconomic factors, including lack occupation difficulties receiving care from qualified health professionals, often play a greater role than medical factors...
ABSTRACT Escherichia coli K-12 W3110 grows in the presence of membrane-permeant organic acids that can depress cytoplasmic pH and accumulate cytoplasm. We conducted experimental evolution by daily diluting cultures increasing concentrations benzoic acid (up to 20 mM) buffered at external 6.5, a which permeant concentrate By 2,000 generations, clones isolated from evolving populations showed tolerance benzoate but were sensitive chloramphenicol tetracycline. Sixteen grew stationary phase mM...
Acute autoimmune hemolytic anemia requires rapid stabilization, typically through the administration of high-dose corticosteroids. However, it is important to consider reactivation latent infection and how immune suppression can interfere with molecular screening tools. In this case report, we present a patient Rheumatoid Arthritis/Systemic Lupus Erythematosus experiencing severe complicated by an unknown tuberculosis infection.
In this report, we discuss how autoimmune conditions and associated immunocompromise may impact diagnostic utility of molecular screening tools for mycobacterial infection. Imaging, screening, clinical judgment are required to rule out disease in the immunocompromised, especially an acute setting
In this report, we discuss how autoimmune conditions and associated immunocompromise may impact diagnostic utility of molecular screening tools for mycobacterial infection. Imaging, screening, clinical judgment are required to rule out disease in the immunocompromised, especially an acute setting
Abstract Introduction Heart Failure disease management clinics have been historically successful in reducing complications, but little has done uninsured settings. Methods This is a pilot program for HF patients following recent hospitalization. Uninsured were offered enrollment the clinic during or immediately hospitalization primary diagnosis at our institution 2021. The included twice-weekly visits with interprofessional support. Patients scheduled 16 (2 months of follow-up)...